A comparison of automated urine analyzers cobas 6500, UN 3000-111b and iRICELL 3000 with manual microscopic urinalysis
Objectives: Microscopic examination is essential in urine analysis. This is a simple way to collect informative data but it is also labor-intensive, time-consuming, and requires experienced staff for accurate results and interpretation. Several automated urine analyzers have been introduced for urin...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2021-03-01
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| Series: | Practical Laboratory Medicine |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352551721000032 |
| _version_ | 1818576004998758400 |
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| author | Piraya Tantisaranon Kanyarat Dumkengkhachornwong Peechana Aiadsakun Areerat Hnoonual |
| author_facet | Piraya Tantisaranon Kanyarat Dumkengkhachornwong Peechana Aiadsakun Areerat Hnoonual |
| author_sort | Piraya Tantisaranon |
| collection | DOAJ |
| description | Objectives: Microscopic examination is essential in urine analysis. This is a simple way to collect informative data but it is also labor-intensive, time-consuming, and requires experienced staff for accurate results and interpretation. Several automated urine analyzers have been introduced for urine analysis in medical laboratories. The aim of this study was to assess and compare the performance of the most common three automated urine analyzers, Cobas 6500, UN3000-111b and iRICELL 3000. Methods: A total of 100 routine urine samples were used in the study. Results from the three machines were compared with the routine procedure results including physical, chemical and sediment analysis. Results: There was good correlation of urine physical and chemical analyses between the three analyzers with an overall concordance level of more than 80%. For sediment analysis, the degree of concordance between manual analysis and the three instruments was very good to good for white blood cells, red blood cells and epithelial cells, and moderate for bacteria. There were fair to good agreements between manual microscopy and the three instruments, Cobas 6500, UN3000-111b and iRICELL 3000, for casts (Cohen’s kappa 0.42, 0.38 and 0.62, respectively). Conclusions: The three automated urine analyzers showed similar performances and good correlation with manual microscopy. The results of this study indicate that automated urine analyzers could be used for initial urine testing to reduce high workloads and to save time, but manual microscopic analysis by experienced staff is still necessary to classify urine sediments for confirmation, especially in pathologic specimens. |
| first_indexed | 2024-12-15T00:48:30Z |
| format | Article |
| id | doaj.art-16e11f45882149cba2151c2f5cd21c6f |
| institution | Directory Open Access Journal |
| issn | 2352-5517 |
| language | English |
| last_indexed | 2024-12-15T00:48:30Z |
| publishDate | 2021-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Practical Laboratory Medicine |
| spelling | doaj.art-16e11f45882149cba2151c2f5cd21c6f2022-12-21T22:41:29ZengElsevierPractical Laboratory Medicine2352-55172021-03-0124e00203A comparison of automated urine analyzers cobas 6500, UN 3000-111b and iRICELL 3000 with manual microscopic urinalysisPiraya Tantisaranon0Kanyarat Dumkengkhachornwong1Peechana Aiadsakun2Areerat Hnoonual3Emergency Laboratory, Department of Pathology, Faculty of Medicine, Prince of Songkla University, Songkhla, ThailandEmergency Laboratory, Department of Pathology, Faculty of Medicine, Prince of Songkla University, Songkhla, ThailandClinical Microscopy Laboratory, Department of Pathology, Faculty of Medicine, Prince of Songkla University, Songkhla, ThailandDivision of Human Genetics, Department of Pathology, Faculty of Medicine, Prince of Songkla University, Songkhla, Thailand; Corresponding author. Division of Human Genetics, Department of Pathology, Faculty of Medicine, Prince of Songkla University, Hat Yai, Songkhla, 90110, Thailand.,Objectives: Microscopic examination is essential in urine analysis. This is a simple way to collect informative data but it is also labor-intensive, time-consuming, and requires experienced staff for accurate results and interpretation. Several automated urine analyzers have been introduced for urine analysis in medical laboratories. The aim of this study was to assess and compare the performance of the most common three automated urine analyzers, Cobas 6500, UN3000-111b and iRICELL 3000. Methods: A total of 100 routine urine samples were used in the study. Results from the three machines were compared with the routine procedure results including physical, chemical and sediment analysis. Results: There was good correlation of urine physical and chemical analyses between the three analyzers with an overall concordance level of more than 80%. For sediment analysis, the degree of concordance between manual analysis and the three instruments was very good to good for white blood cells, red blood cells and epithelial cells, and moderate for bacteria. There were fair to good agreements between manual microscopy and the three instruments, Cobas 6500, UN3000-111b and iRICELL 3000, for casts (Cohen’s kappa 0.42, 0.38 and 0.62, respectively). Conclusions: The three automated urine analyzers showed similar performances and good correlation with manual microscopy. The results of this study indicate that automated urine analyzers could be used for initial urine testing to reduce high workloads and to save time, but manual microscopic analysis by experienced staff is still necessary to classify urine sediments for confirmation, especially in pathologic specimens.http://www.sciencedirect.com/science/article/pii/S2352551721000032Urine sedimentAutomated urine analyzerMicroscopicUrinalysis |
| spellingShingle | Piraya Tantisaranon Kanyarat Dumkengkhachornwong Peechana Aiadsakun Areerat Hnoonual A comparison of automated urine analyzers cobas 6500, UN 3000-111b and iRICELL 3000 with manual microscopic urinalysis Practical Laboratory Medicine Urine sediment Automated urine analyzer Microscopic Urinalysis |
| title | A comparison of automated urine analyzers cobas 6500, UN 3000-111b and iRICELL 3000 with manual microscopic urinalysis |
| title_full | A comparison of automated urine analyzers cobas 6500, UN 3000-111b and iRICELL 3000 with manual microscopic urinalysis |
| title_fullStr | A comparison of automated urine analyzers cobas 6500, UN 3000-111b and iRICELL 3000 with manual microscopic urinalysis |
| title_full_unstemmed | A comparison of automated urine analyzers cobas 6500, UN 3000-111b and iRICELL 3000 with manual microscopic urinalysis |
| title_short | A comparison of automated urine analyzers cobas 6500, UN 3000-111b and iRICELL 3000 with manual microscopic urinalysis |
| title_sort | comparison of automated urine analyzers cobas 6500 un 3000 111b and iricell 3000 with manual microscopic urinalysis |
| topic | Urine sediment Automated urine analyzer Microscopic Urinalysis |
| url | http://www.sciencedirect.com/science/article/pii/S2352551721000032 |
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