Novel Bioactive Nanocomposites Containing Calcium Fluoride and Calcium Phosphate with Antibacterial and Low-Shrinkage-Stress Capabilities to Inhibit Dental Caries
Objectives: Composites are commonly used for tooth restorations, but recurrent caries often lead to restoration failures due to polymerization shrinkage-stress-induced marginal leakage. The aims of this research were to: (1) develop novel low-shrinkage-stress (L.S.S.) nanocomposites containing dimet...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-08-01
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| Series: | Bioengineering |
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| Online Access: | https://www.mdpi.com/2306-5354/10/9/991 |
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| author | Abdullah Alhussein Rashed Alsahafi Abdulrahman A. Balhaddad Lamia Mokeem Abraham Schneider Mary-Ann Jabra-Rizk Radi Masri Gary D. Hack Thomas W. Oates Jirun Sun Michael D. Weir Hockin H. K. Xu |
| author_facet | Abdullah Alhussein Rashed Alsahafi Abdulrahman A. Balhaddad Lamia Mokeem Abraham Schneider Mary-Ann Jabra-Rizk Radi Masri Gary D. Hack Thomas W. Oates Jirun Sun Michael D. Weir Hockin H. K. Xu |
| author_sort | Abdullah Alhussein |
| collection | DOAJ |
| description | Objectives: Composites are commonly used for tooth restorations, but recurrent caries often lead to restoration failures due to polymerization shrinkage-stress-induced marginal leakage. The aims of this research were to: (1) develop novel low-shrinkage-stress (L.S.S.) nanocomposites containing dimethylaminododecyl methacrylate (DMADDM) with nanoparticles of calcium fluoride (nCaF<sub>2</sub>) or amorphous calcium phosphate (NACP) for remineralization; (2) investigate antibacterial and cytocompatibility properties. Methods: Nanocomposites were made by mixing triethylene glycol divinylbenzyl ether with urethane dimethacrylate containing 3% DMADDM, 20% nCaF<sub>2</sub>, and 20% NACP. Flexural strength, elastic modulus, antibacterial properties against <i>Streptococcus mutans</i> biofilms, and cytotoxicity against human gingival fibroblasts and dental pulp stem cells were tested. Results: Nanocomposites with DMADDM and nCaF<sub>2</sub> or NACP had flexural strengths matching commercial composite control without bioactivity. The new nanocomposite provided potent antibacterial properties, reducing biofilm CFU by 6 logs, and reducing lactic acid synthesis and metabolic function of biofilms by 90%, compared to controls (<i>p</i> < 0.05). The new nanocomposites produced excellent cell viability matching commercial control (<i>p</i> > 0.05). Conclusions: Bioactive L.S.S. antibacterial nanocomposites with nCaF<sub>2</sub> and NACP had excellent bioactivity without compromising mechanical and cytocompatible properties. The new nanocomposites are promising for a wide range of dental restorations by improving marginal integrity by reducing shrinkage stress, defending tooth structures, and minimizing cariogenic biofilms. |
| first_indexed | 2024-03-10T23:02:05Z |
| format | Article |
| id | doaj.art-16e23ad818d740d3a533b438e285d435 |
| institution | Directory Open Access Journal |
| issn | 2306-5354 |
| language | English |
| last_indexed | 2024-03-10T23:02:05Z |
| publishDate | 2023-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Bioengineering |
| spelling | doaj.art-16e23ad818d740d3a533b438e285d4352023-11-19T09:36:10ZengMDPI AGBioengineering2306-53542023-08-0110999110.3390/bioengineering10090991Novel Bioactive Nanocomposites Containing Calcium Fluoride and Calcium Phosphate with Antibacterial and Low-Shrinkage-Stress Capabilities to Inhibit Dental CariesAbdullah Alhussein0Rashed Alsahafi1Abdulrahman A. Balhaddad2Lamia Mokeem3Abraham Schneider4Mary-Ann Jabra-Rizk5Radi Masri6Gary D. Hack7Thomas W. Oates8Jirun Sun9Michael D. Weir10Hockin H. K. Xu11PhD Program in Dental Biomedical Sciences, University of Maryland School of Dentistry, Baltimore, MD 21201, USADepartment of Restorative Dental Sciences, Umm Al-Qura University, College of Dentistry, Makkah 24211, Saudi ArabiaDepartment of Restorative Dental Sciences, College of Dentistry, Imam Abdulrahman Bin Faisal University, Dammam 31441, Saudi ArabiaPhD Program in Dental Biomedical Sciences, University of Maryland School of Dentistry, Baltimore, MD 21201, USADepartment of Oncology and Diagnostic Sciences, University of Maryland School of Dentistry, Baltimore, MD 21201, USADepartment of Oncology and Diagnostic Sciences, University of Maryland School of Dentistry, Baltimore, MD 21201, USADepartment of Advanced Oral Sciences and Therapeutics, University of Maryland School of Dentistry, Baltimore, MD 21201, USADepartment of Advanced Oral Sciences and Therapeutics, University of Maryland School of Dentistry, Baltimore, MD 21201, USADepartment of Advanced Oral Sciences and Therapeutics, University of Maryland School of Dentistry, Baltimore, MD 21201, USAThe Forsyth Institute, Harvard School of Dental Medicine Affiliate, Cambridge, MA 02142, USADepartment of Advanced Oral Sciences and Therapeutics, University of Maryland School of Dentistry, Baltimore, MD 21201, USADepartment of Advanced Oral Sciences and Therapeutics, University of Maryland School of Dentistry, Baltimore, MD 21201, USAObjectives: Composites are commonly used for tooth restorations, but recurrent caries often lead to restoration failures due to polymerization shrinkage-stress-induced marginal leakage. The aims of this research were to: (1) develop novel low-shrinkage-stress (L.S.S.) nanocomposites containing dimethylaminododecyl methacrylate (DMADDM) with nanoparticles of calcium fluoride (nCaF<sub>2</sub>) or amorphous calcium phosphate (NACP) for remineralization; (2) investigate antibacterial and cytocompatibility properties. Methods: Nanocomposites were made by mixing triethylene glycol divinylbenzyl ether with urethane dimethacrylate containing 3% DMADDM, 20% nCaF<sub>2</sub>, and 20% NACP. Flexural strength, elastic modulus, antibacterial properties against <i>Streptococcus mutans</i> biofilms, and cytotoxicity against human gingival fibroblasts and dental pulp stem cells were tested. Results: Nanocomposites with DMADDM and nCaF<sub>2</sub> or NACP had flexural strengths matching commercial composite control without bioactivity. The new nanocomposite provided potent antibacterial properties, reducing biofilm CFU by 6 logs, and reducing lactic acid synthesis and metabolic function of biofilms by 90%, compared to controls (<i>p</i> < 0.05). The new nanocomposites produced excellent cell viability matching commercial control (<i>p</i> > 0.05). Conclusions: Bioactive L.S.S. antibacterial nanocomposites with nCaF<sub>2</sub> and NACP had excellent bioactivity without compromising mechanical and cytocompatible properties. The new nanocomposites are promising for a wide range of dental restorations by improving marginal integrity by reducing shrinkage stress, defending tooth structures, and minimizing cariogenic biofilms.https://www.mdpi.com/2306-5354/10/9/991low polymerization stressnanocompositeremineralizationbio-interactiveantibiofilmoral biofilms |
| spellingShingle | Abdullah Alhussein Rashed Alsahafi Abdulrahman A. Balhaddad Lamia Mokeem Abraham Schneider Mary-Ann Jabra-Rizk Radi Masri Gary D. Hack Thomas W. Oates Jirun Sun Michael D. Weir Hockin H. K. Xu Novel Bioactive Nanocomposites Containing Calcium Fluoride and Calcium Phosphate with Antibacterial and Low-Shrinkage-Stress Capabilities to Inhibit Dental Caries Bioengineering low polymerization stress nanocomposite remineralization bio-interactive antibiofilm oral biofilms |
| title | Novel Bioactive Nanocomposites Containing Calcium Fluoride and Calcium Phosphate with Antibacterial and Low-Shrinkage-Stress Capabilities to Inhibit Dental Caries |
| title_full | Novel Bioactive Nanocomposites Containing Calcium Fluoride and Calcium Phosphate with Antibacterial and Low-Shrinkage-Stress Capabilities to Inhibit Dental Caries |
| title_fullStr | Novel Bioactive Nanocomposites Containing Calcium Fluoride and Calcium Phosphate with Antibacterial and Low-Shrinkage-Stress Capabilities to Inhibit Dental Caries |
| title_full_unstemmed | Novel Bioactive Nanocomposites Containing Calcium Fluoride and Calcium Phosphate with Antibacterial and Low-Shrinkage-Stress Capabilities to Inhibit Dental Caries |
| title_short | Novel Bioactive Nanocomposites Containing Calcium Fluoride and Calcium Phosphate with Antibacterial and Low-Shrinkage-Stress Capabilities to Inhibit Dental Caries |
| title_sort | novel bioactive nanocomposites containing calcium fluoride and calcium phosphate with antibacterial and low shrinkage stress capabilities to inhibit dental caries |
| topic | low polymerization stress nanocomposite remineralization bio-interactive antibiofilm oral biofilms |
| url | https://www.mdpi.com/2306-5354/10/9/991 |
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