An optimized high-performance liquid chromatography (HPLC) method for benzoylmesaconine determination in <it>Radix Aconiti Lateralis Preparata </it>(<it>Fuzi</it>, aconite roots) and its products
<p>Abstract</p> <p>Background</p> <p>Benzoylmesaconine (BMA) is the main <it>Aconitum </it>alkaloid in <it>Radix Aconiti Lateralis Preparata </it>(<it>Fuzi</it>, aconite roots) with potent pharmacological activities, such as analgesia...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2008-05-01
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| Series: | Chinese Medicine |
| Online Access: | http://www.cmjournal.org/content/3/1/6 |
| _version_ | 1818691156856274944 |
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| author | Xu Hongxi Liu Zhongqiu Wong Yuen Zhou Hua Xie Ying Jiang Zhihong Liu Liang |
| author_facet | Xu Hongxi Liu Zhongqiu Wong Yuen Zhou Hua Xie Ying Jiang Zhihong Liu Liang |
| author_sort | Xu Hongxi |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Benzoylmesaconine (BMA) is the main <it>Aconitum </it>alkaloid in <it>Radix Aconiti Lateralis Preparata </it>(<it>Fuzi</it>, aconite roots) with potent pharmacological activities, such as analgesia and anti-inflammation. The present study developed a simple and reliable method using BMA as a marker compound for the quality control of processed aconite roots and their products.</p> <p>Methods</p> <p>After extraction, a high-performance liquid chromatography (HPLC) determination of BMA was conducted on a RP-C<sub>18 </sub>column by gradient elution with acetonitrile and aqueous phase, containing 0.1% phosphoric acid adjusted with triethylamine to pH 3.0.</p> <p>Results</p> <p>A distinct peak profile was obtained and separation of BMA was achieved. Method validation showed that the relative standard deviations (RSDs) of the precision of BMA in all intra-day and inter-day assays were less than 1.36%, and that the average recovery rate was 96.95%. Quantitative analysis of BMA showed that the content of BMA varied significantly in processed aconite roots and their products.</p> <p>Conclusion</p> <p>This HPLC method using BMA as a marker compound is applicable to the quality control of processed aconite roots and their products.</p> |
| first_indexed | 2024-12-17T12:37:25Z |
| format | Article |
| id | doaj.art-16e5eb4c3d7d4ba08b2b6dbe8443f236 |
| institution | Directory Open Access Journal |
| issn | 1749-8546 |
| language | English |
| last_indexed | 2024-12-17T12:37:25Z |
| publishDate | 2008-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Chinese Medicine |
| spelling | doaj.art-16e5eb4c3d7d4ba08b2b6dbe8443f2362022-12-21T21:48:13ZengBMCChinese Medicine1749-85462008-05-0131610.1186/1749-8546-3-6An optimized high-performance liquid chromatography (HPLC) method for benzoylmesaconine determination in <it>Radix Aconiti Lateralis Preparata </it>(<it>Fuzi</it>, aconite roots) and its productsXu HongxiLiu ZhongqiuWong YuenZhou HuaXie YingJiang ZhihongLiu Liang<p>Abstract</p> <p>Background</p> <p>Benzoylmesaconine (BMA) is the main <it>Aconitum </it>alkaloid in <it>Radix Aconiti Lateralis Preparata </it>(<it>Fuzi</it>, aconite roots) with potent pharmacological activities, such as analgesia and anti-inflammation. The present study developed a simple and reliable method using BMA as a marker compound for the quality control of processed aconite roots and their products.</p> <p>Methods</p> <p>After extraction, a high-performance liquid chromatography (HPLC) determination of BMA was conducted on a RP-C<sub>18 </sub>column by gradient elution with acetonitrile and aqueous phase, containing 0.1% phosphoric acid adjusted with triethylamine to pH 3.0.</p> <p>Results</p> <p>A distinct peak profile was obtained and separation of BMA was achieved. Method validation showed that the relative standard deviations (RSDs) of the precision of BMA in all intra-day and inter-day assays were less than 1.36%, and that the average recovery rate was 96.95%. Quantitative analysis of BMA showed that the content of BMA varied significantly in processed aconite roots and their products.</p> <p>Conclusion</p> <p>This HPLC method using BMA as a marker compound is applicable to the quality control of processed aconite roots and their products.</p>http://www.cmjournal.org/content/3/1/6 |
| spellingShingle | Xu Hongxi Liu Zhongqiu Wong Yuen Zhou Hua Xie Ying Jiang Zhihong Liu Liang An optimized high-performance liquid chromatography (HPLC) method for benzoylmesaconine determination in <it>Radix Aconiti Lateralis Preparata </it>(<it>Fuzi</it>, aconite roots) and its products Chinese Medicine |
| title | An optimized high-performance liquid chromatography (HPLC) method for benzoylmesaconine determination in <it>Radix Aconiti Lateralis Preparata </it>(<it>Fuzi</it>, aconite roots) and its products |
| title_full | An optimized high-performance liquid chromatography (HPLC) method for benzoylmesaconine determination in <it>Radix Aconiti Lateralis Preparata </it>(<it>Fuzi</it>, aconite roots) and its products |
| title_fullStr | An optimized high-performance liquid chromatography (HPLC) method for benzoylmesaconine determination in <it>Radix Aconiti Lateralis Preparata </it>(<it>Fuzi</it>, aconite roots) and its products |
| title_full_unstemmed | An optimized high-performance liquid chromatography (HPLC) method for benzoylmesaconine determination in <it>Radix Aconiti Lateralis Preparata </it>(<it>Fuzi</it>, aconite roots) and its products |
| title_short | An optimized high-performance liquid chromatography (HPLC) method for benzoylmesaconine determination in <it>Radix Aconiti Lateralis Preparata </it>(<it>Fuzi</it>, aconite roots) and its products |
| title_sort | optimized high performance liquid chromatography hplc method for benzoylmesaconine determination in it radix aconiti lateralis preparata it it fuzi it aconite roots and its products |
| url | http://www.cmjournal.org/content/3/1/6 |
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