Prunus spinosa L. leaf extracts: polyphenol profile and bioactivities
Prunus spinosa leaf extracts in solvents of different polarity (water, ethanol and acetone), their phenol, flavonoid and anthocyanin contents and biological properties were the object of this study. The richest in phenols as well as in flavonoids was acetone extract with 181.19 mg GAE and 80.10 mg Q...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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AcademicPres
2021-03-01
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| Series: | Notulae Botanicae Horti Agrobotanici Cluj-Napoca |
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| Online Access: | https://www.notulaebotanicae.ro/index.php/nbha/article/view/12137 |
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| author | Ivona VELIČKOVIĆ Željko ŽIŽAK Nemanja RAJČEVIĆ Marija IVANOV Marina SOKOVIĆ Petar D. MARIN Slavica GRUJIĆ |
| author_facet | Ivona VELIČKOVIĆ Željko ŽIŽAK Nemanja RAJČEVIĆ Marija IVANOV Marina SOKOVIĆ Petar D. MARIN Slavica GRUJIĆ |
| author_sort | Ivona VELIČKOVIĆ |
| collection | DOAJ |
| description | Prunus spinosa leaf extracts in solvents of different polarity (water, ethanol and acetone), their phenol, flavonoid and anthocyanin contents and biological properties were the object of this study. The richest in phenols as well as in flavonoids was acetone extract with 181.19 mg GAE and 80.10 mg QE per gram of dry extract, respectively. Moreover, the quantity of anthocyanins obtained by HPLC analysis was also the highest in acetone sample. Examined samples possessed antioxidant properties evaluated through four in vitro assays (DPPH, ABTS, FRAP and TRC). The acetone extract was proved to be the best antioxidant among tested samples, which could be ascribed to polyphenols, especially anthocyanins. The aqueous and the ethanol extract exhibited antibacterial effects, being particularly active against B. cereus and E. cloacae. T. viride, P. funiculosum, P. ochrochloron, P. verrucosum var. cyclopium were the most susceptible among fungal microorganisms examined. Both, the aqueous and the ethanol extract expressed inhibitory activity towards enzymes linked to diabetes mellitus type II. Additionally, the ethanol extract showed significantly higher potential in inhibiting α-glucosidase than the drug used as the positive control. Furthermore, the aqueous sample revealed antitumor effects on following malignant cell lines: HeLa, K562 and MDA-MB-453. The results presented herein suggest that P. spinosa leaves should be considered as a natural source of bioactive compounds with potential application in phytopharmacy and food industry. |
| first_indexed | 2024-12-19T13:28:12Z |
| format | Article |
| id | doaj.art-16e613ee2a2247d8b69f8465ec587568 |
| institution | Directory Open Access Journal |
| issn | 0255-965X 1842-4309 |
| language | English |
| last_indexed | 2024-12-19T13:28:12Z |
| publishDate | 2021-03-01 |
| publisher | AcademicPres |
| record_format | Article |
| series | Notulae Botanicae Horti Agrobotanici Cluj-Napoca |
| spelling | doaj.art-16e613ee2a2247d8b69f8465ec5875682022-12-21T20:19:29ZengAcademicPresNotulae Botanicae Horti Agrobotanici Cluj-Napoca0255-965X1842-43092021-03-0149110.15835/nbha49112137Prunus spinosa L. leaf extracts: polyphenol profile and bioactivitiesIvona VELIČKOVIĆ0Željko ŽIŽAK1Nemanja RAJČEVIĆ2Marija IVANOV3Marina SOKOVIĆ4Petar D. MARIN5Slavica GRUJIĆ6University of Belgrade, Faculty of Biology, Institute of Botany and Botanical Garden “Jevremovac”, Studentski trg 3, 11000 BelgradeInstitute of Oncology and Radiology of Serbia, Pasterova 14, 11000 BelgradeUniversity of Belgrade, Faculty of Biology, Institute of Botany and Botanical Garden “Jevremovac”, Studentski trg 3, 11000 BelgradeUniversity of Belgrade, Institute for Biological Research “Siniša Stanković”–National Institute of Republic of Serbia, Bulevar Despota Stefana 142, 11000 BelgradeUniversity of Belgrade, Institute for Biological Research “Siniša Stanković”–National Institute of Republic of Serbia, Bulevar Despota Stefana 142, 11000 BelgradeUniversity of Belgrade, Faculty of Biology, Institute of Botany and Botanical Garden “Jevremovac”, Studentski trg 3, 11000 BelgradeUniversity of Belgrade, Faculty of Biology, Institute of Botany and Botanical Garden “Jevremovac”, Studentski trg 3, 11000 BelgradePrunus spinosa leaf extracts in solvents of different polarity (water, ethanol and acetone), their phenol, flavonoid and anthocyanin contents and biological properties were the object of this study. The richest in phenols as well as in flavonoids was acetone extract with 181.19 mg GAE and 80.10 mg QE per gram of dry extract, respectively. Moreover, the quantity of anthocyanins obtained by HPLC analysis was also the highest in acetone sample. Examined samples possessed antioxidant properties evaluated through four in vitro assays (DPPH, ABTS, FRAP and TRC). The acetone extract was proved to be the best antioxidant among tested samples, which could be ascribed to polyphenols, especially anthocyanins. The aqueous and the ethanol extract exhibited antibacterial effects, being particularly active against B. cereus and E. cloacae. T. viride, P. funiculosum, P. ochrochloron, P. verrucosum var. cyclopium were the most susceptible among fungal microorganisms examined. Both, the aqueous and the ethanol extract expressed inhibitory activity towards enzymes linked to diabetes mellitus type II. Additionally, the ethanol extract showed significantly higher potential in inhibiting α-glucosidase than the drug used as the positive control. Furthermore, the aqueous sample revealed antitumor effects on following malignant cell lines: HeLa, K562 and MDA-MB-453. The results presented herein suggest that P. spinosa leaves should be considered as a natural source of bioactive compounds with potential application in phytopharmacy and food industry.https://www.notulaebotanicae.ro/index.php/nbha/article/view/12137anthocyanins; antioxidant activity; antitumor activity; enzyme-inhibitory activity; leaf extracts; Prunus spinosa |
| spellingShingle | Ivona VELIČKOVIĆ Željko ŽIŽAK Nemanja RAJČEVIĆ Marija IVANOV Marina SOKOVIĆ Petar D. MARIN Slavica GRUJIĆ Prunus spinosa L. leaf extracts: polyphenol profile and bioactivities Notulae Botanicae Horti Agrobotanici Cluj-Napoca anthocyanins; antioxidant activity; antitumor activity; enzyme-inhibitory activity; leaf extracts; Prunus spinosa |
| title | Prunus spinosa L. leaf extracts: polyphenol profile and bioactivities |
| title_full | Prunus spinosa L. leaf extracts: polyphenol profile and bioactivities |
| title_fullStr | Prunus spinosa L. leaf extracts: polyphenol profile and bioactivities |
| title_full_unstemmed | Prunus spinosa L. leaf extracts: polyphenol profile and bioactivities |
| title_short | Prunus spinosa L. leaf extracts: polyphenol profile and bioactivities |
| title_sort | prunus spinosa l leaf extracts polyphenol profile and bioactivities |
| topic | anthocyanins; antioxidant activity; antitumor activity; enzyme-inhibitory activity; leaf extracts; Prunus spinosa |
| url | https://www.notulaebotanicae.ro/index.php/nbha/article/view/12137 |
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