Comparison of the methylglyoxal scavenging effects of kaempferol and glutathione and the consequences for the toxicity of methylglyoxal in SH-SY5Y cells
This study aimed to characterize the methylglyoxal (MGO) scavenging capacity of glutathione (GSH) and kaempferol in more detail with special emphasis on the possible reversible nature of the adduct formation and their competition for MGO, and the safety consequences of their MGO-scavenging effects....
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-12-01
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| Series: | Food Chemistry: X |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2590157523003632 |
| _version_ | 1797383702828810240 |
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| author | Liang Zheng Wouter Bakker Ignacio Miro Estruch Frances Widjaja Ivonne M.C.M. Rietjens |
| author_facet | Liang Zheng Wouter Bakker Ignacio Miro Estruch Frances Widjaja Ivonne M.C.M. Rietjens |
| author_sort | Liang Zheng |
| collection | DOAJ |
| description | This study aimed to characterize the methylglyoxal (MGO) scavenging capacity of glutathione (GSH) and kaempferol in more detail with special emphasis on the possible reversible nature of the adduct formation and their competition for MGO, and the safety consequences of their MGO-scavenging effects. GSH showed immediate and concentration-dependent MGO-scavenging effects, while the scavenging effects by kaempferol appeared concentration- but also time-dependent, with stable adducts formed over time. The GSH adduct gradually disappeared in a competition reaction with kaempferol, and kaempferol became the preferred scavenger over time. Furthermore, the scavenging of MGO by kaempferol provided better protection than GSH against extracellular MGO in SH-SY5Y cells. It is concluded that flavonoids like kaempferol provide better scavengers for food-borne MGO than thiol-based scavengers such as GSH, while, given the endogenous concentrations of both scavengers and the detoxification of the GSH-MGO adduct by the glyoxalase system, GSH will be dominant for intracellular MGO protection. |
| first_indexed | 2024-03-08T21:24:52Z |
| format | Article |
| id | doaj.art-16e662c6762848009b649300acdce628 |
| institution | Directory Open Access Journal |
| issn | 2590-1575 |
| language | English |
| last_indexed | 2024-03-08T21:24:52Z |
| publishDate | 2023-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Food Chemistry: X |
| spelling | doaj.art-16e662c6762848009b649300acdce6282023-12-21T07:36:35ZengElsevierFood Chemistry: X2590-15752023-12-0120100920Comparison of the methylglyoxal scavenging effects of kaempferol and glutathione and the consequences for the toxicity of methylglyoxal in SH-SY5Y cellsLiang Zheng0Wouter Bakker1Ignacio Miro Estruch2Frances Widjaja3Ivonne M.C.M. Rietjens4Corresponding author.; Division of Toxicology, Wageningen University and Research, Stippeneng 4, 6708 WE Wageningen, The NetherlandsDivision of Toxicology, Wageningen University and Research, Stippeneng 4, 6708 WE Wageningen, The NetherlandsDivision of Toxicology, Wageningen University and Research, Stippeneng 4, 6708 WE Wageningen, The NetherlandsDivision of Toxicology, Wageningen University and Research, Stippeneng 4, 6708 WE Wageningen, The NetherlandsDivision of Toxicology, Wageningen University and Research, Stippeneng 4, 6708 WE Wageningen, The NetherlandsThis study aimed to characterize the methylglyoxal (MGO) scavenging capacity of glutathione (GSH) and kaempferol in more detail with special emphasis on the possible reversible nature of the adduct formation and their competition for MGO, and the safety consequences of their MGO-scavenging effects. GSH showed immediate and concentration-dependent MGO-scavenging effects, while the scavenging effects by kaempferol appeared concentration- but also time-dependent, with stable adducts formed over time. The GSH adduct gradually disappeared in a competition reaction with kaempferol, and kaempferol became the preferred scavenger over time. Furthermore, the scavenging of MGO by kaempferol provided better protection than GSH against extracellular MGO in SH-SY5Y cells. It is concluded that flavonoids like kaempferol provide better scavengers for food-borne MGO than thiol-based scavengers such as GSH, while, given the endogenous concentrations of both scavengers and the detoxification of the GSH-MGO adduct by the glyoxalase system, GSH will be dominant for intracellular MGO protection.http://www.sciencedirect.com/science/article/pii/S2590157523003632MethylglyoxalKaempferolGlutathioneAdduct formationCytotoxicity |
| spellingShingle | Liang Zheng Wouter Bakker Ignacio Miro Estruch Frances Widjaja Ivonne M.C.M. Rietjens Comparison of the methylglyoxal scavenging effects of kaempferol and glutathione and the consequences for the toxicity of methylglyoxal in SH-SY5Y cells Food Chemistry: X Methylglyoxal Kaempferol Glutathione Adduct formation Cytotoxicity |
| title | Comparison of the methylglyoxal scavenging effects of kaempferol and glutathione and the consequences for the toxicity of methylglyoxal in SH-SY5Y cells |
| title_full | Comparison of the methylglyoxal scavenging effects of kaempferol and glutathione and the consequences for the toxicity of methylglyoxal in SH-SY5Y cells |
| title_fullStr | Comparison of the methylglyoxal scavenging effects of kaempferol and glutathione and the consequences for the toxicity of methylglyoxal in SH-SY5Y cells |
| title_full_unstemmed | Comparison of the methylglyoxal scavenging effects of kaempferol and glutathione and the consequences for the toxicity of methylglyoxal in SH-SY5Y cells |
| title_short | Comparison of the methylglyoxal scavenging effects of kaempferol and glutathione and the consequences for the toxicity of methylglyoxal in SH-SY5Y cells |
| title_sort | comparison of the methylglyoxal scavenging effects of kaempferol and glutathione and the consequences for the toxicity of methylglyoxal in sh sy5y cells |
| topic | Methylglyoxal Kaempferol Glutathione Adduct formation Cytotoxicity |
| url | http://www.sciencedirect.com/science/article/pii/S2590157523003632 |
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