Bovine Derived in vitro Cultures Generate Heterogeneous Populations of Antigen Presenting Cells
Antigen presenting cells (APC) of the mononuclear phagocytic system include dendritic cells (DCs) and macrophages (Macs) which are essential mediators of innate and adaptive immune responses. Many of the biological functions attributed to these cell subsets have been elucidated using models that uti...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2019-03-01
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| Series: | Frontiers in Immunology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/article/10.3389/fimmu.2019.00612/full |
| _version_ | 1818335010949693440 |
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| author | Efrain Guzman Myriam Pujol Paolo Ribeca Maria Montoya Maria Montoya |
| author_facet | Efrain Guzman Myriam Pujol Paolo Ribeca Maria Montoya Maria Montoya |
| author_sort | Efrain Guzman |
| collection | DOAJ |
| description | Antigen presenting cells (APC) of the mononuclear phagocytic system include dendritic cells (DCs) and macrophages (Macs) which are essential mediators of innate and adaptive immune responses. Many of the biological functions attributed to these cell subsets have been elucidated using models that utilize in vitro-matured cells derived from common progenitors. However, it has recently been shown that monocyte culture systems generate heterogeneous populations of cells, DCs, and Macs. In light of these findings, we analyzed the most commonly used bovine in vitro-derived APC models and compared them to bona fide DCs. Here, we show that bovine monocyte-derived DCs and Macs can be differentiated on the basis of CD11c and MHC class II (MHCII) expression and that in vitro conditions generate a heterologous group of both DCs and Macs with defined and specific biological activities. In addition, skin-migrating macrophages present in the bovine afferent lymph were identified and phenotyped for the first time. RNA sequencing analyses showed that these monophagocytic cells have distinct transcriptomic profiles similar to those described in other species. These results have important implications for the interpretation of data obtained using in vitro systems. |
| first_indexed | 2024-12-13T14:16:38Z |
| format | Article |
| id | doaj.art-16e79cb6d74e4cf1885204b7171ad266 |
| institution | Directory Open Access Journal |
| issn | 1664-3224 |
| language | English |
| last_indexed | 2024-12-13T14:16:38Z |
| publishDate | 2019-03-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Immunology |
| spelling | doaj.art-16e79cb6d74e4cf1885204b7171ad2662022-12-21T23:42:17ZengFrontiers Media S.A.Frontiers in Immunology1664-32242019-03-011010.3389/fimmu.2019.00612419846Bovine Derived in vitro Cultures Generate Heterogeneous Populations of Antigen Presenting CellsEfrain Guzman0Myriam Pujol1Paolo Ribeca2Maria Montoya3Maria Montoya4The Pirbright Institute, Woking, United KingdomDoctoral Program in Agronomy Forestry and Veterinary Sciences, Universidad de Chile, Santiago, ChileThe Pirbright Institute, Woking, United KingdomThe Pirbright Institute, Woking, United KingdomCentro de Investigaciones Biológicas (CIB - CSIC), Madrid, SpainAntigen presenting cells (APC) of the mononuclear phagocytic system include dendritic cells (DCs) and macrophages (Macs) which are essential mediators of innate and adaptive immune responses. Many of the biological functions attributed to these cell subsets have been elucidated using models that utilize in vitro-matured cells derived from common progenitors. However, it has recently been shown that monocyte culture systems generate heterogeneous populations of cells, DCs, and Macs. In light of these findings, we analyzed the most commonly used bovine in vitro-derived APC models and compared them to bona fide DCs. Here, we show that bovine monocyte-derived DCs and Macs can be differentiated on the basis of CD11c and MHC class II (MHCII) expression and that in vitro conditions generate a heterologous group of both DCs and Macs with defined and specific biological activities. In addition, skin-migrating macrophages present in the bovine afferent lymph were identified and phenotyped for the first time. RNA sequencing analyses showed that these monophagocytic cells have distinct transcriptomic profiles similar to those described in other species. These results have important implications for the interpretation of data obtained using in vitro systems.https://www.frontiersin.org/article/10.3389/fimmu.2019.00612/fullbovine dendritic cellsbovine monocytesbovine macrophagesbovine antigen presenting cellsin vitro-derived antigen presenting cells |
| spellingShingle | Efrain Guzman Myriam Pujol Paolo Ribeca Maria Montoya Maria Montoya Bovine Derived in vitro Cultures Generate Heterogeneous Populations of Antigen Presenting Cells Frontiers in Immunology bovine dendritic cells bovine monocytes bovine macrophages bovine antigen presenting cells in vitro-derived antigen presenting cells |
| title | Bovine Derived in vitro Cultures Generate Heterogeneous Populations of Antigen Presenting Cells |
| title_full | Bovine Derived in vitro Cultures Generate Heterogeneous Populations of Antigen Presenting Cells |
| title_fullStr | Bovine Derived in vitro Cultures Generate Heterogeneous Populations of Antigen Presenting Cells |
| title_full_unstemmed | Bovine Derived in vitro Cultures Generate Heterogeneous Populations of Antigen Presenting Cells |
| title_short | Bovine Derived in vitro Cultures Generate Heterogeneous Populations of Antigen Presenting Cells |
| title_sort | bovine derived in vitro cultures generate heterogeneous populations of antigen presenting cells |
| topic | bovine dendritic cells bovine monocytes bovine macrophages bovine antigen presenting cells in vitro-derived antigen presenting cells |
| url | https://www.frontiersin.org/article/10.3389/fimmu.2019.00612/full |
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