<i>Staphylococcus aureus</i> Isolated from the Oral Cavity: Phage Susceptibility in Relation to Antibiotic Resistance
Nowadays, research on bacteriophage therapy and its potential use in combination with antibiotics has been gaining momentum. One hundred and ten oral <i>Staphylococcus aureus</i> isolates were phage-typed and their antibiotic resistance was determined by standard and molecular methods. T...
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| Language: | English |
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MDPI AG
2021-10-01
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| Series: | Antibiotics |
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| Online Access: | https://www.mdpi.com/2079-6382/10/11/1329 |
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| author | Katarzyna Garbacz Ewa Kwapisz Lidia Piechowicz Maria Wierzbowska |
| author_facet | Katarzyna Garbacz Ewa Kwapisz Lidia Piechowicz Maria Wierzbowska |
| author_sort | Katarzyna Garbacz |
| collection | DOAJ |
| description | Nowadays, research on bacteriophage therapy and its potential use in combination with antibiotics has been gaining momentum. One hundred and ten oral <i>Staphylococcus aureus</i> isolates were phage-typed and their antibiotic resistance was determined by standard and molecular methods. The prevalence of MSSA and MRSA strains was 89.1% and 10.9%, respectively. Nearly all (91.8%) analyzed isolates, whether MSSA or MRSA, were susceptible to the phages used from the international set. The highest lytic activity showed phages 79 and 52 A from lytic group I. The predominant phage groups were mixed, the I+III group and a mixed group containing phages from at least three various lytic groups. <i>S. aureus</i> strains sensitive to phage group I were usually resistant to penicillin and susceptible to ciprofloxacin, whereas the strains typeable with group V or group V with the 95 phage were susceptible to most antibiotics. Epidemic CA-MRSA strains (SCC<i>mec</i>IV) of phage type 80/81 carried Panton–Valentine leucocidin genes. Considering the high sensitivity of oral <i>S. aureus</i> to the analyzed phages and the promising results of phage therapies reported by other authors, phage cocktails or phage-antibiotic combinations may potentially find applications in both the prevention and eradication of staphylococcal infections. |
| first_indexed | 2024-03-10T05:45:49Z |
| format | Article |
| id | doaj.art-16ea4c397ca24dee9cf78e5d46a5f7c4 |
| institution | Directory Open Access Journal |
| issn | 2079-6382 |
| language | English |
| last_indexed | 2024-03-10T05:45:49Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Antibiotics |
| spelling | doaj.art-16ea4c397ca24dee9cf78e5d46a5f7c42023-11-22T22:10:01ZengMDPI AGAntibiotics2079-63822021-10-011011132910.3390/antibiotics10111329<i>Staphylococcus aureus</i> Isolated from the Oral Cavity: Phage Susceptibility in Relation to Antibiotic ResistanceKatarzyna Garbacz0Ewa Kwapisz1Lidia Piechowicz2Maria Wierzbowska3Department of Oral Microbiology, Medical Faculty, Medical University of Gdansk, 80-204 Gdansk, PolandDepartment of Oral Microbiology, Medical Faculty, Medical University of Gdansk, 80-204 Gdansk, PolandDepartment of Medical Microbiology, Medical Faculty, Medical University of Gdansk, 80-204 Gdansk, PolandDepartment of Oral Microbiology, Medical Faculty, Medical University of Gdansk, 80-204 Gdansk, PolandNowadays, research on bacteriophage therapy and its potential use in combination with antibiotics has been gaining momentum. One hundred and ten oral <i>Staphylococcus aureus</i> isolates were phage-typed and their antibiotic resistance was determined by standard and molecular methods. The prevalence of MSSA and MRSA strains was 89.1% and 10.9%, respectively. Nearly all (91.8%) analyzed isolates, whether MSSA or MRSA, were susceptible to the phages used from the international set. The highest lytic activity showed phages 79 and 52 A from lytic group I. The predominant phage groups were mixed, the I+III group and a mixed group containing phages from at least three various lytic groups. <i>S. aureus</i> strains sensitive to phage group I were usually resistant to penicillin and susceptible to ciprofloxacin, whereas the strains typeable with group V or group V with the 95 phage were susceptible to most antibiotics. Epidemic CA-MRSA strains (SCC<i>mec</i>IV) of phage type 80/81 carried Panton–Valentine leucocidin genes. Considering the high sensitivity of oral <i>S. aureus</i> to the analyzed phages and the promising results of phage therapies reported by other authors, phage cocktails or phage-antibiotic combinations may potentially find applications in both the prevention and eradication of staphylococcal infections.https://www.mdpi.com/2079-6382/10/11/1329bacteriophagephage therapy<i>Staphylococcus aureus</i>oral cavityantibiotic resistanceMRSA |
| spellingShingle | Katarzyna Garbacz Ewa Kwapisz Lidia Piechowicz Maria Wierzbowska <i>Staphylococcus aureus</i> Isolated from the Oral Cavity: Phage Susceptibility in Relation to Antibiotic Resistance Antibiotics bacteriophage phage therapy <i>Staphylococcus aureus</i> oral cavity antibiotic resistance MRSA |
| title | <i>Staphylococcus aureus</i> Isolated from the Oral Cavity: Phage Susceptibility in Relation to Antibiotic Resistance |
| title_full | <i>Staphylococcus aureus</i> Isolated from the Oral Cavity: Phage Susceptibility in Relation to Antibiotic Resistance |
| title_fullStr | <i>Staphylococcus aureus</i> Isolated from the Oral Cavity: Phage Susceptibility in Relation to Antibiotic Resistance |
| title_full_unstemmed | <i>Staphylococcus aureus</i> Isolated from the Oral Cavity: Phage Susceptibility in Relation to Antibiotic Resistance |
| title_short | <i>Staphylococcus aureus</i> Isolated from the Oral Cavity: Phage Susceptibility in Relation to Antibiotic Resistance |
| title_sort | i staphylococcus aureus i isolated from the oral cavity phage susceptibility in relation to antibiotic resistance |
| topic | bacteriophage phage therapy <i>Staphylococcus aureus</i> oral cavity antibiotic resistance MRSA |
| url | https://www.mdpi.com/2079-6382/10/11/1329 |
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