Determination of riboflavin in vitamin tablets by ion chromatography with post-column photochemical derivatization and fluorescence detection(离子色谱-柱后光化学衍生荧光检测法测定保健品中的核黄素)
采用离子色谱(IC)和光化学衍生荧光检测(PCF)联用技术，建立了保健品中核黄素的分析方法.色谱柱选用Dionex Ion Pac AG11-HC保护柱(4 mm×50 mm)和Ion Pac AS11-HC分析柱(4 mm × 250 mm)，淋洗液为40 mmol• L-1 NaOH.待测物通过自制光化学反应器(254 nm)在线衍生，核黄素转化为强荧光物质，从而以荧光检测器检测.在优化的实验条件下，对50 mg • L-1的核黄素溶液平行测定6次的保留时间和峰面积相对标准偏差分别为0.6%和2.1%(n = 6).核黄素的浓度在10~100 mg• L-1范围内与峰面积呈现良好的线性关系...
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| Format: | Article |
| Language: | zho |
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Zhejiang University Press
2013-05-01
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| Series: | Zhejiang Daxue xuebao. Lixue ban |
| Subjects: | |
| Online Access: | https://doi.org/10.3785/j.issn.1008-9497.2013.03.013 |
| _version_ | 1797235876086939648 |
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| author | CAOSheng-lin(曹盛林) CHENYu(陈郁) QAMARSubhani() ZHUYan(朱岩) LÜWei-de(吕伟德) |
| author_facet | CAOSheng-lin(曹盛林) CHENYu(陈郁) QAMARSubhani() ZHUYan(朱岩) LÜWei-de(吕伟德) |
| author_sort | CAOSheng-lin(曹盛林) |
| collection | DOAJ |
| description | 采用离子色谱(IC)和光化学衍生荧光检测(PCF)联用技术，建立了保健品中核黄素的分析方法.色谱柱选用Dionex Ion Pac AG11-HC保护柱(4 mm×50 mm)和Ion Pac AS11-HC分析柱(4 mm × 250 mm)，淋洗液为40 mmol• L-1 NaOH.待测物通过自制光化学反应器(254 nm)在线衍生，核黄素转化为强荧光物质，从而以荧光检测器检测.在优化的实验条件下，对50 mg • L-1的核黄素溶液平行测定6次的保留时间和峰面积相对标准偏差分别为0.6%和2.1%(n = 6).核黄素的浓度在10~100 mg• L-1范围内与峰面积呈现良好的线性关系，线性相关系数r=0.993，检出限(S/N=10)为0.5 mg• L-1，回收率为(101.46±2.51)%. |
| first_indexed | 2024-04-24T16:54:55Z |
| format | Article |
| id | doaj.art-16eac2cc7c4d4b109ccabeffd96dd7d1 |
| institution | Directory Open Access Journal |
| issn | 1008-9497 |
| language | zho |
| last_indexed | 2024-04-24T16:54:55Z |
| publishDate | 2013-05-01 |
| publisher | Zhejiang University Press |
| record_format | Article |
| series | Zhejiang Daxue xuebao. Lixue ban |
| spelling | doaj.art-16eac2cc7c4d4b109ccabeffd96dd7d12024-03-29T01:58:32ZzhoZhejiang University PressZhejiang Daxue xuebao. Lixue ban1008-94972013-05-0140330430710.3785/j.issn.1008-9497.2013.03.013Determination of riboflavin in vitamin tablets by ion chromatography with post-column photochemical derivatization and fluorescence detection(离子色谱-柱后光化学衍生荧光检测法测定保健品中的核黄素)CAOSheng-lin(曹盛林)0CHENYu(陈郁)1QAMARSubhani()2ZHUYan(朱岩)3LÜWei-de(吕伟德)4 1. Department of Chemistry, Zhejiang University, Hangzhou 310028, China( 1.浙江大学 化学系，浙江 杭州 310028) 2. Hangzhou Vocational and Technical College, Hangzhou 310018, China( 2.杭州职业技术学院，浙江 杭州 310018) 1. Department of Chemistry, Zhejiang University, Hangzhou 310028, China( 1.浙江大学 化学系，浙江 杭州 310028) 1. Department of Chemistry, Zhejiang University, Hangzhou 310028, China( 1.浙江大学 化学系，浙江 杭州 310028) 2. Hangzhou Vocational and Technical College, Hangzhou 310018, China( 2.杭州职业技术学院，浙江 杭州 310018)采用离子色谱(IC)和光化学衍生荧光检测(PCF)联用技术，建立了保健品中核黄素的分析方法.色谱柱选用Dionex Ion Pac AG11-HC保护柱(4 mm×50 mm)和Ion Pac AS11-HC分析柱(4 mm × 250 mm)，淋洗液为40 mmol• L-1 NaOH.待测物通过自制光化学反应器(254 nm)在线衍生，核黄素转化为强荧光物质，从而以荧光检测器检测.在优化的实验条件下，对50 mg • L-1的核黄素溶液平行测定6次的保留时间和峰面积相对标准偏差分别为0.6%和2.1%(n = 6).核黄素的浓度在10~100 mg• L-1范围内与峰面积呈现良好的线性关系，线性相关系数r=0.993，检出限(S/N=10)为0.5 mg• L-1，回收率为(101.46±2.51)%.https://doi.org/10.3785/j.issn.1008-9497.2013.03.013离子色谱柱后光化学衍生荧光检测核黄素 |
| spellingShingle | CAOSheng-lin(曹盛林) CHENYu(陈郁) QAMARSubhani() ZHUYan(朱岩) LÜWei-de(吕伟德) Determination of riboflavin in vitamin tablets by ion chromatography with post-column photochemical derivatization and fluorescence detection(离子色谱-柱后光化学衍生荧光检测法测定保健品中的核黄素) Zhejiang Daxue xuebao. Lixue ban 离子色谱 柱后光化学衍生 荧光检测 核黄素 |
| title | Determination of riboflavin in vitamin tablets by ion chromatography with post-column photochemical derivatization and fluorescence detection(离子色谱-柱后光化学衍生荧光检测法测定保健品中的核黄素) |
| title_full | Determination of riboflavin in vitamin tablets by ion chromatography with post-column photochemical derivatization and fluorescence detection(离子色谱-柱后光化学衍生荧光检测法测定保健品中的核黄素) |
| title_fullStr | Determination of riboflavin in vitamin tablets by ion chromatography with post-column photochemical derivatization and fluorescence detection(离子色谱-柱后光化学衍生荧光检测法测定保健品中的核黄素) |
| title_full_unstemmed | Determination of riboflavin in vitamin tablets by ion chromatography with post-column photochemical derivatization and fluorescence detection(离子色谱-柱后光化学衍生荧光检测法测定保健品中的核黄素) |
| title_short | Determination of riboflavin in vitamin tablets by ion chromatography with post-column photochemical derivatization and fluorescence detection(离子色谱-柱后光化学衍生荧光检测法测定保健品中的核黄素) |
| title_sort | determination of riboflavin in vitamin tablets by ion chromatography with post column photochemical derivatization and fluorescence detection 离子色谱 柱后光化学衍生荧光检测法测定保健品中的核黄素 |
| topic | 离子色谱 柱后光化学衍生 荧光检测 核黄素 |
| url | https://doi.org/10.3785/j.issn.1008-9497.2013.03.013 |
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