Neuroprotective effects of tenuigenin on neurobehavior, oxidative stress, and tau hyperphosphorylation induced by intracerebroventricular streptozotocin in rats
BACKGROUND: Tenuigenin (TEN), a major active component of the Chinese herb Polygala tenuifolia root, has been used to improve memory and cognitive function in Traditional Chinese Medicine for centuries. PURPOSE: The present study was designed to explore the possible neuroprotective effect of TEN on...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wolters Kluwer Medknow Publications
2018-01-01
|
| Series: | Brain Circulation |
| Subjects: | |
| Online Access: | http://www.braincirculation.org/article.asp?issn=2394-8108;year=2018;volume=4;issue=1;spage=24;epage=32;aulast=Huang |
| _version_ | 1828374295501340672 |
|---|---|
| author | Xiao-Bo Huang Yu-Jing Chen Wen-Qiang Chen Ning-Qun Wang Xi-Ling Wu Yan Liu |
| author_facet | Xiao-Bo Huang Yu-Jing Chen Wen-Qiang Chen Ning-Qun Wang Xi-Ling Wu Yan Liu |
| author_sort | Xiao-Bo Huang |
| collection | DOAJ |
| description | BACKGROUND: Tenuigenin (TEN), a major active component of the Chinese herb Polygala tenuifolia root, has been used to improve memory and cognitive function in Traditional Chinese Medicine for centuries. PURPOSE: The present study was designed to explore the possible neuroprotective effect of TEN on the streptozotocin (STZ)-induced rat model of sporadic Alzheimer's disease (sAD). METHODS: STZ was injected twice intracerebroventrically (3 mg/kg, ICV) on alternate days (day 1 and day 3) in Rats. Daily treatment with TEN (2, 4, and 8 mg/kg) starting from the first dose of STZ for 28 days. Memory-related behaviors were evaluated using the Morris water maze test. Hyperphosphorylation of tau proteins in hippocampus were measured by western blot assay. Superoxide dismutase activities, malondialdehyde, glutathione peroxidase and 4-hydroxy-2-nonenal adducts contents were also measured in the hippocampus.RESULTS: Treatment with TEN significantly improved STZ-induced cognitive damage, markedly reduced changes in malondialdehyde and 4-hydroxy-2-nonenal adducts, and significantly inhibited STZ-induced reduction in superoxide dismutase and glutathione peroxidase activities in the hippocampus. In addition, TEN decreased hyperphosphorylation of tau resulting from intracerebroventricular STZ (ICV-STZ) injection, and Nissl staining results showed that TEN has protective effects on hippocampal neurons. CONCLUSION: These results provide experimental evidence demonstrating preventive effect of TEN on cognitive dysfunction, oxidative stress, and hyperphosphorylation of tau in ICV-STZ rats. This study indicates that TEN may have beneficial effects in the treatment of neurodegenerative disorders such as AD. |
| first_indexed | 2024-04-14T07:31:41Z |
| format | Article |
| id | doaj.art-16f0dfd97ffe4affbb93a313fed11a26 |
| institution | Directory Open Access Journal |
| issn | 2394-8108 2455-4626 |
| language | English |
| last_indexed | 2024-04-14T07:31:41Z |
| publishDate | 2018-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Brain Circulation |
| spelling | doaj.art-16f0dfd97ffe4affbb93a313fed11a262022-12-22T02:05:50ZengWolters Kluwer Medknow PublicationsBrain Circulation2394-81082455-46262018-01-0141243210.4103/bc.BC_2_17Neuroprotective effects of tenuigenin on neurobehavior, oxidative stress, and tau hyperphosphorylation induced by intracerebroventricular streptozotocin in ratsXiao-Bo HuangYu-Jing ChenWen-Qiang ChenNing-Qun WangXi-Ling WuYan LiuBACKGROUND: Tenuigenin (TEN), a major active component of the Chinese herb Polygala tenuifolia root, has been used to improve memory and cognitive function in Traditional Chinese Medicine for centuries. PURPOSE: The present study was designed to explore the possible neuroprotective effect of TEN on the streptozotocin (STZ)-induced rat model of sporadic Alzheimer's disease (sAD). METHODS: STZ was injected twice intracerebroventrically (3 mg/kg, ICV) on alternate days (day 1 and day 3) in Rats. Daily treatment with TEN (2, 4, and 8 mg/kg) starting from the first dose of STZ for 28 days. Memory-related behaviors were evaluated using the Morris water maze test. Hyperphosphorylation of tau proteins in hippocampus were measured by western blot assay. Superoxide dismutase activities, malondialdehyde, glutathione peroxidase and 4-hydroxy-2-nonenal adducts contents were also measured in the hippocampus.RESULTS: Treatment with TEN significantly improved STZ-induced cognitive damage, markedly reduced changes in malondialdehyde and 4-hydroxy-2-nonenal adducts, and significantly inhibited STZ-induced reduction in superoxide dismutase and glutathione peroxidase activities in the hippocampus. In addition, TEN decreased hyperphosphorylation of tau resulting from intracerebroventricular STZ (ICV-STZ) injection, and Nissl staining results showed that TEN has protective effects on hippocampal neurons. CONCLUSION: These results provide experimental evidence demonstrating preventive effect of TEN on cognitive dysfunction, oxidative stress, and hyperphosphorylation of tau in ICV-STZ rats. This study indicates that TEN may have beneficial effects in the treatment of neurodegenerative disorders such as AD.http://www.braincirculation.org/article.asp?issn=2394-8108;year=2018;volume=4;issue=1;spage=24;epage=32;aulast=HuangIntracerebroventricular streptozotocinoxidative stresstau hyperphosphorylationtenuigenin |
| spellingShingle | Xiao-Bo Huang Yu-Jing Chen Wen-Qiang Chen Ning-Qun Wang Xi-Ling Wu Yan Liu Neuroprotective effects of tenuigenin on neurobehavior, oxidative stress, and tau hyperphosphorylation induced by intracerebroventricular streptozotocin in rats Brain Circulation Intracerebroventricular streptozotocin oxidative stress tau hyperphosphorylation tenuigenin |
| title | Neuroprotective effects of tenuigenin on neurobehavior, oxidative stress, and tau hyperphosphorylation induced by intracerebroventricular streptozotocin in rats |
| title_full | Neuroprotective effects of tenuigenin on neurobehavior, oxidative stress, and tau hyperphosphorylation induced by intracerebroventricular streptozotocin in rats |
| title_fullStr | Neuroprotective effects of tenuigenin on neurobehavior, oxidative stress, and tau hyperphosphorylation induced by intracerebroventricular streptozotocin in rats |
| title_full_unstemmed | Neuroprotective effects of tenuigenin on neurobehavior, oxidative stress, and tau hyperphosphorylation induced by intracerebroventricular streptozotocin in rats |
| title_short | Neuroprotective effects of tenuigenin on neurobehavior, oxidative stress, and tau hyperphosphorylation induced by intracerebroventricular streptozotocin in rats |
| title_sort | neuroprotective effects of tenuigenin on neurobehavior oxidative stress and tau hyperphosphorylation induced by intracerebroventricular streptozotocin in rats |
| topic | Intracerebroventricular streptozotocin oxidative stress tau hyperphosphorylation tenuigenin |
| url | http://www.braincirculation.org/article.asp?issn=2394-8108;year=2018;volume=4;issue=1;spage=24;epage=32;aulast=Huang |
| work_keys_str_mv | AT xiaobohuang neuroprotectiveeffectsoftenuigeninonneurobehavioroxidativestressandtauhyperphosphorylationinducedbyintracerebroventricularstreptozotocininrats AT yujingchen neuroprotectiveeffectsoftenuigeninonneurobehavioroxidativestressandtauhyperphosphorylationinducedbyintracerebroventricularstreptozotocininrats AT wenqiangchen neuroprotectiveeffectsoftenuigeninonneurobehavioroxidativestressandtauhyperphosphorylationinducedbyintracerebroventricularstreptozotocininrats AT ningqunwang neuroprotectiveeffectsoftenuigeninonneurobehavioroxidativestressandtauhyperphosphorylationinducedbyintracerebroventricularstreptozotocininrats AT xilingwu neuroprotectiveeffectsoftenuigeninonneurobehavioroxidativestressandtauhyperphosphorylationinducedbyintracerebroventricularstreptozotocininrats AT yanliu neuroprotectiveeffectsoftenuigeninonneurobehavioroxidativestressandtauhyperphosphorylationinducedbyintracerebroventricularstreptozotocininrats |