Current Methods, Common Practices, and Perspectives in Tracking and Monitoring Bioinoculants in Soil
Microorganisms promised to lead the bio-based revolution for a more sustainable agriculture. Beneficial microorganisms could be a valid alternative to the use of chemical fertilizers or pesticides. However, the increasing use of microbial inoculants is also raising several questions about their effi...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2021-08-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2021.698491/full |
| _version_ | 1818642093349797888 |
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| author | Andrea Manfredini Eligio Malusà Eligio Malusà Corrado Costa Federico Pallottino Stefano Mocali Flavia Pinzari Flavia Pinzari Loredana Canfora |
| author_facet | Andrea Manfredini Eligio Malusà Eligio Malusà Corrado Costa Federico Pallottino Stefano Mocali Flavia Pinzari Flavia Pinzari Loredana Canfora |
| author_sort | Andrea Manfredini |
| collection | DOAJ |
| description | Microorganisms promised to lead the bio-based revolution for a more sustainable agriculture. Beneficial microorganisms could be a valid alternative to the use of chemical fertilizers or pesticides. However, the increasing use of microbial inoculants is also raising several questions about their efficacy and their effects on the autochthonous soil microorganisms. There are two major issues on the application of bioinoculants to soil: (i) their detection in soil, and the analysis of their persistence and fate; (ii) the monitoring of the impact of the introduced bioinoculant on native soil microbial communities. This review explores the strategies and methods that can be applied to the detection of microbial inoculants and to soil monitoring. The discussion includes a comprehensive critical assessment of the available tools, based on morpho-phenological, molecular, and microscopic analyses. The prospects for future development of protocols for regulatory or commercial purposes are also discussed, underlining the need for a multi-method (polyphasic) approach to ensure the necessary level of discrimination required to track and monitor bioinoculants in soil. |
| first_indexed | 2024-12-16T23:37:35Z |
| format | Article |
| id | doaj.art-16f0ef077fa2414380d1cf9e94261c93 |
| institution | Directory Open Access Journal |
| issn | 1664-302X |
| language | English |
| last_indexed | 2024-12-16T23:37:35Z |
| publishDate | 2021-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Microbiology |
| spelling | doaj.art-16f0ef077fa2414380d1cf9e94261c932022-12-21T22:11:43ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2021-08-011210.3389/fmicb.2021.698491698491Current Methods, Common Practices, and Perspectives in Tracking and Monitoring Bioinoculants in SoilAndrea Manfredini0Eligio Malusà1Eligio Malusà2Corrado Costa3Federico Pallottino4Stefano Mocali5Flavia Pinzari6Flavia Pinzari7Loredana Canfora8Council for Agricultural Research and Economics, Research Centre for Agriculture and Environment, Rome, ItalyNational Research Institute of Horticulture, Skierniewice, PolandCouncil for Agricultural Research and Economics, Research Centre for Viticulture and Enology, Conegliano, ItalyCouncil for Agricultural Research and Analysis of the Agricultural Economy, Research Centre for Engineering and Agro-Food Processing, Monterotondo, ItalyCouncil for Agricultural Research and Analysis of the Agricultural Economy, Research Centre for Engineering and Agro-Food Processing, Monterotondo, ItalyCouncil for Agricultural Research and Economics, Research Centre for Agriculture and Environment, Rome, ItalyInstitute for Biological Systems, Council of National Research of Italy (CNR), Rome, ItalyLife Sciences Department, Natural History Museum, London, United KingdomCouncil for Agricultural Research and Economics, Research Centre for Agriculture and Environment, Rome, ItalyMicroorganisms promised to lead the bio-based revolution for a more sustainable agriculture. Beneficial microorganisms could be a valid alternative to the use of chemical fertilizers or pesticides. However, the increasing use of microbial inoculants is also raising several questions about their efficacy and their effects on the autochthonous soil microorganisms. There are two major issues on the application of bioinoculants to soil: (i) their detection in soil, and the analysis of their persistence and fate; (ii) the monitoring of the impact of the introduced bioinoculant on native soil microbial communities. This review explores the strategies and methods that can be applied to the detection of microbial inoculants and to soil monitoring. The discussion includes a comprehensive critical assessment of the available tools, based on morpho-phenological, molecular, and microscopic analyses. The prospects for future development of protocols for regulatory or commercial purposes are also discussed, underlining the need for a multi-method (polyphasic) approach to ensure the necessary level of discrimination required to track and monitor bioinoculants in soil.https://www.frontiersin.org/articles/10.3389/fmicb.2021.698491/fullsoildetectionmicrobial inoculantsbacteriafungibiofertilisers |
| spellingShingle | Andrea Manfredini Eligio Malusà Eligio Malusà Corrado Costa Federico Pallottino Stefano Mocali Flavia Pinzari Flavia Pinzari Loredana Canfora Current Methods, Common Practices, and Perspectives in Tracking and Monitoring Bioinoculants in Soil Frontiers in Microbiology soil detection microbial inoculants bacteria fungi biofertilisers |
| title | Current Methods, Common Practices, and Perspectives in Tracking and Monitoring Bioinoculants in Soil |
| title_full | Current Methods, Common Practices, and Perspectives in Tracking and Monitoring Bioinoculants in Soil |
| title_fullStr | Current Methods, Common Practices, and Perspectives in Tracking and Monitoring Bioinoculants in Soil |
| title_full_unstemmed | Current Methods, Common Practices, and Perspectives in Tracking and Monitoring Bioinoculants in Soil |
| title_short | Current Methods, Common Practices, and Perspectives in Tracking and Monitoring Bioinoculants in Soil |
| title_sort | current methods common practices and perspectives in tracking and monitoring bioinoculants in soil |
| topic | soil detection microbial inoculants bacteria fungi biofertilisers |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2021.698491/full |
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