Terpenoid Biosynthesis Dominates among Secondary Metabolite Clusters in <i>Mucoromycotina</i> Genomes
Early-diverging fungi harbour unprecedented diversity in terms of living forms, biological traits and genome architecture. Before the sequencing era, non-Dikarya fungi were considered unable to produce secondary metabolites (SM); however, this perspective is changing. The main classes of secondary m...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2021-04-01
|
| Series: | Journal of Fungi |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2309-608X/7/4/285 |
| _version_ | 1827695466625630208 |
|---|---|
| author | Grzegorz Koczyk Julia Pawłowska Anna Muszewska |
| author_facet | Grzegorz Koczyk Julia Pawłowska Anna Muszewska |
| author_sort | Grzegorz Koczyk |
| collection | DOAJ |
| description | Early-diverging fungi harbour unprecedented diversity in terms of living forms, biological traits and genome architecture. Before the sequencing era, non-Dikarya fungi were considered unable to produce secondary metabolites (SM); however, this perspective is changing. The main classes of secondary metabolites in fungi include polyketides, nonribosomal peptides, terpenoids and siderophores that serve different biological roles, including iron chelation and plant growth promotion. The same classes of SM are reported for representatives of early-diverging fungal lineages. Encouraged by the advancement in the field, we carried out a systematic survey of SM in <i>Mucoromycotina</i> and corroborated the presence of various SM clusters (SMCs) within the phylum. Among the core findings, considerable representation of terpene and nonribosomal peptide synthetase (NRPS)-like candidate SMCs was found. Terpene clusters with diverse domain composition and potentially highly variable products dominated the landscape of candidate SMCs. A uniform low-copy distribution of siderophore clusters was observed among most assemblies. <i>Mortierellomycotina</i> are highlighted as the most potent SMC producers among the <i>Mucoromycota</i> and as a source of novel peptide products. SMC identification is dependent on gene model quality and can be successfully performed on a batch scale with genomes of different quality and completeness. |
| first_indexed | 2024-03-10T12:27:41Z |
| format | Article |
| id | doaj.art-16f2e3f9fad34f80ba57c4fdb814d81a |
| institution | Directory Open Access Journal |
| issn | 2309-608X |
| language | English |
| last_indexed | 2024-03-10T12:27:41Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Journal of Fungi |
| spelling | doaj.art-16f2e3f9fad34f80ba57c4fdb814d81a2023-11-21T14:53:07ZengMDPI AGJournal of Fungi2309-608X2021-04-017428510.3390/jof7040285Terpenoid Biosynthesis Dominates among Secondary Metabolite Clusters in <i>Mucoromycotina</i> GenomesGrzegorz Koczyk0Julia Pawłowska1Anna Muszewska2Department of Biometry and Bioinformatics, Institute of Plant Genetics, Polish Academy of Sciences, Strzeszynska 34, 60-479 Poznan, PolandInstitute of Evolutionary Biology, Faculty of Biology, Biological and Chemical Research Centre, University of Warsaw, Zwirki i Wigury 101, 02-089 Warsaw, PolandInstitute of Biochemistry and Biophysics, Polish Academy of Sciences, Pawinskiego 5A, 02-106 Warsaw, PolandEarly-diverging fungi harbour unprecedented diversity in terms of living forms, biological traits and genome architecture. Before the sequencing era, non-Dikarya fungi were considered unable to produce secondary metabolites (SM); however, this perspective is changing. The main classes of secondary metabolites in fungi include polyketides, nonribosomal peptides, terpenoids and siderophores that serve different biological roles, including iron chelation and plant growth promotion. The same classes of SM are reported for representatives of early-diverging fungal lineages. Encouraged by the advancement in the field, we carried out a systematic survey of SM in <i>Mucoromycotina</i> and corroborated the presence of various SM clusters (SMCs) within the phylum. Among the core findings, considerable representation of terpene and nonribosomal peptide synthetase (NRPS)-like candidate SMCs was found. Terpene clusters with diverse domain composition and potentially highly variable products dominated the landscape of candidate SMCs. A uniform low-copy distribution of siderophore clusters was observed among most assemblies. <i>Mortierellomycotina</i> are highlighted as the most potent SMC producers among the <i>Mucoromycota</i> and as a source of novel peptide products. SMC identification is dependent on gene model quality and can be successfully performed on a batch scale with genomes of different quality and completeness.https://www.mdpi.com/2309-608X/7/4/285<i>Mucoromycotina</i>secondary metabolitenatural productsSM biosynthesisterpenesNRPS |
| spellingShingle | Grzegorz Koczyk Julia Pawłowska Anna Muszewska Terpenoid Biosynthesis Dominates among Secondary Metabolite Clusters in <i>Mucoromycotina</i> Genomes Journal of Fungi <i>Mucoromycotina</i> secondary metabolite natural products SM biosynthesis terpenes NRPS |
| title | Terpenoid Biosynthesis Dominates among Secondary Metabolite Clusters in <i>Mucoromycotina</i> Genomes |
| title_full | Terpenoid Biosynthesis Dominates among Secondary Metabolite Clusters in <i>Mucoromycotina</i> Genomes |
| title_fullStr | Terpenoid Biosynthesis Dominates among Secondary Metabolite Clusters in <i>Mucoromycotina</i> Genomes |
| title_full_unstemmed | Terpenoid Biosynthesis Dominates among Secondary Metabolite Clusters in <i>Mucoromycotina</i> Genomes |
| title_short | Terpenoid Biosynthesis Dominates among Secondary Metabolite Clusters in <i>Mucoromycotina</i> Genomes |
| title_sort | terpenoid biosynthesis dominates among secondary metabolite clusters in i mucoromycotina i genomes |
| topic | <i>Mucoromycotina</i> secondary metabolite natural products SM biosynthesis terpenes NRPS |
| url | https://www.mdpi.com/2309-608X/7/4/285 |
| work_keys_str_mv | AT grzegorzkoczyk terpenoidbiosynthesisdominatesamongsecondarymetaboliteclustersinimucoromycotinaigenomes AT juliapawłowska terpenoidbiosynthesisdominatesamongsecondarymetaboliteclustersinimucoromycotinaigenomes AT annamuszewska terpenoidbiosynthesisdominatesamongsecondarymetaboliteclustersinimucoromycotinaigenomes |