Oxygen availability strongly affects chronological lifespan and thermotolerance in batch cultures of Saccharomyces cerevisiae
Stationary-phase (SP) batch cultures of Saccharomyces cerevisiae, in which growth has been arrested by carbon-source depletion, are widely applied to study chronological lifespan, quiescence and SP-associated robustness. Based on this type of experiments, typically performed under aerobic c...
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Shared Science Publishers OG
2015-10-01
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| Series: | Microbial Cell |
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| Online Access: | http://microbialcell.com/researcharticles/oxygen-availability-strongly-affects-chronological-lifespan-and-thermotolerance-in-batch-cultures-of-saccharomyces-cerevisiae/ |
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| author | Markus M.M. Bisschops Tim Vos Rubén Martínez-Moreno Pilar de la Torre Cortés Jack T. Pronk Pascale Daran-Lapujade |
| author_facet | Markus M.M. Bisschops Tim Vos Rubén Martínez-Moreno Pilar de la Torre Cortés Jack T. Pronk Pascale Daran-Lapujade |
| author_sort | Markus M.M. Bisschops |
| collection | DOAJ |
| description | Stationary-phase (SP) batch cultures of Saccharomyces cerevisiae, in which growth has been arrested by carbon-source depletion, are widely applied to study chronological lifespan, quiescence and SP-associated robustness. Based on this type of experiments, typically performed under aerobic conditions, several roles of oxygen in aging have been proposed. However, SP in anaerobic yeast cultures has not been investigated in detail. Here, we use the unique capability of S. cerevisiae to grow in the complete absence of oxygen to directly compare SP in aerobic and anaerobic bioreactor cultures. This comparison revealed strong positive effects of oxygen availability on adenylate energy charge, longevity and thermotolerance during SP. A low thermotolerance of anaerobic batch cultures was already evident during the exponential growth phase and, in contrast to the situation in aerobic cultures, was not substantially increased during transition into SP. A combination of physiological and transcriptome analysis showed that the slow post-diauxic growth phase on ethanol, which precedes SP in aerobic, but not in anaerobic cultures, endowed cells with the time and resources needed for inducing longevity and thermotolerance. When combined with literature data on acquisition of longevity and thermotolerance in retentostat cultures, the present study indicates that the fast transition from glucose excess to SP in anaerobic cultures precludes acquisition of longevity and thermotolerance. Moreover, this study demonstrates the importance of a preceding, calorie-restricted conditioning phase in the acquisition of longevity and stress tolerance in SP yeast cultures, irrespective of oxygen availability. |
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| institution | Directory Open Access Journal |
| issn | 2311-2638 |
| language | English |
| last_indexed | 2025-03-14T01:44:32Z |
| publishDate | 2015-10-01 |
| publisher | Shared Science Publishers OG |
| record_format | Article |
| series | Microbial Cell |
| spelling | doaj.art-16f30b037e9a441ea8bea4bda9b7ffff2025-03-12T13:46:44ZengShared Science Publishers OGMicrobial Cell2311-26382015-10-0121142944410.15698/mic2015.11.238123455678Oxygen availability strongly affects chronological lifespan and thermotolerance in batch cultures of Saccharomyces cerevisiaeMarkus M.M. Bisschops0Tim Vos1Rubén Martínez-Moreno2Pilar de la Torre Cortés3Jack T. Pronk4Pascale Daran-Lapujade5Department of Biotechnology, Delft University of Technology, Delft, The Netherlands.Department of Biotechnology, Delft University of Technology, Delft, The Netherlands.Instituto de Ciencias de la Vid y del Vino, CSIC, Universidad de La Rioja, Gobierno de La Rioja, Logroño, Spain.Department of Biotechnology, Delft University of Technology, Delft, The Netherlands.Department of Biotechnology, Delft University of Technology, Delft, The Netherlands.Department of Biotechnology, Delft University of Technology, Delft, The Netherlands.Stationary-phase (SP) batch cultures of Saccharomyces cerevisiae, in which growth has been arrested by carbon-source depletion, are widely applied to study chronological lifespan, quiescence and SP-associated robustness. Based on this type of experiments, typically performed under aerobic conditions, several roles of oxygen in aging have been proposed. However, SP in anaerobic yeast cultures has not been investigated in detail. Here, we use the unique capability of S. cerevisiae to grow in the complete absence of oxygen to directly compare SP in aerobic and anaerobic bioreactor cultures. This comparison revealed strong positive effects of oxygen availability on adenylate energy charge, longevity and thermotolerance during SP. A low thermotolerance of anaerobic batch cultures was already evident during the exponential growth phase and, in contrast to the situation in aerobic cultures, was not substantially increased during transition into SP. A combination of physiological and transcriptome analysis showed that the slow post-diauxic growth phase on ethanol, which precedes SP in aerobic, but not in anaerobic cultures, endowed cells with the time and resources needed for inducing longevity and thermotolerance. When combined with literature data on acquisition of longevity and thermotolerance in retentostat cultures, the present study indicates that the fast transition from glucose excess to SP in anaerobic cultures precludes acquisition of longevity and thermotolerance. Moreover, this study demonstrates the importance of a preceding, calorie-restricted conditioning phase in the acquisition of longevity and stress tolerance in SP yeast cultures, irrespective of oxygen availability.http://microbialcell.com/researcharticles/oxygen-availability-strongly-affects-chronological-lifespan-and-thermotolerance-in-batch-cultures-of-saccharomyces-cerevisiae/chronological lifespanthermotolerancestationary phaseanaerobiosisenergeticstranscriptional responseconditioning |
| spellingShingle | Markus M.M. Bisschops Tim Vos Rubén Martínez-Moreno Pilar de la Torre Cortés Jack T. Pronk Pascale Daran-Lapujade Oxygen availability strongly affects chronological lifespan and thermotolerance in batch cultures of Saccharomyces cerevisiae Microbial Cell chronological lifespan thermotolerance stationary phase anaerobiosis energetics transcriptional response conditioning |
| title | Oxygen availability strongly affects chronological lifespan and thermotolerance in batch cultures of Saccharomyces cerevisiae |
| title_full | Oxygen availability strongly affects chronological lifespan and thermotolerance in batch cultures of Saccharomyces cerevisiae |
| title_fullStr | Oxygen availability strongly affects chronological lifespan and thermotolerance in batch cultures of Saccharomyces cerevisiae |
| title_full_unstemmed | Oxygen availability strongly affects chronological lifespan and thermotolerance in batch cultures of Saccharomyces cerevisiae |
| title_short | Oxygen availability strongly affects chronological lifespan and thermotolerance in batch cultures of Saccharomyces cerevisiae |
| title_sort | oxygen availability strongly affects chronological lifespan and thermotolerance in batch cultures of saccharomyces cerevisiae |
| topic | chronological lifespan thermotolerance stationary phase anaerobiosis energetics transcriptional response conditioning |
| url | http://microbialcell.com/researcharticles/oxygen-availability-strongly-affects-chronological-lifespan-and-thermotolerance-in-batch-cultures-of-saccharomyces-cerevisiae/ |
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