Detection of Fur, AmoA and pvcAB genes in Aeromonas hydrophila isolated from aquatic organisms and impact on bacterial growth under different iron concentrations
ABSTRACT Infection caused by Aeromonas brings great harm to fish farming. Among the factors associated with bacterial pathogenesis, iron uptake can contribute to the survival and virulence of bacteria within hosts. The aim of this study was to check the presence of genes related to iron uptake in Ae...
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| Format: | Article |
| Language: | English |
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Universidade Federal de Minas Gerais
2022-08-01
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| Series: | Arquivo Brasileiro de Medicina Veterinária e Zootecnia |
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| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352022000400671&tlng=en |
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| author | M.R. Freitas B.N. Araujo R.A.N. Soares J.J.S. Gouveia M.M. Costa G.V. Gouveia |
| author_facet | M.R. Freitas B.N. Araujo R.A.N. Soares J.J.S. Gouveia M.M. Costa G.V. Gouveia |
| author_sort | M.R. Freitas |
| collection | DOAJ |
| description | ABSTRACT Infection caused by Aeromonas brings great harm to fish farming. Among the factors associated with bacterial pathogenesis, iron uptake can contribute to the survival and virulence of bacteria within hosts. The aim of this study was to check the presence of genes related to iron uptake in Aeromonas hydrophila deriving from aquatic organisms in the São Francisco Valley and associate the presence of these genes with the ability to grow in media containing different concentrations of iron. The DNAs of 41 isolates were extracted and used in PCRs to verify the presence of the Fur, AmoA and pvcAB genes related to iron uptake. The growth of the isolates belonging to different genetic profiles was verified in culture media containing different iron concentrations. Two isolates were positive for the presence of the Fur gene, seven for the AmoA gene and two for the pvcAB gene. The growth test showed that the low availability of iron did not interfere in the growth of the isolates, nor in the isolate that did not contain any of the genes evaluated in this study, suggesting that the iron uptake’s mechanisms of the tested isolates may be related to other genes and proteins. |
| first_indexed | 2024-04-13T02:14:31Z |
| format | Article |
| id | doaj.art-16f408d393564acd929d2da9470c27ef |
| institution | Directory Open Access Journal |
| issn | 1678-4162 |
| language | English |
| last_indexed | 2024-04-13T02:14:31Z |
| publishDate | 2022-08-01 |
| publisher | Universidade Federal de Minas Gerais |
| record_format | Article |
| series | Arquivo Brasileiro de Medicina Veterinária e Zootecnia |
| spelling | doaj.art-16f408d393564acd929d2da9470c27ef2022-12-22T03:07:11ZengUniversidade Federal de Minas GeraisArquivo Brasileiro de Medicina Veterinária e Zootecnia1678-41622022-08-0174467167610.1590/1678-4162-12491Detection of Fur, AmoA and pvcAB genes in Aeromonas hydrophila isolated from aquatic organisms and impact on bacterial growth under different iron concentrationsM.R. Freitashttps://orcid.org/0000-0002-6823-3260B.N. Araujohttps://orcid.org/0000-0003-4821-7295R.A.N. Soareshttps://orcid.org/0000-0002-4675-3978J.J.S. Gouveiahttps://orcid.org/0000-0002-0438-094XM.M. Costahttps://orcid.org/0000-0002-9884-2112G.V. Gouveiahttps://orcid.org/0000-0003-1074-5711ABSTRACT Infection caused by Aeromonas brings great harm to fish farming. Among the factors associated with bacterial pathogenesis, iron uptake can contribute to the survival and virulence of bacteria within hosts. The aim of this study was to check the presence of genes related to iron uptake in Aeromonas hydrophila deriving from aquatic organisms in the São Francisco Valley and associate the presence of these genes with the ability to grow in media containing different concentrations of iron. The DNAs of 41 isolates were extracted and used in PCRs to verify the presence of the Fur, AmoA and pvcAB genes related to iron uptake. The growth of the isolates belonging to different genetic profiles was verified in culture media containing different iron concentrations. Two isolates were positive for the presence of the Fur gene, seven for the AmoA gene and two for the pvcAB gene. The growth test showed that the low availability of iron did not interfere in the growth of the isolates, nor in the isolate that did not contain any of the genes evaluated in this study, suggesting that the iron uptake’s mechanisms of the tested isolates may be related to other genes and proteins.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352022000400671&tlng=enbacterial growthiron uptake genesfish farming |
| spellingShingle | M.R. Freitas B.N. Araujo R.A.N. Soares J.J.S. Gouveia M.M. Costa G.V. Gouveia Detection of Fur, AmoA and pvcAB genes in Aeromonas hydrophila isolated from aquatic organisms and impact on bacterial growth under different iron concentrations Arquivo Brasileiro de Medicina Veterinária e Zootecnia bacterial growth iron uptake genes fish farming |
| title | Detection of Fur, AmoA and pvcAB genes in Aeromonas hydrophila isolated from aquatic organisms and impact on bacterial growth under different iron concentrations |
| title_full | Detection of Fur, AmoA and pvcAB genes in Aeromonas hydrophila isolated from aquatic organisms and impact on bacterial growth under different iron concentrations |
| title_fullStr | Detection of Fur, AmoA and pvcAB genes in Aeromonas hydrophila isolated from aquatic organisms and impact on bacterial growth under different iron concentrations |
| title_full_unstemmed | Detection of Fur, AmoA and pvcAB genes in Aeromonas hydrophila isolated from aquatic organisms and impact on bacterial growth under different iron concentrations |
| title_short | Detection of Fur, AmoA and pvcAB genes in Aeromonas hydrophila isolated from aquatic organisms and impact on bacterial growth under different iron concentrations |
| title_sort | detection of fur amoa and pvcab genes in aeromonas hydrophila isolated from aquatic organisms and impact on bacterial growth under different iron concentrations |
| topic | bacterial growth iron uptake genes fish farming |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352022000400671&tlng=en |
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