Monocyte chemoattractant protein-1 (MCP1) -2581 A/G and interferon gamma (IFNγ) +874 T/A polymorphisms in Iranian population with pulmonary tuberculosis
Background: Genetic polymorphisms are predictors of the immune response and susceptibility to certain infectious diseases, including pulmonary tuberculosis (TB). We evaluated the association of monocyte chemoattractant protein-1 (MCP1) (-2581 A/G) and interferon-gamma (IFNγ) (+874 T/A) polymorphisms...
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| Format: | Article |
| Language: | English |
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Golestan University of Medical Sciences
2023-10-01
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| Series: | Jorjani Biomedicine Journal |
| Subjects: | |
| Online Access: | http://goums.ac.ir/jorjanijournal/article-1-986-en.pdf |
| _version_ | 1827278972482748416 |
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| author | Ailar Jamali Mojtaba Zare Ebrahimabad Sareh Zhand ayyoob khosravi |
| author_facet | Ailar Jamali Mojtaba Zare Ebrahimabad Sareh Zhand ayyoob khosravi |
| author_sort | Ailar Jamali |
| collection | DOAJ |
| description | Background: Genetic polymorphisms are predictors of the immune response and susceptibility to certain infectious diseases, including pulmonary tuberculosis (TB). We evaluated the association of monocyte chemoattractant protein-1 (MCP1) (-2581 A/G) and interferon-gamma (IFNγ) (+874 T/A) polymorphisms with susceptibility to pulmonary TB in an Iranian population.
Methods: A total of 124 patients with pulmonary tuberculosis and 244 healthy subjects (121 related normal controls and 123 unrelated subjects) were included. The MCP1 polymorphic region (-2518 A/G) was genotyped by PCR-RFLP, while ARMS-PCR was used to amplify and detect IFNγ (+874 T/A). SNPStats and SPSS v. 20 were used for the statistical analysis of the data.
Results: The comparison of MCP1 (-2518 A/G) alleles and genotypes in TB patients and healthy subjects showed no significant association in all the constructed heredity models. No association was observed between TB patients and normal subjects in all the constructed inheritance models for IFNγ (+874 T/A) alleles and genotypes.
Conclusion: Due to the lack of association between MCP1 (-2518 A/G) and IFNγ (874 T/A) polymorphisms and susceptibility to PT in our study and the conflicting results of some previous studies, further clinical and molecular research is needed to clarify the role of the studied polymorphisms in the pathogenesis of tuberculosis. |
| first_indexed | 2024-04-24T08:10:12Z |
| format | Article |
| id | doaj.art-16f6f695a9dd4763ab3b727064fbbaf8 |
| institution | Directory Open Access Journal |
| issn | 2645-3509 |
| language | English |
| last_indexed | 2024-04-24T08:10:12Z |
| publishDate | 2023-10-01 |
| publisher | Golestan University of Medical Sciences |
| record_format | Article |
| series | Jorjani Biomedicine Journal |
| spelling | doaj.art-16f6f695a9dd4763ab3b727064fbbaf82024-04-17T05:31:44ZengGolestan University of Medical SciencesJorjani Biomedicine Journal2645-35092023-10-011122427Monocyte chemoattractant protein-1 (MCP1) -2581 A/G and interferon gamma (IFNγ) +874 T/A polymorphisms in Iranian population with pulmonary tuberculosisAilar Jamali0Mojtaba Zare Ebrahimabad1Sareh Zhand2ayyoob khosravi3 Laboratory science research center, Golestan University of Medical Sciences, Gorgan, Iran Department of Biochemistry, Faculty of Medicine, Golestan University of Medical Sciences, Gorgan, Iran .Institute for Biomedical Materials and Devices, Faculty of Science, University of Technology Sydney, New South Wales 2007, Australia Department of Molecular Medicine, Faculty of Advanced Medical Technologies, Golestan University of Medical Sciences, Gorgan, Iran Background: Genetic polymorphisms are predictors of the immune response and susceptibility to certain infectious diseases, including pulmonary tuberculosis (TB). We evaluated the association of monocyte chemoattractant protein-1 (MCP1) (-2581 A/G) and interferon-gamma (IFNγ) (+874 T/A) polymorphisms with susceptibility to pulmonary TB in an Iranian population. Methods: A total of 124 patients with pulmonary tuberculosis and 244 healthy subjects (121 related normal controls and 123 unrelated subjects) were included. The MCP1 polymorphic region (-2518 A/G) was genotyped by PCR-RFLP, while ARMS-PCR was used to amplify and detect IFNγ (+874 T/A). SNPStats and SPSS v. 20 were used for the statistical analysis of the data. Results: The comparison of MCP1 (-2518 A/G) alleles and genotypes in TB patients and healthy subjects showed no significant association in all the constructed heredity models. No association was observed between TB patients and normal subjects in all the constructed inheritance models for IFNγ (+874 T/A) alleles and genotypes. Conclusion: Due to the lack of association between MCP1 (-2518 A/G) and IFNγ (874 T/A) polymorphisms and susceptibility to PT in our study and the conflicting results of some previous studies, further clinical and molecular research is needed to clarify the role of the studied polymorphisms in the pathogenesis of tuberculosis.http://goums.ac.ir/jorjanijournal/article-1-986-en.pdfmonocyte chemoattractant protein-1interferon-gammagenetic polymorphismpulmonary tuberculosis |
| spellingShingle | Ailar Jamali Mojtaba Zare Ebrahimabad Sareh Zhand ayyoob khosravi Monocyte chemoattractant protein-1 (MCP1) -2581 A/G and interferon gamma (IFNγ) +874 T/A polymorphisms in Iranian population with pulmonary tuberculosis Jorjani Biomedicine Journal monocyte chemoattractant protein-1 interferon-gamma genetic polymorphism pulmonary tuberculosis |
| title | Monocyte chemoattractant protein-1 (MCP1) -2581 A/G and interferon gamma (IFNγ) +874 T/A polymorphisms in Iranian population with pulmonary tuberculosis |
| title_full | Monocyte chemoattractant protein-1 (MCP1) -2581 A/G and interferon gamma (IFNγ) +874 T/A polymorphisms in Iranian population with pulmonary tuberculosis |
| title_fullStr | Monocyte chemoattractant protein-1 (MCP1) -2581 A/G and interferon gamma (IFNγ) +874 T/A polymorphisms in Iranian population with pulmonary tuberculosis |
| title_full_unstemmed | Monocyte chemoattractant protein-1 (MCP1) -2581 A/G and interferon gamma (IFNγ) +874 T/A polymorphisms in Iranian population with pulmonary tuberculosis |
| title_short | Monocyte chemoattractant protein-1 (MCP1) -2581 A/G and interferon gamma (IFNγ) +874 T/A polymorphisms in Iranian population with pulmonary tuberculosis |
| title_sort | monocyte chemoattractant protein 1 mcp1 2581 a g and interferon gamma ifnγ 874 t a polymorphisms in iranian population with pulmonary tuberculosis |
| topic | monocyte chemoattractant protein-1 interferon-gamma genetic polymorphism pulmonary tuberculosis |
| url | http://goums.ac.ir/jorjanijournal/article-1-986-en.pdf |
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