A New Approach for Wounding Research: MYC2 Gene Expression and Protein Stability in Wounded <i>Arabidopsis</i> Protoplasts
Wounding is a constant threat to plant survival throughout their lifespan; therefore, understanding the biological responses to wounds at the cellular level is important. The protoplast system is versatile for molecular biology, however, no wounding studies on this system have been reported. We esta...
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MDPI AG
2021-07-01
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Online Access: | https://www.mdpi.com/2223-7747/10/8/1518 |
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author | Seungmin Son Miye Kwon Jong Hee Im |
author_facet | Seungmin Son Miye Kwon Jong Hee Im |
author_sort | Seungmin Son |
collection | DOAJ |
description | Wounding is a constant threat to plant survival throughout their lifespan; therefore, understanding the biological responses to wounds at the cellular level is important. The protoplast system is versatile for molecular biology, however, no wounding studies on this system have been reported. We established a new approach for wounding research using mechanically damaged <i>Arabidopsis</i> mesophyll protoplasts. Wounded protoplasts showed typical wounding responses, such as increased MPK6 kinase activity and upregulated <i>JAZ1</i> expression. We also assessed expression profiles and protein stability of the basic helix-loop-helix transcription factor MYC2 in wounded protoplasts. Promoter activity, gene expression, and protein stability of MYC2 were compromised, but recovered in the early stage of wounding. In the late stage, the promoter activity and expression of MYC2 were increased, but the protein stability was not changed. According to the results of the present study, this new cell-based approach will be of use in various molecular studies on plant wounding. |
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language | English |
last_indexed | 2024-03-10T08:27:58Z |
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spelling | doaj.art-16fc05a77a344961ad264354e21b87cc2023-11-22T09:17:28ZengMDPI AGPlants2223-77472021-07-01108151810.3390/plants10081518A New Approach for Wounding Research: MYC2 Gene Expression and Protein Stability in Wounded <i>Arabidopsis</i> ProtoplastsSeungmin Son0Miye Kwon1Jong Hee Im2Department of Life Sciences, Korea University, 145 Anamro, Sungbuk-gu, Seoul 136701, KoreaJeju Biodiversity Research Institute (JBRI), Jeju Technopark (JTP), Jeju 63608, KoreaDepartment of Life Sciences, Korea University, 145 Anamro, Sungbuk-gu, Seoul 136701, KoreaWounding is a constant threat to plant survival throughout their lifespan; therefore, understanding the biological responses to wounds at the cellular level is important. The protoplast system is versatile for molecular biology, however, no wounding studies on this system have been reported. We established a new approach for wounding research using mechanically damaged <i>Arabidopsis</i> mesophyll protoplasts. Wounded protoplasts showed typical wounding responses, such as increased MPK6 kinase activity and upregulated <i>JAZ1</i> expression. We also assessed expression profiles and protein stability of the basic helix-loop-helix transcription factor MYC2 in wounded protoplasts. Promoter activity, gene expression, and protein stability of MYC2 were compromised, but recovered in the early stage of wounding. In the late stage, the promoter activity and expression of MYC2 were increased, but the protein stability was not changed. According to the results of the present study, this new cell-based approach will be of use in various molecular studies on plant wounding.https://www.mdpi.com/2223-7747/10/8/1518<i>Arabidopsis</i>LOX2mesophyll protoplastMYC2wounding |
spellingShingle | Seungmin Son Miye Kwon Jong Hee Im A New Approach for Wounding Research: MYC2 Gene Expression and Protein Stability in Wounded <i>Arabidopsis</i> Protoplasts Plants <i>Arabidopsis</i> LOX2 mesophyll protoplast MYC2 wounding |
title | A New Approach for Wounding Research: MYC2 Gene Expression and Protein Stability in Wounded <i>Arabidopsis</i> Protoplasts |
title_full | A New Approach for Wounding Research: MYC2 Gene Expression and Protein Stability in Wounded <i>Arabidopsis</i> Protoplasts |
title_fullStr | A New Approach for Wounding Research: MYC2 Gene Expression and Protein Stability in Wounded <i>Arabidopsis</i> Protoplasts |
title_full_unstemmed | A New Approach for Wounding Research: MYC2 Gene Expression and Protein Stability in Wounded <i>Arabidopsis</i> Protoplasts |
title_short | A New Approach for Wounding Research: MYC2 Gene Expression and Protein Stability in Wounded <i>Arabidopsis</i> Protoplasts |
title_sort | new approach for wounding research myc2 gene expression and protein stability in wounded i arabidopsis i protoplasts |
topic | <i>Arabidopsis</i> LOX2 mesophyll protoplast MYC2 wounding |
url | https://www.mdpi.com/2223-7747/10/8/1518 |
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