Polymorphism of candidate genes associated with reproductive performance in crossbred pigs
This investigation was done to analyze the polymorphism of 5 SNPs in candidate genes associated with reproductive performance in crossbred pig population by PCR-RFLP. The results revealed that out of 5 SNPs genotyped, 2 were polymorphic and 3 were monomorphic. Genotypic frequency of crossbred pigs...
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Indian Council of Agricultural Research
2020-09-01
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| Series: | Indian Journal of Animal Sciences |
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| Online Access: | https://epubs.icar.org.in/index.php/IJAnS/article/view/104637 |
| _version_ | 1797896794587267072 |
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| author | B C NAHA G K GAUR B L SAINI N R SAHOO P BORO |
| author_facet | B C NAHA G K GAUR B L SAINI N R SAHOO P BORO |
| author_sort | B C NAHA |
| collection | DOAJ |
| description |
This investigation was done to analyze the polymorphism of 5 SNPs in candidate genes associated with reproductive performance in crossbred pig population by PCR-RFLP. The results revealed that out of 5 SNPs genotyped, 2 were polymorphic and 3 were monomorphic. Genotypic frequency of crossbred pigs were CC (100%) for ESR1; GG (21%), GT 65%) and TT (14%) for Prei3; CC (100%) for FSHβ; AA (100%) for OPN and CC (21%), CT (63%) and TT (16%) for CDK20 SNPs, respectively. Allelic frequencies for these SNPs {ESR1 (c.1227C>T), Prei3 (T802G), FSHβ (c.930A>G), OPN (c.425G>A) and CDK20 (T96C)} were 1.00 (C), 0.53 and 0.47 (G/T), 1.00 (C), 1.00 (A) and 0.52 and 0.48 (C/T), respectively. The least squares analysis revealed that both polymorphic SNPs had non-significant effect on litter traits. The values of PIC, heterozygosity and allelic diversity indicated that crossbred population under investigation was of intermediate diversity for both polymorphic SNP loci.
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| first_indexed | 2024-04-10T07:48:28Z |
| format | Article |
| id | doaj.art-16fd0f782dfd4a1b8adc06a9d7a1398e |
| institution | Directory Open Access Journal |
| issn | 0367-8318 2394-3327 |
| language | English |
| last_indexed | 2024-04-10T07:48:28Z |
| publishDate | 2020-09-01 |
| publisher | Indian Council of Agricultural Research |
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| series | Indian Journal of Animal Sciences |
| spelling | doaj.art-16fd0f782dfd4a1b8adc06a9d7a1398e2023-02-23T10:24:11ZengIndian Council of Agricultural ResearchIndian Journal of Animal Sciences0367-83182394-33272020-09-0190510.56093/ijans.v90i5.104637Polymorphism of candidate genes associated with reproductive performance in crossbred pigsB C NAHA0G K GAUR1B L SAINI2N R SAHOO3P BORO4ICAR-Indian veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 IndiaICAR-Indian veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 IndiaICAR-Indian veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 IndiaICAR-Indian veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 IndiaICAR-Indian veterinary Research Institute, Izatnagar, Uttar Pradesh 243 122 India This investigation was done to analyze the polymorphism of 5 SNPs in candidate genes associated with reproductive performance in crossbred pig population by PCR-RFLP. The results revealed that out of 5 SNPs genotyped, 2 were polymorphic and 3 were monomorphic. Genotypic frequency of crossbred pigs were CC (100%) for ESR1; GG (21%), GT 65%) and TT (14%) for Prei3; CC (100%) for FSHβ; AA (100%) for OPN and CC (21%), CT (63%) and TT (16%) for CDK20 SNPs, respectively. Allelic frequencies for these SNPs {ESR1 (c.1227C>T), Prei3 (T802G), FSHβ (c.930A>G), OPN (c.425G>A) and CDK20 (T96C)} were 1.00 (C), 0.53 and 0.47 (G/T), 1.00 (C), 1.00 (A) and 0.52 and 0.48 (C/T), respectively. The least squares analysis revealed that both polymorphic SNPs had non-significant effect on litter traits. The values of PIC, heterozygosity and allelic diversity indicated that crossbred population under investigation was of intermediate diversity for both polymorphic SNP loci. https://epubs.icar.org.in/index.php/IJAnS/article/view/104637Crossbred pigLitter SizePCR-RFLPSNP |
| spellingShingle | B C NAHA G K GAUR B L SAINI N R SAHOO P BORO Polymorphism of candidate genes associated with reproductive performance in crossbred pigs Indian Journal of Animal Sciences Crossbred pig Litter Size PCR-RFLP SNP |
| title | Polymorphism of candidate genes associated with reproductive performance in crossbred pigs |
| title_full | Polymorphism of candidate genes associated with reproductive performance in crossbred pigs |
| title_fullStr | Polymorphism of candidate genes associated with reproductive performance in crossbred pigs |
| title_full_unstemmed | Polymorphism of candidate genes associated with reproductive performance in crossbred pigs |
| title_short | Polymorphism of candidate genes associated with reproductive performance in crossbred pigs |
| title_sort | polymorphism of candidate genes associated with reproductive performance in crossbred pigs |
| topic | Crossbred pig Litter Size PCR-RFLP SNP |
| url | https://epubs.icar.org.in/index.php/IJAnS/article/view/104637 |
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