Can mesenchymal stem cells and their conditioned medium assist inflammatory chondrocytes recovery?
Osteoarthritis (OA), one of the most common joint disease, affects more than 80% of the population aged 70 or over. Mesenchymal stem cells (MSCs) show multi-potent differentiation and self-renewal capability, and, after exposure to an inflammatory environment, also exhibit immunosuppressive properti...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2018-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC6248915?pdf=render |
| _version_ | 1818966801574264832 |
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| author | Yu-Chun Chen Yu-Wei Chang Kinn Poay Tan Yi-Shan Shen Yao-Horng Wang Chih-Hung Chang |
| author_facet | Yu-Chun Chen Yu-Wei Chang Kinn Poay Tan Yi-Shan Shen Yao-Horng Wang Chih-Hung Chang |
| author_sort | Yu-Chun Chen |
| collection | DOAJ |
| description | Osteoarthritis (OA), one of the most common joint disease, affects more than 80% of the population aged 70 or over. Mesenchymal stem cells (MSCs) show multi-potent differentiation and self-renewal capability, and, after exposure to an inflammatory environment, also exhibit immunosuppressive properties. In this study, we have used a model of lipopolysaccharide (LPS)-stimulated chondrocytes to evaluate MSC anti-inflammatory efficacy. The anti-inflammatory mechanism was tested in two cell-contained culture systems: (i) MSC-chondrocyte indirect contact system and (ii) MSC-chondrocyte direct contact system, and one cytokine-only culture system: MSC-conditioned medium (CM) system. Results showed that MSCs reduced chondrocyte inflammation through both paracrine secretion and cell-to-cell contact. The inflammation-associated, and free-radical-related genes were down-regulated significantly in the direct contact system on 24 h, however, the TNF-α. IL-6 were upregulated and aggrecan, COLII were downregulated on 72 h in direct contact system. Moreover, we found CM produced by MSC possess well therapeutic effect on inflammatory chondorcyte, and the 10-fold concentrated MSC-conditioned medium could down-regulated chondorcyte synthesis inflammation-associated, and free-radical-related genes, such as TNF-α, IL-1β, IL-6 and iNOS even treated for 72 h. In conclusion, MSC-CM showed great potential for MSC-based therapy for OA. |
| first_indexed | 2024-12-20T13:38:41Z |
| format | Article |
| id | doaj.art-16fd143ca144419a931884c94993c1fc |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-20T13:38:41Z |
| publishDate | 2018-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-16fd143ca144419a931884c94993c1fc2022-12-21T19:38:52ZengPublic Library of Science (PLoS)PLoS ONE1932-62032018-01-011311e020556310.1371/journal.pone.0205563Can mesenchymal stem cells and their conditioned medium assist inflammatory chondrocytes recovery?Yu-Chun ChenYu-Wei ChangKinn Poay TanYi-Shan ShenYao-Horng WangChih-Hung ChangOsteoarthritis (OA), one of the most common joint disease, affects more than 80% of the population aged 70 or over. Mesenchymal stem cells (MSCs) show multi-potent differentiation and self-renewal capability, and, after exposure to an inflammatory environment, also exhibit immunosuppressive properties. In this study, we have used a model of lipopolysaccharide (LPS)-stimulated chondrocytes to evaluate MSC anti-inflammatory efficacy. The anti-inflammatory mechanism was tested in two cell-contained culture systems: (i) MSC-chondrocyte indirect contact system and (ii) MSC-chondrocyte direct contact system, and one cytokine-only culture system: MSC-conditioned medium (CM) system. Results showed that MSCs reduced chondrocyte inflammation through both paracrine secretion and cell-to-cell contact. The inflammation-associated, and free-radical-related genes were down-regulated significantly in the direct contact system on 24 h, however, the TNF-α. IL-6 were upregulated and aggrecan, COLII were downregulated on 72 h in direct contact system. Moreover, we found CM produced by MSC possess well therapeutic effect on inflammatory chondorcyte, and the 10-fold concentrated MSC-conditioned medium could down-regulated chondorcyte synthesis inflammation-associated, and free-radical-related genes, such as TNF-α, IL-1β, IL-6 and iNOS even treated for 72 h. In conclusion, MSC-CM showed great potential for MSC-based therapy for OA.http://europepmc.org/articles/PMC6248915?pdf=render |
| spellingShingle | Yu-Chun Chen Yu-Wei Chang Kinn Poay Tan Yi-Shan Shen Yao-Horng Wang Chih-Hung Chang Can mesenchymal stem cells and their conditioned medium assist inflammatory chondrocytes recovery? PLoS ONE |
| title | Can mesenchymal stem cells and their conditioned medium assist inflammatory chondrocytes recovery? |
| title_full | Can mesenchymal stem cells and their conditioned medium assist inflammatory chondrocytes recovery? |
| title_fullStr | Can mesenchymal stem cells and their conditioned medium assist inflammatory chondrocytes recovery? |
| title_full_unstemmed | Can mesenchymal stem cells and their conditioned medium assist inflammatory chondrocytes recovery? |
| title_short | Can mesenchymal stem cells and their conditioned medium assist inflammatory chondrocytes recovery? |
| title_sort | can mesenchymal stem cells and their conditioned medium assist inflammatory chondrocytes recovery |
| url | http://europepmc.org/articles/PMC6248915?pdf=render |
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