Immune Transcriptome and Secretome Differ between Human CD71<sup>+</sup> Erythroid Cells from Adult Bone Marrow and Fetal Liver Parenchyma
CD71<sup>+</sup> erythroid cells (CECs) were only known as erythrocyte progenitors not so long ago. In present times, however, they have been shown to be active players in immune regulation, especially in immunosuppression by the means of ROS, arginase-1 and arginase-2 production. Here,...
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2022-07-01
|
| Series: | Genes |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2073-4425/13/8/1333 |
| _version_ | 1797445180940353536 |
|---|---|
| author | Roman Perik-Zavodskii Olga Perik-Zavodskaya Yulia Shevchenko Vera Denisova Kirill Nazarov Irina Obleuhova Konstantin Zaitsev Sergey Sennikov |
| author_facet | Roman Perik-Zavodskii Olga Perik-Zavodskaya Yulia Shevchenko Vera Denisova Kirill Nazarov Irina Obleuhova Konstantin Zaitsev Sergey Sennikov |
| author_sort | Roman Perik-Zavodskii |
| collection | DOAJ |
| description | CD71<sup>+</sup> erythroid cells (CECs) were only known as erythrocyte progenitors not so long ago. In present times, however, they have been shown to be active players in immune regulation, especially in immunosuppression by the means of ROS, arginase-1 and arginase-2 production. Here, we uncover organ-of-origin differences in cytokine gene expression using NanoString and protein production using Bio-Plex between CECs from healthy human adult bone marrow and from human fetal liver parenchyma. Namely, healthy human adult bone marrow CECs both expressed and produced IFN-a, IL-1b, IL-8, IL-18 and MIF mRNA and protein, while human fetal liver parenchyma CECs expressed and produced IFN-a, IL15, IL18 and TNF-b mRNA and protein. We also detected TLR2 and TLR9 gene expression in both varieties of CECs and TLR1 and NOD2 gene expression in human fetal liver parenchyma CECs only. These observations suggest that there might be undiscovered roles in immune response for CECs. |
| first_indexed | 2024-03-09T13:23:00Z |
| format | Article |
| id | doaj.art-16fec0d22426458c9b0af4ca5765cdec |
| institution | Directory Open Access Journal |
| issn | 2073-4425 |
| language | English |
| last_indexed | 2024-03-09T13:23:00Z |
| publishDate | 2022-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Genes |
| spelling | doaj.art-16fec0d22426458c9b0af4ca5765cdec2023-11-30T21:27:19ZengMDPI AGGenes2073-44252022-07-01138133310.3390/genes13081333Immune Transcriptome and Secretome Differ between Human CD71<sup>+</sup> Erythroid Cells from Adult Bone Marrow and Fetal Liver ParenchymaRoman Perik-Zavodskii0Olga Perik-Zavodskaya1Yulia Shevchenko2Vera Denisova3Kirill Nazarov4Irina Obleuhova5Konstantin Zaitsev6Sergey Sennikov7Laboratory of Molecular Immunology, Federal State Budgetary Scientific Institution, Research Institute of Fundamental and Clinical Immunology, Yadrincevskaya 14, 630099 Novosibirsk, RussiaLaboratory of Molecular Immunology, Federal State Budgetary Scientific Institution, Research Institute of Fundamental and Clinical Immunology, Yadrincevskaya 14, 630099 Novosibirsk, RussiaLaboratory of Molecular Immunology, Federal State Budgetary Scientific Institution, Research Institute of Fundamental and Clinical Immunology, Yadrincevskaya 14, 630099 Novosibirsk, RussiaClinic of Immunopathology, Federal State Budgetary Scientific Institution, Research Institute of Fundamental and Clinical Immunology, Zalesskogo 2/1, 630047 Novosibirsk, RussiaLaboratory of Molecular Immunology, Federal State Budgetary Scientific Institution, Research Institute of Fundamental and Clinical Immunology, Yadrincevskaya 14, 630099 Novosibirsk, RussiaLaboratory of Molecular Immunology, Federal State Budgetary Scientific Institution, Research Institute of Fundamental and Clinical Immunology, Yadrincevskaya 14, 630099 Novosibirsk, RussiaFederal State Budgetary Scientific Institution “Siberian Federal Research and Clinical Center of the Federal Medicobiological Agency”, Rozy Lyuksemburg 5, 634009 Tomsk, RussiaLaboratory of Molecular Immunology, Federal State Budgetary Scientific Institution, Research Institute of Fundamental and Clinical Immunology, Yadrincevskaya 14, 630099 Novosibirsk, RussiaCD71<sup>+</sup> erythroid cells (CECs) were only known as erythrocyte progenitors not so long ago. In present times, however, they have been shown to be active players in immune regulation, especially in immunosuppression by the means of ROS, arginase-1 and arginase-2 production. Here, we uncover organ-of-origin differences in cytokine gene expression using NanoString and protein production using Bio-Plex between CECs from healthy human adult bone marrow and from human fetal liver parenchyma. Namely, healthy human adult bone marrow CECs both expressed and produced IFN-a, IL-1b, IL-8, IL-18 and MIF mRNA and protein, while human fetal liver parenchyma CECs expressed and produced IFN-a, IL15, IL18 and TNF-b mRNA and protein. We also detected TLR2 and TLR9 gene expression in both varieties of CECs and TLR1 and NOD2 gene expression in human fetal liver parenchyma CECs only. These observations suggest that there might be undiscovered roles in immune response for CECs.https://www.mdpi.com/2073-4425/13/8/1333CD71<sup>+</sup> erythroid cellsbone marrowfetal liver parenchymaMIFIL8TLR1 |
| spellingShingle | Roman Perik-Zavodskii Olga Perik-Zavodskaya Yulia Shevchenko Vera Denisova Kirill Nazarov Irina Obleuhova Konstantin Zaitsev Sergey Sennikov Immune Transcriptome and Secretome Differ between Human CD71<sup>+</sup> Erythroid Cells from Adult Bone Marrow and Fetal Liver Parenchyma Genes CD71<sup>+</sup> erythroid cells bone marrow fetal liver parenchyma MIF IL8 TLR1 |
| title | Immune Transcriptome and Secretome Differ between Human CD71<sup>+</sup> Erythroid Cells from Adult Bone Marrow and Fetal Liver Parenchyma |
| title_full | Immune Transcriptome and Secretome Differ between Human CD71<sup>+</sup> Erythroid Cells from Adult Bone Marrow and Fetal Liver Parenchyma |
| title_fullStr | Immune Transcriptome and Secretome Differ between Human CD71<sup>+</sup> Erythroid Cells from Adult Bone Marrow and Fetal Liver Parenchyma |
| title_full_unstemmed | Immune Transcriptome and Secretome Differ between Human CD71<sup>+</sup> Erythroid Cells from Adult Bone Marrow and Fetal Liver Parenchyma |
| title_short | Immune Transcriptome and Secretome Differ between Human CD71<sup>+</sup> Erythroid Cells from Adult Bone Marrow and Fetal Liver Parenchyma |
| title_sort | immune transcriptome and secretome differ between human cd71 sup sup erythroid cells from adult bone marrow and fetal liver parenchyma |
| topic | CD71<sup>+</sup> erythroid cells bone marrow fetal liver parenchyma MIF IL8 TLR1 |
| url | https://www.mdpi.com/2073-4425/13/8/1333 |
| work_keys_str_mv | AT romanperikzavodskii immunetranscriptomeandsecretomedifferbetweenhumancd71supsuperythroidcellsfromadultbonemarrowandfetalliverparenchyma AT olgaperikzavodskaya immunetranscriptomeandsecretomedifferbetweenhumancd71supsuperythroidcellsfromadultbonemarrowandfetalliverparenchyma AT yuliashevchenko immunetranscriptomeandsecretomedifferbetweenhumancd71supsuperythroidcellsfromadultbonemarrowandfetalliverparenchyma AT veradenisova immunetranscriptomeandsecretomedifferbetweenhumancd71supsuperythroidcellsfromadultbonemarrowandfetalliverparenchyma AT kirillnazarov immunetranscriptomeandsecretomedifferbetweenhumancd71supsuperythroidcellsfromadultbonemarrowandfetalliverparenchyma AT irinaobleuhova immunetranscriptomeandsecretomedifferbetweenhumancd71supsuperythroidcellsfromadultbonemarrowandfetalliverparenchyma AT konstantinzaitsev immunetranscriptomeandsecretomedifferbetweenhumancd71supsuperythroidcellsfromadultbonemarrowandfetalliverparenchyma AT sergeysennikov immunetranscriptomeandsecretomedifferbetweenhumancd71supsuperythroidcellsfromadultbonemarrowandfetalliverparenchyma |