Cerebral Organoids—Challenges to Establish a Brain Prototype
The new cellular models based on neural cells differentiated from induced pluripotent stem cells have greatly enhanced our understanding of human nervous system development. Highly efficient protocols for the differentiation of iPSCs into different types of neural cells have allowed the creation of...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-07-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/10/7/1790 |
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| author | Artem V. Eremeev Olga S. Lebedeva Margarita E. Bogomiakova Maria A. Lagarkova Alexandra N. Bogomazova |
| author_facet | Artem V. Eremeev Olga S. Lebedeva Margarita E. Bogomiakova Maria A. Lagarkova Alexandra N. Bogomazova |
| author_sort | Artem V. Eremeev |
| collection | DOAJ |
| description | The new cellular models based on neural cells differentiated from induced pluripotent stem cells have greatly enhanced our understanding of human nervous system development. Highly efficient protocols for the differentiation of iPSCs into different types of neural cells have allowed the creation of 2D models of many neurodegenerative diseases and nervous system development. However, the 2D culture of neurons is an imperfect model of the 3D brain tissue architecture represented by many functionally active cell types. The development of protocols for the differentiation of iPSCs into 3D cerebral organoids made it possible to establish a cellular model closest to native human brain tissue. Cerebral organoids are equally suitable for modeling various CNS pathologies, testing pharmacologically active substances, and utilization in regenerative medicine. Meanwhile, this technology is still at the initial stage of development. |
| first_indexed | 2024-03-10T09:42:49Z |
| format | Article |
| id | doaj.art-16ffbc40502a4eecab2b6496c242ae58 |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-10T09:42:49Z |
| publishDate | 2021-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-16ffbc40502a4eecab2b6496c242ae582023-11-22T03:30:08ZengMDPI AGCells2073-44092021-07-01107179010.3390/cells10071790Cerebral Organoids—Challenges to Establish a Brain PrototypeArtem V. Eremeev0Olga S. Lebedeva1Margarita E. Bogomiakova2Maria A. Lagarkova3Alexandra N. Bogomazova4Federal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, Malaya Pirogovskaya, 1a, 119435 Moscow, RussiaFederal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, Malaya Pirogovskaya, 1a, 119435 Moscow, RussiaFederal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, Malaya Pirogovskaya, 1a, 119435 Moscow, RussiaFederal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, Malaya Pirogovskaya, 1a, 119435 Moscow, RussiaFederal Research and Clinical Center of Physical-Chemical Medicine of Federal Medical Biological Agency, Malaya Pirogovskaya, 1a, 119435 Moscow, RussiaThe new cellular models based on neural cells differentiated from induced pluripotent stem cells have greatly enhanced our understanding of human nervous system development. Highly efficient protocols for the differentiation of iPSCs into different types of neural cells have allowed the creation of 2D models of many neurodegenerative diseases and nervous system development. However, the 2D culture of neurons is an imperfect model of the 3D brain tissue architecture represented by many functionally active cell types. The development of protocols for the differentiation of iPSCs into 3D cerebral organoids made it possible to establish a cellular model closest to native human brain tissue. Cerebral organoids are equally suitable for modeling various CNS pathologies, testing pharmacologically active substances, and utilization in regenerative medicine. Meanwhile, this technology is still at the initial stage of development.https://www.mdpi.com/2073-4409/10/7/1790iPSCsbrain organoidsdifferentiationelectrophysiologyneurotrophic factors |
| spellingShingle | Artem V. Eremeev Olga S. Lebedeva Margarita E. Bogomiakova Maria A. Lagarkova Alexandra N. Bogomazova Cerebral Organoids—Challenges to Establish a Brain Prototype Cells iPSCs brain organoids differentiation electrophysiology neurotrophic factors |
| title | Cerebral Organoids—Challenges to Establish a Brain Prototype |
| title_full | Cerebral Organoids—Challenges to Establish a Brain Prototype |
| title_fullStr | Cerebral Organoids—Challenges to Establish a Brain Prototype |
| title_full_unstemmed | Cerebral Organoids—Challenges to Establish a Brain Prototype |
| title_short | Cerebral Organoids—Challenges to Establish a Brain Prototype |
| title_sort | cerebral organoids challenges to establish a brain prototype |
| topic | iPSCs brain organoids differentiation electrophysiology neurotrophic factors |
| url | https://www.mdpi.com/2073-4409/10/7/1790 |
| work_keys_str_mv | AT artemveremeev cerebralorganoidschallengestoestablishabrainprototype AT olgaslebedeva cerebralorganoidschallengestoestablishabrainprototype AT margaritaebogomiakova cerebralorganoidschallengestoestablishabrainprototype AT mariaalagarkova cerebralorganoidschallengestoestablishabrainprototype AT alexandranbogomazova cerebralorganoidschallengestoestablishabrainprototype |