Integrative characterization of germ cell-specific genes from mouse spermatocyte UniGene library
<p>Abstract</p> <p>Background</p> <p>The primary regulator of spermatogenesis, a highly ordered and tightly regulated developmental process, is an intrinsic genetic program involving male germ cell-specific genes.</p> <p>Results</p> <p>We analyze...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2007-07-01
|
| Series: | BMC Genomics |
| Online Access: | http://www.biomedcentral.com/1471-2164/8/256 |
| _version_ | 1811265550079229952 |
|---|---|
| author | Eddy Edward M Cho Byung-Nam Kim Do Yi Chongil Han Cecil Park Inju Oh Jungsu Lee Jiae Choi Eunyoung Cho Chunghee |
| author_facet | Eddy Edward M Cho Byung-Nam Kim Do Yi Chongil Han Cecil Park Inju Oh Jungsu Lee Jiae Choi Eunyoung Cho Chunghee |
| author_sort | Eddy Edward M |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>The primary regulator of spermatogenesis, a highly ordered and tightly regulated developmental process, is an intrinsic genetic program involving male germ cell-specific genes.</p> <p>Results</p> <p>We analyzed the mouse spermatocyte UniGene library containing 2155 gene-oriented transcript clusters. We predict that 11% of these genes are testis-specific and systematically identified 24 authentic genes specifically and abundantly expressed in the testis via in <it>silico </it>and <it>in vitro </it>approaches. Northern blot analysis disclosed various transcript characteristics, such as expression level, size and the presence of isoform. Expression analysis revealed developmentally regulated and stage-specific expression patterns in all of the genes. We further analyzed the genes at the protein and cellular levels. Transfection assays performed using GC-2 cells provided information on the cellular characteristics of the gene products. In addition, antibodies were generated against proteins encoded by some of the genes to facilitate their identification and characterization in spermatogenic cells and sperm. Our data suggest that a number of the gene products are implicated in transcriptional regulation, nuclear integrity, sperm structure and motility, and fertilization. In particular, we found for the first time that Mm.333010, predicted to contain a trypsin-like serine protease domain, is a sperm acrosomal protein.</p> <p>Conclusion</p> <p>We identify 24 authentic genes with spermatogenic cell-specific expression, and provide comprehensive information about the genes. Our findings establish a new basis for future investigation into molecular mechanisms underlying male reproduction.</p> |
| first_indexed | 2024-04-12T20:24:42Z |
| format | Article |
| id | doaj.art-1704e06dcb1c41a7aee5316a325a336d |
| institution | Directory Open Access Journal |
| issn | 1471-2164 |
| language | English |
| last_indexed | 2024-04-12T20:24:42Z |
| publishDate | 2007-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj.art-1704e06dcb1c41a7aee5316a325a336d2022-12-22T03:17:53ZengBMCBMC Genomics1471-21642007-07-018125610.1186/1471-2164-8-256Integrative characterization of germ cell-specific genes from mouse spermatocyte UniGene libraryEddy Edward MCho Byung-NamKim DoYi ChongilHan CecilPark InjuOh JungsuLee JiaeChoi EunyoungCho Chunghee<p>Abstract</p> <p>Background</p> <p>The primary regulator of spermatogenesis, a highly ordered and tightly regulated developmental process, is an intrinsic genetic program involving male germ cell-specific genes.</p> <p>Results</p> <p>We analyzed the mouse spermatocyte UniGene library containing 2155 gene-oriented transcript clusters. We predict that 11% of these genes are testis-specific and systematically identified 24 authentic genes specifically and abundantly expressed in the testis via in <it>silico </it>and <it>in vitro </it>approaches. Northern blot analysis disclosed various transcript characteristics, such as expression level, size and the presence of isoform. Expression analysis revealed developmentally regulated and stage-specific expression patterns in all of the genes. We further analyzed the genes at the protein and cellular levels. Transfection assays performed using GC-2 cells provided information on the cellular characteristics of the gene products. In addition, antibodies were generated against proteins encoded by some of the genes to facilitate their identification and characterization in spermatogenic cells and sperm. Our data suggest that a number of the gene products are implicated in transcriptional regulation, nuclear integrity, sperm structure and motility, and fertilization. In particular, we found for the first time that Mm.333010, predicted to contain a trypsin-like serine protease domain, is a sperm acrosomal protein.</p> <p>Conclusion</p> <p>We identify 24 authentic genes with spermatogenic cell-specific expression, and provide comprehensive information about the genes. Our findings establish a new basis for future investigation into molecular mechanisms underlying male reproduction.</p>http://www.biomedcentral.com/1471-2164/8/256 |
| spellingShingle | Eddy Edward M Cho Byung-Nam Kim Do Yi Chongil Han Cecil Park Inju Oh Jungsu Lee Jiae Choi Eunyoung Cho Chunghee Integrative characterization of germ cell-specific genes from mouse spermatocyte UniGene library BMC Genomics |
| title | Integrative characterization of germ cell-specific genes from mouse spermatocyte UniGene library |
| title_full | Integrative characterization of germ cell-specific genes from mouse spermatocyte UniGene library |
| title_fullStr | Integrative characterization of germ cell-specific genes from mouse spermatocyte UniGene library |
| title_full_unstemmed | Integrative characterization of germ cell-specific genes from mouse spermatocyte UniGene library |
| title_short | Integrative characterization of germ cell-specific genes from mouse spermatocyte UniGene library |
| title_sort | integrative characterization of germ cell specific genes from mouse spermatocyte unigene library |
| url | http://www.biomedcentral.com/1471-2164/8/256 |
| work_keys_str_mv | AT eddyedwardm integrativecharacterizationofgermcellspecificgenesfrommousespermatocyteunigenelibrary AT chobyungnam integrativecharacterizationofgermcellspecificgenesfrommousespermatocyteunigenelibrary AT kimdo integrativecharacterizationofgermcellspecificgenesfrommousespermatocyteunigenelibrary AT yichongil integrativecharacterizationofgermcellspecificgenesfrommousespermatocyteunigenelibrary AT hancecil integrativecharacterizationofgermcellspecificgenesfrommousespermatocyteunigenelibrary AT parkinju integrativecharacterizationofgermcellspecificgenesfrommousespermatocyteunigenelibrary AT ohjungsu integrativecharacterizationofgermcellspecificgenesfrommousespermatocyteunigenelibrary AT leejiae integrativecharacterizationofgermcellspecificgenesfrommousespermatocyteunigenelibrary AT choieunyoung integrativecharacterizationofgermcellspecificgenesfrommousespermatocyteunigenelibrary AT chochunghee integrativecharacterizationofgermcellspecificgenesfrommousespermatocyteunigenelibrary |