Exogenous brassinosteroid alleviates calcium deficiency induced tip-burn by regulating calcium transport in Brassica rapa L. ssp. pekinensis
Mini Chinese cabbage (Brassica rapa L. ssp. Pekinensis) plays an important role in the supply of summer vegetables on the plateau in western China. In recent years, tip-burn has seriously affected the yield, quality and commodity value of mini Chinese cabbage. Calcium (Ca2+) deficiency is a key indu...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2023-02-01
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| Series: | Ecotoxicology and Environmental Safety |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0147651323000386 |
| _version_ | 1828046693698895872 |
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| author | Yutong Li Yue Wu Zhongqi Tang Xuemei Xiao Xueqin Gao Yali Qiao Jizhong Ma Linli Hu Jihua Yu |
| author_facet | Yutong Li Yue Wu Zhongqi Tang Xuemei Xiao Xueqin Gao Yali Qiao Jizhong Ma Linli Hu Jihua Yu |
| author_sort | Yutong Li |
| collection | DOAJ |
| description | Mini Chinese cabbage (Brassica rapa L. ssp. Pekinensis) plays an important role in the supply of summer vegetables on the plateau in western China. In recent years, tip-burn has seriously affected the yield, quality and commodity value of mini Chinese cabbage. Calcium (Ca2+) deficiency is a key inducer of tip-burn. As a new type plant hormone, brassinolide (BR) is involved in regulating a variety of biotic and abiotic stresses. To explore the alleviation role of BR in tip-burn caused by Ca2+ deficiency, a hydroponic experiment was conducted to study the relationship between BR and Ca2+ absorption and transport. The results showed that foliar spraying with 0.5 µM BR significantly reduced tip-burn incidence rate and disease index of mini Chinese cabbage caused by Ca2+ deficiency. Moreover, the dynamic monitoring results of tip-burn incidence rate showed that the value reached the highest on the ninth day after treatment. BR promoted the Ca2+ transport from roots to shoots and from outer leaves to inner leaves by increasing the activities of Ca2+-ATPase and H+-ATPase as well as the total ATP content, which provided power for Ca2+ transport. In addition, exogenous BR upregulated the relative expression levels of BrACA4, BrACA11, BrECA1, BrECA3, BrECA4, BrCAX1, BrCAS and BrCRT2, whereas Ca2+ deficiency induced down-regulation. In conclusion, exogenous BR can alleviate the Ca2+-deficiency induced tip-burn of mini Chinese cabbage by promoting the transport and distribution of Ca2+. |
| first_indexed | 2024-04-10T18:29:31Z |
| format | Article |
| id | doaj.art-1706542abfc14f978a25261979c169bc |
| institution | Directory Open Access Journal |
| issn | 0147-6513 |
| language | English |
| last_indexed | 2024-04-10T18:29:31Z |
| publishDate | 2023-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Ecotoxicology and Environmental Safety |
| spelling | doaj.art-1706542abfc14f978a25261979c169bc2023-02-02T04:47:00ZengElsevierEcotoxicology and Environmental Safety0147-65132023-02-01251114534Exogenous brassinosteroid alleviates calcium deficiency induced tip-burn by regulating calcium transport in Brassica rapa L. ssp. pekinensisYutong Li0Yue Wu1Zhongqi Tang2Xuemei Xiao3Xueqin Gao4Yali Qiao5Jizhong Ma6Linli Hu7Jihua Yu8College of Horticulture, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Horticulture, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Horticulture, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Horticulture, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Horticulture, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Horticulture, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Horticulture, Gansu Agricultural University, Lanzhou 730070, ChinaCollege of Horticulture, Gansu Agricultural University, Lanzhou 730070, China; State Key Laboratory of Arid-land Crop Science, Gansu Agricultural University, Lanzhou 730070, China; Corresponding authors at: College of Horticulture, Gansu Agricultural University, Lanzhou 730070, China.College of Horticulture, Gansu Agricultural University, Lanzhou 730070, China; State Key Laboratory of Arid-land Crop Science, Gansu Agricultural University, Lanzhou 730070, China; Corresponding authors at: College of Horticulture, Gansu Agricultural University, Lanzhou 730070, China.Mini Chinese cabbage (Brassica rapa L. ssp. Pekinensis) plays an important role in the supply of summer vegetables on the plateau in western China. In recent years, tip-burn has seriously affected the yield, quality and commodity value of mini Chinese cabbage. Calcium (Ca2+) deficiency is a key inducer of tip-burn. As a new type plant hormone, brassinolide (BR) is involved in regulating a variety of biotic and abiotic stresses. To explore the alleviation role of BR in tip-burn caused by Ca2+ deficiency, a hydroponic experiment was conducted to study the relationship between BR and Ca2+ absorption and transport. The results showed that foliar spraying with 0.5 µM BR significantly reduced tip-burn incidence rate and disease index of mini Chinese cabbage caused by Ca2+ deficiency. Moreover, the dynamic monitoring results of tip-burn incidence rate showed that the value reached the highest on the ninth day after treatment. BR promoted the Ca2+ transport from roots to shoots and from outer leaves to inner leaves by increasing the activities of Ca2+-ATPase and H+-ATPase as well as the total ATP content, which provided power for Ca2+ transport. In addition, exogenous BR upregulated the relative expression levels of BrACA4, BrACA11, BrECA1, BrECA3, BrECA4, BrCAX1, BrCAS and BrCRT2, whereas Ca2+ deficiency induced down-regulation. In conclusion, exogenous BR can alleviate the Ca2+-deficiency induced tip-burn of mini Chinese cabbage by promoting the transport and distribution of Ca2+.http://www.sciencedirect.com/science/article/pii/S0147651323000386Tip-burnBrassinolideCalcium deficiencyCalcium distributionMini Chinese cabbage |
| spellingShingle | Yutong Li Yue Wu Zhongqi Tang Xuemei Xiao Xueqin Gao Yali Qiao Jizhong Ma Linli Hu Jihua Yu Exogenous brassinosteroid alleviates calcium deficiency induced tip-burn by regulating calcium transport in Brassica rapa L. ssp. pekinensis Ecotoxicology and Environmental Safety Tip-burn Brassinolide Calcium deficiency Calcium distribution Mini Chinese cabbage |
| title | Exogenous brassinosteroid alleviates calcium deficiency induced tip-burn by regulating calcium transport in Brassica rapa L. ssp. pekinensis |
| title_full | Exogenous brassinosteroid alleviates calcium deficiency induced tip-burn by regulating calcium transport in Brassica rapa L. ssp. pekinensis |
| title_fullStr | Exogenous brassinosteroid alleviates calcium deficiency induced tip-burn by regulating calcium transport in Brassica rapa L. ssp. pekinensis |
| title_full_unstemmed | Exogenous brassinosteroid alleviates calcium deficiency induced tip-burn by regulating calcium transport in Brassica rapa L. ssp. pekinensis |
| title_short | Exogenous brassinosteroid alleviates calcium deficiency induced tip-burn by regulating calcium transport in Brassica rapa L. ssp. pekinensis |
| title_sort | exogenous brassinosteroid alleviates calcium deficiency induced tip burn by regulating calcium transport in brassica rapa l ssp pekinensis |
| topic | Tip-burn Brassinolide Calcium deficiency Calcium distribution Mini Chinese cabbage |
| url | http://www.sciencedirect.com/science/article/pii/S0147651323000386 |
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