Effect of Ishophloroglucin A Isolated from <i>Ishige okamurae</i> on In Vitro Osteoclastogenesis and Osteoblastogenesis
The balance between bone-resorbing osteoclasts and bone-forming osteoblasts is essential for the bone remodeling process. This study aimed to investigate the effect of Ishophloroglucin A (IPA) isolated from <i>Ishige okamurae</i> on the function of osteoclasts and osteoblasts in vitro. F...
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MDPI AG
2023-06-01
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| Series: | Marine Drugs |
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| Online Access: | https://www.mdpi.com/1660-3397/21/7/377 |
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| author | Su-Hyeon Cho Hyun-Soo Kim Hye-Yeon Jung Jae-Il Park You-Jee Jang Juhee Ahn Kil-Nam Kim |
| author_facet | Su-Hyeon Cho Hyun-Soo Kim Hye-Yeon Jung Jae-Il Park You-Jee Jang Juhee Ahn Kil-Nam Kim |
| author_sort | Su-Hyeon Cho |
| collection | DOAJ |
| description | The balance between bone-resorbing osteoclasts and bone-forming osteoblasts is essential for the bone remodeling process. This study aimed to investigate the effect of Ishophloroglucin A (IPA) isolated from <i>Ishige okamurae</i> on the function of osteoclasts and osteoblasts in vitro. First, we demonstrated the effect of IPA on osteoclastogenesis in receptor activator of nuclear factor κB ligand (RANKL)-induced RAW 264.7 cells. IPA inhibited the tartrate-resistant acid phosphatase (TRAP) activity and osteoclast differentiation in RANKL-induced RAW 264.7 cells. Moreover, it inhibited the RANKL-induced osteoclast-related factors, such as TRAP, matrix metalloproteinase-9 (MMP-9), and calcitonin receptor (CTR), and transcription factors, such as nuclear factor of activated T cells 1 (NFATc1) and c-Fos. IPA significantly suppressed RANKL-activated extracellular signal-regulated kinase (ERK), and NF-κB in RAW 264.7 cells. Our data indicated that the ERK and NF-κB pathways were associated with the osteoclastogenesis inhibitory activity of IPA. Next, we demonstrated the effect of IPA on osteoblastogenesis in MG-63 cells. IPA significantly promoted alkaline phosphatase (ALP) activity in MG-63 cells, along with the osteoblast differentiation-related markers bone morphogenetic protein 2 (BMP2), type 1 collage (COL1), p-Smad1/5/8, and Runx2, by activating the MAPK signaling pathways. Taken together, the study indicated that IPA could be effective in treating bone diseases, such as osteoporosis. |
| first_indexed | 2024-03-11T00:53:30Z |
| format | Article |
| id | doaj.art-17086f6658dc4cc8b5a4b8d7d18790cd |
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| language | English |
| last_indexed | 2024-03-11T00:53:30Z |
| publishDate | 2023-06-01 |
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| series | Marine Drugs |
| spelling | doaj.art-17086f6658dc4cc8b5a4b8d7d18790cd2023-11-18T20:13:56ZengMDPI AGMarine Drugs1660-33972023-06-0121737710.3390/md21070377Effect of Ishophloroglucin A Isolated from <i>Ishige okamurae</i> on In Vitro Osteoclastogenesis and OsteoblastogenesisSu-Hyeon Cho0Hyun-Soo Kim1Hye-Yeon Jung2Jae-Il Park3You-Jee Jang4Juhee Ahn5Kil-Nam Kim6Chuncheon Center, Korea Basic Science Institute (KBSI), Chuncheon 24341, Republic of KoreaNational Marine Biodiversity Institute of Korea, Seocheon 33662, Republic of KoreaGwangju Center, Korea Basic Science Institute (KBSI), Gwangju 61751, Republic of KoreaGwangju Center, Korea Basic Science Institute (KBSI), Gwangju 61751, Republic of KoreaDepartment of Biomedical Laboratory Science, Honam University, Gwangju 62399, Republic of KoreaDepartment of Medical Biomaterials Engineering, College of Biomedical Sciences, Kangwon National University, Chuncheon 24341, Republic of KoreaChuncheon Center, Korea Basic Science Institute (KBSI), Chuncheon 24341, Republic of KoreaThe balance between bone-resorbing osteoclasts and bone-forming osteoblasts is essential for the bone remodeling process. This study aimed to investigate the effect of Ishophloroglucin A (IPA) isolated from <i>Ishige okamurae</i> on the function of osteoclasts and osteoblasts in vitro. First, we demonstrated the effect of IPA on osteoclastogenesis in receptor activator of nuclear factor κB ligand (RANKL)-induced RAW 264.7 cells. IPA inhibited the tartrate-resistant acid phosphatase (TRAP) activity and osteoclast differentiation in RANKL-induced RAW 264.7 cells. Moreover, it inhibited the RANKL-induced osteoclast-related factors, such as TRAP, matrix metalloproteinase-9 (MMP-9), and calcitonin receptor (CTR), and transcription factors, such as nuclear factor of activated T cells 1 (NFATc1) and c-Fos. IPA significantly suppressed RANKL-activated extracellular signal-regulated kinase (ERK), and NF-κB in RAW 264.7 cells. Our data indicated that the ERK and NF-κB pathways were associated with the osteoclastogenesis inhibitory activity of IPA. Next, we demonstrated the effect of IPA on osteoblastogenesis in MG-63 cells. IPA significantly promoted alkaline phosphatase (ALP) activity in MG-63 cells, along with the osteoblast differentiation-related markers bone morphogenetic protein 2 (BMP2), type 1 collage (COL1), p-Smad1/5/8, and Runx2, by activating the MAPK signaling pathways. Taken together, the study indicated that IPA could be effective in treating bone diseases, such as osteoporosis.https://www.mdpi.com/1660-3397/21/7/377Ishophlorogulcin A<i>Ishige okamurae</i>osteoclastogenesisosteoblastogenesisRAW 264.7 cellsMG-63 cells |
| spellingShingle | Su-Hyeon Cho Hyun-Soo Kim Hye-Yeon Jung Jae-Il Park You-Jee Jang Juhee Ahn Kil-Nam Kim Effect of Ishophloroglucin A Isolated from <i>Ishige okamurae</i> on In Vitro Osteoclastogenesis and Osteoblastogenesis Marine Drugs Ishophlorogulcin A <i>Ishige okamurae</i> osteoclastogenesis osteoblastogenesis RAW 264.7 cells MG-63 cells |
| title | Effect of Ishophloroglucin A Isolated from <i>Ishige okamurae</i> on In Vitro Osteoclastogenesis and Osteoblastogenesis |
| title_full | Effect of Ishophloroglucin A Isolated from <i>Ishige okamurae</i> on In Vitro Osteoclastogenesis and Osteoblastogenesis |
| title_fullStr | Effect of Ishophloroglucin A Isolated from <i>Ishige okamurae</i> on In Vitro Osteoclastogenesis and Osteoblastogenesis |
| title_full_unstemmed | Effect of Ishophloroglucin A Isolated from <i>Ishige okamurae</i> on In Vitro Osteoclastogenesis and Osteoblastogenesis |
| title_short | Effect of Ishophloroglucin A Isolated from <i>Ishige okamurae</i> on In Vitro Osteoclastogenesis and Osteoblastogenesis |
| title_sort | effect of ishophloroglucin a isolated from i ishige okamurae i on in vitro osteoclastogenesis and osteoblastogenesis |
| topic | Ishophlorogulcin A <i>Ishige okamurae</i> osteoclastogenesis osteoblastogenesis RAW 264.7 cells MG-63 cells |
| url | https://www.mdpi.com/1660-3397/21/7/377 |
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