Revealing the associated microflora hosted by the globally significant parasite Trichostrongylus colubriformis
Abstract Trichostrongylus colubriformis is a parasitic helminth that primarily infects small ruminants, causing substantial economic losses in the livestock industry. Exploring the microbiome of this helminth might provide insights into the potential influence of its microbial community on the paras...
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| Format: | Article |
| Language: | English |
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Nature Portfolio
2024-02-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-024-53772-z |
| _version_ | 1797274630566707200 |
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| author | Erwin A. Paz Eng Guan Chua Dieter G. Palmer Johan C. Greeff Shimin Liu Carolina Cheuquemán Shamshad Ul Hassan Graeme B. Martin Chin Yen Tay |
| author_facet | Erwin A. Paz Eng Guan Chua Dieter G. Palmer Johan C. Greeff Shimin Liu Carolina Cheuquemán Shamshad Ul Hassan Graeme B. Martin Chin Yen Tay |
| author_sort | Erwin A. Paz |
| collection | DOAJ |
| description | Abstract Trichostrongylus colubriformis is a parasitic helminth that primarily infects small ruminants, causing substantial economic losses in the livestock industry. Exploring the microbiome of this helminth might provide insights into the potential influence of its microbial community on the parasite’s survival. We characterised the intestinal microbiome of T. colubriformis that had been collected from the duodenum of sheep, and compared the helminth microbiome with the duodenal microbiome of its host, aiming to identify contributions from the helminth’s environment. At the same time, we explored the isolation of fastidious organisms from the harvested helminth. Primary alpha and beta diversity analyses of bacterial species revealed statistically significant differences between the parasite and the host, in terms of species richness and ecological composition. 16S rRNA differential abundance analysis showed that Mycoplasmoides and Stenotrophomonas were significantly present in T. colubriformis but not in the duodenal microbiome of the sheep. Furthermore, two bacteria, Aeromonas caviae and Aeromonas hydrophila, were isolated from T. colubriformis. Examinations of the genome highlight differences in genome size and profiles of antimicrobial resistance genes. Our results suggest that T. colubriformis carries a specific bacterial community that could be supporting the helminth’s long-term survival in the host’s digestive system. |
| first_indexed | 2024-03-07T15:01:03Z |
| format | Article |
| id | doaj.art-170bf4a52f5a4128be69fe6dd0b5a76c |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-03-07T15:01:03Z |
| publishDate | 2024-02-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-170bf4a52f5a4128be69fe6dd0b5a76c2024-03-05T19:07:46ZengNature PortfolioScientific Reports2045-23222024-02-0114111010.1038/s41598-024-53772-zRevealing the associated microflora hosted by the globally significant parasite Trichostrongylus colubriformisErwin A. Paz0Eng Guan Chua1Dieter G. Palmer2Johan C. Greeff3Shimin Liu4Carolina Cheuquemán5Shamshad Ul Hassan6Graeme B. Martin7Chin Yen Tay8UWA Institute of Agriculture, The University of Western AustraliaHelicobacter Research Laboratory, The Marshall Centre for Infectious Disease Research and Training, School of Biomedical Sciences, The University of Western AustraliaDepartment of Primary Industries and Regional Development Western AustraliaUWA Institute of Agriculture, The University of Western AustraliaUWA Institute of Agriculture, The University of Western AustraliaMedicina Veterinaria, Facultad de Ciencias Agropecuarias, Universidad del AlbaUWA Institute of Agriculture, The University of Western AustraliaUWA Institute of Agriculture, The University of Western AustraliaHelicobacter Research Laboratory, The Marshall Centre for Infectious Disease Research and Training, School of Biomedical Sciences, The University of Western AustraliaAbstract Trichostrongylus colubriformis is a parasitic helminth that primarily infects small ruminants, causing substantial economic losses in the livestock industry. Exploring the microbiome of this helminth might provide insights into the potential influence of its microbial community on the parasite’s survival. We characterised the intestinal microbiome of T. colubriformis that had been collected from the duodenum of sheep, and compared the helminth microbiome with the duodenal microbiome of its host, aiming to identify contributions from the helminth’s environment. At the same time, we explored the isolation of fastidious organisms from the harvested helminth. Primary alpha and beta diversity analyses of bacterial species revealed statistically significant differences between the parasite and the host, in terms of species richness and ecological composition. 16S rRNA differential abundance analysis showed that Mycoplasmoides and Stenotrophomonas were significantly present in T. colubriformis but not in the duodenal microbiome of the sheep. Furthermore, two bacteria, Aeromonas caviae and Aeromonas hydrophila, were isolated from T. colubriformis. Examinations of the genome highlight differences in genome size and profiles of antimicrobial resistance genes. Our results suggest that T. colubriformis carries a specific bacterial community that could be supporting the helminth’s long-term survival in the host’s digestive system.https://doi.org/10.1038/s41598-024-53772-z |
| spellingShingle | Erwin A. Paz Eng Guan Chua Dieter G. Palmer Johan C. Greeff Shimin Liu Carolina Cheuquemán Shamshad Ul Hassan Graeme B. Martin Chin Yen Tay Revealing the associated microflora hosted by the globally significant parasite Trichostrongylus colubriformis Scientific Reports |
| title | Revealing the associated microflora hosted by the globally significant parasite Trichostrongylus colubriformis |
| title_full | Revealing the associated microflora hosted by the globally significant parasite Trichostrongylus colubriformis |
| title_fullStr | Revealing the associated microflora hosted by the globally significant parasite Trichostrongylus colubriformis |
| title_full_unstemmed | Revealing the associated microflora hosted by the globally significant parasite Trichostrongylus colubriformis |
| title_short | Revealing the associated microflora hosted by the globally significant parasite Trichostrongylus colubriformis |
| title_sort | revealing the associated microflora hosted by the globally significant parasite trichostrongylus colubriformis |
| url | https://doi.org/10.1038/s41598-024-53772-z |
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