Rat liver bile acid CoA:amino acid N-acyltransferase
Bile acid CoA:amino acid N-acyltransferase (BAT) is responsible for the amidation of bile acids with the amino acids taurine and glycine. Rat liver BAT (rBAT) cDNA was isolated from a rat liver λZAP cDNA library and expressed in Sf9 insect cells using a baculoviral vector. rBAT displayed 65% amino a...
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2003-12-01
|
| Series: | Journal of Lipid Research |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S0022227520319416 |
| _version_ | 1818646767970811904 |
|---|---|
| author | Dongning He Stephen Barnes Charles N. Falany |
| author_facet | Dongning He Stephen Barnes Charles N. Falany |
| author_sort | Dongning He |
| collection | DOAJ |
| description | Bile acid CoA:amino acid N-acyltransferase (BAT) is responsible for the amidation of bile acids with the amino acids taurine and glycine. Rat liver BAT (rBAT) cDNA was isolated from a rat liver λZAP cDNA library and expressed in Sf9 insect cells using a baculoviral vector. rBAT displayed 65% amino acid sequence homology with human BAT (hBAT) and 85% homology with mouse BAT (mBAT). Similar to hBAT, expressed rBAT was capable of forming both taurine and glycine conjugates with cholyl-CoA. mBAT, which is highly homologous to rBAT, forms only taurine conjugated bile acids (Falany, C. N., H. Fortinberry, E. H. Leiter, and S. Barnes. 1997. Cloning and expression of mouse liver bile acid CoA: Amino acid N-acyltransferase. J. Lipid Res. 38: 86–95). Immunoblot analysis of rat tissues detected rBAT only in rat liver cytosol following homogenization and ultracentrifugation. Subcellular localization of rBAT detected activity and immunoreactive protein in both cytosol and isolated peroxisomes. Rat bile acid CoA ligase (rBAL), the enzyme responsible for the formation of bile acid CoA esters, was detected only in rat liver microsomes. Treatment of rats with clofibrate, a known peroxisomal proliferator, significantly induced rBAT activity, message, and immunoreactive protein in rat liver. Peroxisomal membrane protein-70, a marker for peroxisomes, was also induced by clofibrate, whereas rBAL activity and protein amount were not affected.In summary, rBAT is capable of forming both taurine and glycine bile acid conjugates and the enzyme is localized primarily in peroxisomes in rat liver. |
| first_indexed | 2024-12-17T00:51:53Z |
| format | Article |
| id | doaj.art-170eaadbe5134e699f282c623c2b24b7 |
| institution | Directory Open Access Journal |
| issn | 0022-2275 |
| language | English |
| last_indexed | 2024-12-17T00:51:53Z |
| publishDate | 2003-12-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Lipid Research |
| spelling | doaj.art-170eaadbe5134e699f282c623c2b24b72022-12-21T22:09:44ZengElsevierJournal of Lipid Research0022-22752003-12-01441222422249Rat liver bile acid CoA:amino acid N-acyltransferaseDongning He0Stephen Barnes1Charles N. Falany2Department of Pharmacology and Toxicology, University of Alabama at Birmingham, Birmingham, AL 35294Department of Pharmacology and Toxicology, University of Alabama at Birmingham, Birmingham, AL 35294Department of Pharmacology and Toxicology, University of Alabama at Birmingham, Birmingham, AL 35294Bile acid CoA:amino acid N-acyltransferase (BAT) is responsible for the amidation of bile acids with the amino acids taurine and glycine. Rat liver BAT (rBAT) cDNA was isolated from a rat liver λZAP cDNA library and expressed in Sf9 insect cells using a baculoviral vector. rBAT displayed 65% amino acid sequence homology with human BAT (hBAT) and 85% homology with mouse BAT (mBAT). Similar to hBAT, expressed rBAT was capable of forming both taurine and glycine conjugates with cholyl-CoA. mBAT, which is highly homologous to rBAT, forms only taurine conjugated bile acids (Falany, C. N., H. Fortinberry, E. H. Leiter, and S. Barnes. 1997. Cloning and expression of mouse liver bile acid CoA: Amino acid N-acyltransferase. J. Lipid Res. 38: 86–95). Immunoblot analysis of rat tissues detected rBAT only in rat liver cytosol following homogenization and ultracentrifugation. Subcellular localization of rBAT detected activity and immunoreactive protein in both cytosol and isolated peroxisomes. Rat bile acid CoA ligase (rBAL), the enzyme responsible for the formation of bile acid CoA esters, was detected only in rat liver microsomes. Treatment of rats with clofibrate, a known peroxisomal proliferator, significantly induced rBAT activity, message, and immunoreactive protein in rat liver. Peroxisomal membrane protein-70, a marker for peroxisomes, was also induced by clofibrate, whereas rBAL activity and protein amount were not affected.In summary, rBAT is capable of forming both taurine and glycine bile acid conjugates and the enzyme is localized primarily in peroxisomes in rat liver.http://www.sciencedirect.com/science/article/pii/S0022227520319416inductionbile acid ligaseperoxisomal membrane protein-70 |
| spellingShingle | Dongning He Stephen Barnes Charles N. Falany Rat liver bile acid CoA:amino acid N-acyltransferase Journal of Lipid Research induction bile acid ligase peroxisomal membrane protein-70 |
| title | Rat liver bile acid CoA:amino acid N-acyltransferase |
| title_full | Rat liver bile acid CoA:amino acid N-acyltransferase |
| title_fullStr | Rat liver bile acid CoA:amino acid N-acyltransferase |
| title_full_unstemmed | Rat liver bile acid CoA:amino acid N-acyltransferase |
| title_short | Rat liver bile acid CoA:amino acid N-acyltransferase |
| title_sort | rat liver bile acid coa amino acid n acyltransferase |
| topic | induction bile acid ligase peroxisomal membrane protein-70 |
| url | http://www.sciencedirect.com/science/article/pii/S0022227520319416 |
| work_keys_str_mv | AT dongninghe ratliverbileacidcoaaminoacidnacyltransferase AT stephenbarnes ratliverbileacidcoaaminoacidnacyltransferase AT charlesnfalany ratliverbileacidcoaaminoacidnacyltransferase |