A yeast platform for high-level synthesis of tetrahydroisoquinoline alkaloids
Plants synthesize more than 3000 tetrahydroisoquinoline (THIQ) alkaloids, but only a few of them have been produced by engineered microbes and titers are very low. Here, the authors increase (S)-reticuline titer to 4.6 g/L and repurpose the yeast Ehrlich pathway to synthesize a diverse array of THIQ...
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Format: | Article |
Language: | English |
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Nature Portfolio
2020-07-01
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Series: | Nature Communications |
Online Access: | https://doi.org/10.1038/s41467-020-17172-x |
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author | Michael E. Pyne Kaspar Kevvai Parbir S. Grewal Lauren Narcross Brian Choi Leanne Bourgeois John E. Dueber Vincent J. J. Martin |
author_facet | Michael E. Pyne Kaspar Kevvai Parbir S. Grewal Lauren Narcross Brian Choi Leanne Bourgeois John E. Dueber Vincent J. J. Martin |
author_sort | Michael E. Pyne |
collection | DOAJ |
description | Plants synthesize more than 3000 tetrahydroisoquinoline (THIQ) alkaloids, but only a few of them have been produced by engineered microbes and titers are very low. Here, the authors increase (S)-reticuline titer to 4.6 g/L and repurpose the yeast Ehrlich pathway to synthesize a diverse array of THIQ scaffolds. |
first_indexed | 2024-12-18T04:22:11Z |
format | Article |
id | doaj.art-1711736c3c694987b81eeec5d825890d |
institution | Directory Open Access Journal |
issn | 2041-1723 |
language | English |
last_indexed | 2024-12-18T04:22:11Z |
publishDate | 2020-07-01 |
publisher | Nature Portfolio |
record_format | Article |
series | Nature Communications |
spelling | doaj.art-1711736c3c694987b81eeec5d825890d2022-12-21T21:21:12ZengNature PortfolioNature Communications2041-17232020-07-0111111010.1038/s41467-020-17172-xA yeast platform for high-level synthesis of tetrahydroisoquinoline alkaloidsMichael E. Pyne0Kaspar Kevvai1Parbir S. Grewal2Lauren Narcross3Brian Choi4Leanne Bourgeois5John E. Dueber6Vincent J. J. Martin7Department of Biology, Concordia UniversityDepartment of Biology, Concordia UniversityDepartment of Chemical and Biomolecular Engineering, University of California, BerkeleyDepartment of Biology, Concordia UniversityDepartment of Chemical and Biomolecular Engineering, University of California, BerkeleyDepartment of Biology, Concordia UniversityDepartment of Bioengineering, University of California, BerkeleyDepartment of Biology, Concordia UniversityPlants synthesize more than 3000 tetrahydroisoquinoline (THIQ) alkaloids, but only a few of them have been produced by engineered microbes and titers are very low. Here, the authors increase (S)-reticuline titer to 4.6 g/L and repurpose the yeast Ehrlich pathway to synthesize a diverse array of THIQ scaffolds.https://doi.org/10.1038/s41467-020-17172-x |
spellingShingle | Michael E. Pyne Kaspar Kevvai Parbir S. Grewal Lauren Narcross Brian Choi Leanne Bourgeois John E. Dueber Vincent J. J. Martin A yeast platform for high-level synthesis of tetrahydroisoquinoline alkaloids Nature Communications |
title | A yeast platform for high-level synthesis of tetrahydroisoquinoline alkaloids |
title_full | A yeast platform for high-level synthesis of tetrahydroisoquinoline alkaloids |
title_fullStr | A yeast platform for high-level synthesis of tetrahydroisoquinoline alkaloids |
title_full_unstemmed | A yeast platform for high-level synthesis of tetrahydroisoquinoline alkaloids |
title_short | A yeast platform for high-level synthesis of tetrahydroisoquinoline alkaloids |
title_sort | yeast platform for high level synthesis of tetrahydroisoquinoline alkaloids |
url | https://doi.org/10.1038/s41467-020-17172-x |
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