A yeast platform for high-level synthesis of tetrahydroisoquinoline alkaloids

Plants synthesize more than 3000 tetrahydroisoquinoline (THIQ) alkaloids, but only a few of them have been produced by engineered microbes and titers are very low. Here, the authors increase (S)-reticuline titer to 4.6 g/L and repurpose the yeast Ehrlich pathway to synthesize a diverse array of THIQ...

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Main Authors: Michael E. Pyne, Kaspar Kevvai, Parbir S. Grewal, Lauren Narcross, Brian Choi, Leanne Bourgeois, John E. Dueber, Vincent J. J. Martin
Format: Article
Language:English
Published: Nature Portfolio 2020-07-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-020-17172-x
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author Michael E. Pyne
Kaspar Kevvai
Parbir S. Grewal
Lauren Narcross
Brian Choi
Leanne Bourgeois
John E. Dueber
Vincent J. J. Martin
author_facet Michael E. Pyne
Kaspar Kevvai
Parbir S. Grewal
Lauren Narcross
Brian Choi
Leanne Bourgeois
John E. Dueber
Vincent J. J. Martin
author_sort Michael E. Pyne
collection DOAJ
description Plants synthesize more than 3000 tetrahydroisoquinoline (THIQ) alkaloids, but only a few of them have been produced by engineered microbes and titers are very low. Here, the authors increase (S)-reticuline titer to 4.6 g/L and repurpose the yeast Ehrlich pathway to synthesize a diverse array of THIQ scaffolds.
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spelling doaj.art-1711736c3c694987b81eeec5d825890d2022-12-21T21:21:12ZengNature PortfolioNature Communications2041-17232020-07-0111111010.1038/s41467-020-17172-xA yeast platform for high-level synthesis of tetrahydroisoquinoline alkaloidsMichael E. Pyne0Kaspar Kevvai1Parbir S. Grewal2Lauren Narcross3Brian Choi4Leanne Bourgeois5John E. Dueber6Vincent J. J. Martin7Department of Biology, Concordia UniversityDepartment of Biology, Concordia UniversityDepartment of Chemical and Biomolecular Engineering, University of California, BerkeleyDepartment of Biology, Concordia UniversityDepartment of Chemical and Biomolecular Engineering, University of California, BerkeleyDepartment of Biology, Concordia UniversityDepartment of Bioengineering, University of California, BerkeleyDepartment of Biology, Concordia UniversityPlants synthesize more than 3000 tetrahydroisoquinoline (THIQ) alkaloids, but only a few of them have been produced by engineered microbes and titers are very low. Here, the authors increase (S)-reticuline titer to 4.6 g/L and repurpose the yeast Ehrlich pathway to synthesize a diverse array of THIQ scaffolds.https://doi.org/10.1038/s41467-020-17172-x
spellingShingle Michael E. Pyne
Kaspar Kevvai
Parbir S. Grewal
Lauren Narcross
Brian Choi
Leanne Bourgeois
John E. Dueber
Vincent J. J. Martin
A yeast platform for high-level synthesis of tetrahydroisoquinoline alkaloids
Nature Communications
title A yeast platform for high-level synthesis of tetrahydroisoquinoline alkaloids
title_full A yeast platform for high-level synthesis of tetrahydroisoquinoline alkaloids
title_fullStr A yeast platform for high-level synthesis of tetrahydroisoquinoline alkaloids
title_full_unstemmed A yeast platform for high-level synthesis of tetrahydroisoquinoline alkaloids
title_short A yeast platform for high-level synthesis of tetrahydroisoquinoline alkaloids
title_sort yeast platform for high level synthesis of tetrahydroisoquinoline alkaloids
url https://doi.org/10.1038/s41467-020-17172-x
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