Inhibition of cell growth by cellular differentiation into adipocyte-like cells in dexamethasone sensitive cancer cell lines
The stress responses in human body lead to secretion of cortisol hormone. The present study investigated the cellular responses on cell growth and cellular differentiation into adipocytes by exposure of synthetic stress hormone, dexamethasone (DEX) in various human cancer and normal cells. After pro...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2018-05-01
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| Series: | Animal Cells and Systems |
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| Online Access: | http://dx.doi.org/10.1080/19768354.2018.1476408 |
| _version_ | 1818244092398665728 |
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| author | Hea-In Kim Sun-Ha Moon Won-Cheol Lee Hyeon-Jeong Lee Sharath Belame Shivakumar Sung-Ho Lee Bong-Wook Park Gyu-Jin Rho Byeong-Gyun Jeon |
| author_facet | Hea-In Kim Sun-Ha Moon Won-Cheol Lee Hyeon-Jeong Lee Sharath Belame Shivakumar Sung-Ho Lee Bong-Wook Park Gyu-Jin Rho Byeong-Gyun Jeon |
| author_sort | Hea-In Kim |
| collection | DOAJ |
| description | The stress responses in human body lead to secretion of cortisol hormone. The present study investigated the cellular responses on cell growth and cellular differentiation into adipocytes by exposure of synthetic stress hormone, dexamethasone (DEX) in various human cancer and normal cells. After prolonged exposure of cells with 1 μg/ml DEX for 2 weeks, population doubling time (PDT) was significantly (P < .05) increased by inhibited cell growth in A-549 and MCF-7 cancer cells, and was unchanged in MDA-MB-231 cancer cells, normal MRC-5 fibroblasts, umbilical cord matrix-derived mesenchymal stem cells (UCMSCs) and dental papilla tissue-derived mesenchymal stem cells (DSCs). Whereas, PDT was significantly (P < .05) decreased in U87-MG cancer cells by increased cell growth. Glucose uptake was significantly (P < .05) increased in all the cancer cell lines compared to that in normal cell lines. Further, adiposome-like vesicles were noted in A-549 and MCF-7 cancer cells indicating retarded cell growth by DEX treatment, and the vesicles were stained with Oil-Red O solution. Further, the expression of adipocyte-specific genes such as glucose transporter type 4 (GLUT4), glucocorticoid receptors β (GRβ) and peroxisome proliferator-activated receptor γ (PPARγ) were significantly (P < .05) increased in A-549 and MCF-7 with lipid vesicles. The level of telomerase activity was found to be significantly (P < .05) downregulated in DEX-treated A-549 and MCF-7 cancer cells. Our results have clearly shown that DEX treatment induces inhibition of cell growth by differentiating into adipocyte-like cells in dexamethasone sensitive cancer cells. |
| first_indexed | 2024-12-12T14:11:31Z |
| format | Article |
| id | doaj.art-17124385fae0457e815f0f2266f57172 |
| institution | Directory Open Access Journal |
| issn | 1976-8354 2151-2485 |
| language | English |
| last_indexed | 2024-12-12T14:11:31Z |
| publishDate | 2018-05-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Animal Cells and Systems |
| spelling | doaj.art-17124385fae0457e815f0f2266f571722022-12-22T00:22:03ZengTaylor & Francis GroupAnimal Cells and Systems1976-83542151-24852018-05-0122317818810.1080/19768354.2018.14764081476408Inhibition of cell growth by cellular differentiation into adipocyte-like cells in dexamethasone sensitive cancer cell linesHea-In Kim0Sun-Ha Moon1Won-Cheol Lee2Hyeon-Jeong Lee3Sharath Belame Shivakumar4Sung-Ho Lee5Bong-Wook Park6Gyu-Jin Rho7Byeong-Gyun Jeon8Gyeongsang National UniversityGyeongsang National UniversityGyeongsang National UniversityGyeongsang National UniversityGyeongsang National UniversityGyeongsang National UniversityChangwon Gyeongsang National University HospitalGyeongsang National UniversityGyeongsang National UniversityThe stress responses in human body lead to secretion of cortisol hormone. The present study investigated the cellular responses on cell growth and cellular differentiation into adipocytes by exposure of synthetic stress hormone, dexamethasone (DEX) in various human cancer and normal cells. After prolonged exposure of cells with 1 μg/ml DEX for 2 weeks, population doubling time (PDT) was significantly (P < .05) increased by inhibited cell growth in A-549 and MCF-7 cancer cells, and was unchanged in MDA-MB-231 cancer cells, normal MRC-5 fibroblasts, umbilical cord matrix-derived mesenchymal stem cells (UCMSCs) and dental papilla tissue-derived mesenchymal stem cells (DSCs). Whereas, PDT was significantly (P < .05) decreased in U87-MG cancer cells by increased cell growth. Glucose uptake was significantly (P < .05) increased in all the cancer cell lines compared to that in normal cell lines. Further, adiposome-like vesicles were noted in A-549 and MCF-7 cancer cells indicating retarded cell growth by DEX treatment, and the vesicles were stained with Oil-Red O solution. Further, the expression of adipocyte-specific genes such as glucose transporter type 4 (GLUT4), glucocorticoid receptors β (GRβ) and peroxisome proliferator-activated receptor γ (PPARγ) were significantly (P < .05) increased in A-549 and MCF-7 with lipid vesicles. The level of telomerase activity was found to be significantly (P < .05) downregulated in DEX-treated A-549 and MCF-7 cancer cells. Our results have clearly shown that DEX treatment induces inhibition of cell growth by differentiating into adipocyte-like cells in dexamethasone sensitive cancer cells.http://dx.doi.org/10.1080/19768354.2018.1476408Humancancerdexamethasonecell differentiationadipocytes |
| spellingShingle | Hea-In Kim Sun-Ha Moon Won-Cheol Lee Hyeon-Jeong Lee Sharath Belame Shivakumar Sung-Ho Lee Bong-Wook Park Gyu-Jin Rho Byeong-Gyun Jeon Inhibition of cell growth by cellular differentiation into adipocyte-like cells in dexamethasone sensitive cancer cell lines Animal Cells and Systems Human cancer dexamethasone cell differentiation adipocytes |
| title | Inhibition of cell growth by cellular differentiation into adipocyte-like cells in dexamethasone sensitive cancer cell lines |
| title_full | Inhibition of cell growth by cellular differentiation into adipocyte-like cells in dexamethasone sensitive cancer cell lines |
| title_fullStr | Inhibition of cell growth by cellular differentiation into adipocyte-like cells in dexamethasone sensitive cancer cell lines |
| title_full_unstemmed | Inhibition of cell growth by cellular differentiation into adipocyte-like cells in dexamethasone sensitive cancer cell lines |
| title_short | Inhibition of cell growth by cellular differentiation into adipocyte-like cells in dexamethasone sensitive cancer cell lines |
| title_sort | inhibition of cell growth by cellular differentiation into adipocyte like cells in dexamethasone sensitive cancer cell lines |
| topic | Human cancer dexamethasone cell differentiation adipocytes |
| url | http://dx.doi.org/10.1080/19768354.2018.1476408 |
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