Autoantigen profiling reveals a shared post-COVID signature in fully recovered and long COVID patients
Some individuals do not return to baseline health following SARS-CoV-2 infection, leading to a condition known as long COVID. The underlying pathophysiology of long COVID remains unknown. Given that autoantibodies have been found to play a role in severity of SARS-CoV-2 infection and certain other p...
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| Format: | Article |
| Language: | English |
| Published: |
American Society for Clinical investigation
2023-06-01
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| Series: | JCI Insight |
| Subjects: | |
| Online Access: | https://doi.org/10.1172/jci.insight.169515 |
| _version_ | 1827767929335185408 |
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| author | Aaron Bodansky Chung-Yu Wang Aditi Saxena Anthea Mitchell Andrew F. Kung Saki Takahashi Khamal Anglin Beatrice Huang Rebecca Hoh Scott Lu Sarah A. Goldberg Justin Romero Brandon Tran Raushun Kirtikar Halle Grebe Matthew So Bryan Greenhouse Matthew S. Durstenfeld Priscilla Y. Hsue Joanna Hellmuth J. Daniel Kelly Jeffrey N. Martin Mark S. Anderson Steven G. Deeks Timothy J. Henrich Joseph L. DeRisi Michael J. Peluso |
| author_facet | Aaron Bodansky Chung-Yu Wang Aditi Saxena Anthea Mitchell Andrew F. Kung Saki Takahashi Khamal Anglin Beatrice Huang Rebecca Hoh Scott Lu Sarah A. Goldberg Justin Romero Brandon Tran Raushun Kirtikar Halle Grebe Matthew So Bryan Greenhouse Matthew S. Durstenfeld Priscilla Y. Hsue Joanna Hellmuth J. Daniel Kelly Jeffrey N. Martin Mark S. Anderson Steven G. Deeks Timothy J. Henrich Joseph L. DeRisi Michael J. Peluso |
| author_sort | Aaron Bodansky |
| collection | DOAJ |
| description | Some individuals do not return to baseline health following SARS-CoV-2 infection, leading to a condition known as long COVID. The underlying pathophysiology of long COVID remains unknown. Given that autoantibodies have been found to play a role in severity of SARS-CoV-2 infection and certain other post-COVID sequelae, their potential role in long COVID is important to investigate. Here, we apply a well-established, unbiased, proteome-wide autoantibody detection technology (T7 phage-display assay with immunoprecipitation and next-generation sequencing, PhIP-Seq) to a robustly phenotyped cohort of 121 individuals with long COVID, 64 individuals with prior COVID-19 who reported full recovery, and 57 pre-COVID controls. While a distinct autoreactive signature was detected that separated individuals with prior SARS-CoV-2 infection from those never exposed to SARS-CoV-2, we did not detect patterns of autoreactivity that separated individuals with long COVID from individuals fully recovered from COVID-19. These data suggest that there are robust alterations in autoreactive antibody profiles due to infection; however, no association of autoreactive antibodies and long COVID was apparent by this assay. |
| first_indexed | 2024-03-11T12:05:20Z |
| format | Article |
| id | doaj.art-1714be604dc64b8c836e37862b1624a6 |
| institution | Directory Open Access Journal |
| issn | 2379-3708 |
| language | English |
| last_indexed | 2024-03-11T12:05:20Z |
| publishDate | 2023-06-01 |
| publisher | American Society for Clinical investigation |
| record_format | Article |
| series | JCI Insight |
| spelling | doaj.art-1714be604dc64b8c836e37862b1624a62023-11-07T16:25:42ZengAmerican Society for Clinical investigationJCI Insight2379-37082023-06-01811Autoantigen profiling reveals a shared post-COVID signature in fully recovered and long COVID patientsAaron BodanskyChung-Yu WangAditi SaxenaAnthea MitchellAndrew F. KungSaki TakahashiKhamal AnglinBeatrice HuangRebecca HohScott LuSarah A. GoldbergJustin RomeroBrandon TranRaushun KirtikarHalle GrebeMatthew SoBryan GreenhouseMatthew S. DurstenfeldPriscilla Y. HsueJoanna HellmuthJ. Daniel KellyJeffrey N. MartinMark S. AndersonSteven G. DeeksTimothy J. HenrichJoseph L. DeRisiMichael J. PelusoSome individuals do not return to baseline health following SARS-CoV-2 infection, leading to a condition known as long COVID. The underlying pathophysiology of long COVID remains unknown. Given that autoantibodies have been found to play a role in severity of SARS-CoV-2 infection and certain other post-COVID sequelae, their potential role in long COVID is important to investigate. Here, we apply a well-established, unbiased, proteome-wide autoantibody detection technology (T7 phage-display assay with immunoprecipitation and next-generation sequencing, PhIP-Seq) to a robustly phenotyped cohort of 121 individuals with long COVID, 64 individuals with prior COVID-19 who reported full recovery, and 57 pre-COVID controls. While a distinct autoreactive signature was detected that separated individuals with prior SARS-CoV-2 infection from those never exposed to SARS-CoV-2, we did not detect patterns of autoreactivity that separated individuals with long COVID from individuals fully recovered from COVID-19. These data suggest that there are robust alterations in autoreactive antibody profiles due to infection; however, no association of autoreactive antibodies and long COVID was apparent by this assay.https://doi.org/10.1172/jci.insight.169515COVID-19 |
| spellingShingle | Aaron Bodansky Chung-Yu Wang Aditi Saxena Anthea Mitchell Andrew F. Kung Saki Takahashi Khamal Anglin Beatrice Huang Rebecca Hoh Scott Lu Sarah A. Goldberg Justin Romero Brandon Tran Raushun Kirtikar Halle Grebe Matthew So Bryan Greenhouse Matthew S. Durstenfeld Priscilla Y. Hsue Joanna Hellmuth J. Daniel Kelly Jeffrey N. Martin Mark S. Anderson Steven G. Deeks Timothy J. Henrich Joseph L. DeRisi Michael J. Peluso Autoantigen profiling reveals a shared post-COVID signature in fully recovered and long COVID patients JCI Insight COVID-19 |
| title | Autoantigen profiling reveals a shared post-COVID signature in fully recovered and long COVID patients |
| title_full | Autoantigen profiling reveals a shared post-COVID signature in fully recovered and long COVID patients |
| title_fullStr | Autoantigen profiling reveals a shared post-COVID signature in fully recovered and long COVID patients |
| title_full_unstemmed | Autoantigen profiling reveals a shared post-COVID signature in fully recovered and long COVID patients |
| title_short | Autoantigen profiling reveals a shared post-COVID signature in fully recovered and long COVID patients |
| title_sort | autoantigen profiling reveals a shared post covid signature in fully recovered and long covid patients |
| topic | COVID-19 |
| url | https://doi.org/10.1172/jci.insight.169515 |
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