Screening for Resistance in Farmer-Preferred Cassava Cultivars from Ghana to a Mixed Infection of CBSV and UCBSV
Cassava brown streak disease (CBSD) caused by the <i>Cassava brown streak virus</i> (CBSV) and <i>Ugandan cassava brown streak virus</i> (UCBSV) is a threat to cassava production in Africa. The potential spread of CBSD into West Africa is a cause for concern, therefore screen...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-08-01
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| Series: | Plants |
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| Online Access: | https://www.mdpi.com/2223-7747/9/8/1026 |
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| author | Wilfred Elegba Wilhelm Gruissem Hervé Vanderschuren |
| author_facet | Wilfred Elegba Wilhelm Gruissem Hervé Vanderschuren |
| author_sort | Wilfred Elegba |
| collection | DOAJ |
| description | Cassava brown streak disease (CBSD) caused by the <i>Cassava brown streak virus</i> (CBSV) and <i>Ugandan cassava brown streak virus</i> (UCBSV) is a threat to cassava production in Africa. The potential spread of CBSD into West Africa is a cause for concern, therefore screening for resistance in farmer-preferred genotypes is crucial for effective control and management. We multiplied a selection of eleven cassava cultivars grown by farmers in Ghana to test their response to a mixed infection of CBSV (TAZ-DES-01) and UCBSV (TAZ-DES-02) isolates using a stringent top-cleft graft inoculation method. Virus titers were quantified in the inoculated scions and cuttings propagated from the inoculated scions to assess virus accumulation and recovery. All cultivars were susceptible to the mixed infection although their response and symptom development varied. In the propagated infected scions, CBSV accumulated at higher titers in leaves of eight of the eleven cultivars. Visual scoring of storage roots from six-month-old virus-inoculated plants revealed the absence of CBSD-associated necrosis symptoms and detectable titers of CBSVs in the cultivar, IFAD. Although all eleven cultivars supported the replication of CBSV and UCBSV in their leaves, the absence of virus replication and CBSD-associated symptoms in the roots of some cultivars could be used as criteria to rapidly advance durable CBSD tolerance using breeding and genetic engineering approaches. |
| first_indexed | 2024-03-10T17:30:21Z |
| format | Article |
| id | doaj.art-1714e0482f8d4b81a2f54e16c6679d56 |
| institution | Directory Open Access Journal |
| issn | 2223-7747 |
| language | English |
| last_indexed | 2024-03-10T17:30:21Z |
| publishDate | 2020-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Plants |
| spelling | doaj.art-1714e0482f8d4b81a2f54e16c6679d562023-11-20T10:01:58ZengMDPI AGPlants2223-77472020-08-0198102610.3390/plants9081026Screening for Resistance in Farmer-Preferred Cassava Cultivars from Ghana to a Mixed Infection of CBSV and UCBSVWilfred Elegba0Wilhelm Gruissem1Hervé Vanderschuren2Plant Biotechnology, Institute of Molecular Plant Biology, Department of Biology, ETH Zurich, 8092 Zurich, SwitzerlandPlant Biotechnology, Institute of Molecular Plant Biology, Department of Biology, ETH Zurich, 8092 Zurich, SwitzerlandPlant Biotechnology, Institute of Molecular Plant Biology, Department of Biology, ETH Zurich, 8092 Zurich, SwitzerlandCassava brown streak disease (CBSD) caused by the <i>Cassava brown streak virus</i> (CBSV) and <i>Ugandan cassava brown streak virus</i> (UCBSV) is a threat to cassava production in Africa. The potential spread of CBSD into West Africa is a cause for concern, therefore screening for resistance in farmer-preferred genotypes is crucial for effective control and management. We multiplied a selection of eleven cassava cultivars grown by farmers in Ghana to test their response to a mixed infection of CBSV (TAZ-DES-01) and UCBSV (TAZ-DES-02) isolates using a stringent top-cleft graft inoculation method. Virus titers were quantified in the inoculated scions and cuttings propagated from the inoculated scions to assess virus accumulation and recovery. All cultivars were susceptible to the mixed infection although their response and symptom development varied. In the propagated infected scions, CBSV accumulated at higher titers in leaves of eight of the eleven cultivars. Visual scoring of storage roots from six-month-old virus-inoculated plants revealed the absence of CBSD-associated necrosis symptoms and detectable titers of CBSVs in the cultivar, IFAD. Although all eleven cultivars supported the replication of CBSV and UCBSV in their leaves, the absence of virus replication and CBSD-associated symptoms in the roots of some cultivars could be used as criteria to rapidly advance durable CBSD tolerance using breeding and genetic engineering approaches.https://www.mdpi.com/2223-7747/9/8/1026cassava brown streak diseaseresistance screeningquantitative reverse transcription PCRtop graft-inoculationvirus detectioncassava germplasm |
| spellingShingle | Wilfred Elegba Wilhelm Gruissem Hervé Vanderschuren Screening for Resistance in Farmer-Preferred Cassava Cultivars from Ghana to a Mixed Infection of CBSV and UCBSV Plants cassava brown streak disease resistance screening quantitative reverse transcription PCR top graft-inoculation virus detection cassava germplasm |
| title | Screening for Resistance in Farmer-Preferred Cassava Cultivars from Ghana to a Mixed Infection of CBSV and UCBSV |
| title_full | Screening for Resistance in Farmer-Preferred Cassava Cultivars from Ghana to a Mixed Infection of CBSV and UCBSV |
| title_fullStr | Screening for Resistance in Farmer-Preferred Cassava Cultivars from Ghana to a Mixed Infection of CBSV and UCBSV |
| title_full_unstemmed | Screening for Resistance in Farmer-Preferred Cassava Cultivars from Ghana to a Mixed Infection of CBSV and UCBSV |
| title_short | Screening for Resistance in Farmer-Preferred Cassava Cultivars from Ghana to a Mixed Infection of CBSV and UCBSV |
| title_sort | screening for resistance in farmer preferred cassava cultivars from ghana to a mixed infection of cbsv and ucbsv |
| topic | cassava brown streak disease resistance screening quantitative reverse transcription PCR top graft-inoculation virus detection cassava germplasm |
| url | https://www.mdpi.com/2223-7747/9/8/1026 |
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