Assessing the skin-whitening property of plant extracts from taiwanese species using zebrafish as a rapid screening platform
Zebrafish can be used as a phenotype-based fast-screening platform for the discovery of skin-whitening products by observing the formation of melanin in vivo through a dissecting microscope. In this study, we first evaluated commercial whitening ingredients to confirm the potency of the zebrafish mo...
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| Format: | Article |
| Language: | English |
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Elsevier
2023-09-01
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| Series: | Arabian Journal of Chemistry |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S1878535223004975 |
| _version_ | 1797776635009695744 |
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| author | Sui-Wen Hsiao I-Chih Kuo Li-Ling Syu Tzong-Huei Lee Chia-Hsiung Cheng Hui-Ching Mei Ching-Kuo Lee |
| author_facet | Sui-Wen Hsiao I-Chih Kuo Li-Ling Syu Tzong-Huei Lee Chia-Hsiung Cheng Hui-Ching Mei Ching-Kuo Lee |
| author_sort | Sui-Wen Hsiao |
| collection | DOAJ |
| description | Zebrafish can be used as a phenotype-based fast-screening platform for the discovery of skin-whitening products by observing the formation of melanin in vivo through a dissecting microscope. In this study, we first evaluated commercial whitening ingredients to confirm the potency of the zebrafish model. The zebrafish screening system verified that verbascoside and 5 other commonly used commercial skin-whitening compounds– including sodium ascorbate, azelaic acid, transamin, kojic acid, and PTU- significantly reduce melanin content in a dose-dependent manner. We subsequently conducted preliminary screening of 123 natural sources to identify those with whitening property. Through a two-step screening process, anti-melanogenesis effects of 4 and 9 candidates at low and high concentration were verified respectively. The toxicity, mortality, and malformation of zebrafish embryo were also evaluated. We further investigated the components of C. manghas L. and found 6 major compounds, one of which was manghaslin, a compound known for its melanin-suppressing effect on zebrafish without cytotoxicity. With these properties, manghaslin is a promising candidate as a skin lightening agent or a treatment for melanoma. |
| first_indexed | 2024-03-12T22:53:39Z |
| format | Article |
| id | doaj.art-17188dcd5d5742aa8b4597af235b5130 |
| institution | Directory Open Access Journal |
| issn | 1878-5352 |
| language | English |
| last_indexed | 2024-03-12T22:53:39Z |
| publishDate | 2023-09-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Arabian Journal of Chemistry |
| spelling | doaj.art-17188dcd5d5742aa8b4597af235b51302023-07-20T04:37:57ZengElsevierArabian Journal of Chemistry1878-53522023-09-01169105035Assessing the skin-whitening property of plant extracts from taiwanese species using zebrafish as a rapid screening platformSui-Wen Hsiao0I-Chih Kuo1Li-Ling Syu2Tzong-Huei Lee3Chia-Hsiung Cheng4Hui-Ching Mei5Ching-Kuo Lee6Ph.D. Program in Drug Discovery and Development Industry, College of Pharmacy, Taipei Medical University, Taipei 11031, TaiwanSchool of Biomedical Engineering, University of British Columbia, Vancouver, BC, CanadaGraduate Institute of Pharmacognosy, Taipei Medical University, Taipei 11031, TaiwanInstitute of Fisheries Science, National Taiwan University, Taipei 10617, TaiwanDepartment of Biochemistry and Molecular Cell Biology, School of Medicine, College of Medicine, Taipei Medical University, Taipei 11031, Taiwan; Corresponding authors at: Ph.D. Program in Drug Discovery and Development Industry, College of Pharmacy, Taipei Medical University, Taipei 11031, Taiwan (C.-K. Lee).Department of Science Education, National Taipei University of Education, Taipei 10671, Taiwan; Corresponding authors at: Ph.D. Program in Drug Discovery and Development Industry, College of Pharmacy, Taipei Medical University, Taipei 11031, Taiwan (C.-K. Lee).Ph.D. Program in Drug Discovery and Development Industry, College of Pharmacy, Taipei Medical University, Taipei 11031, Taiwan; Graduate Institute of Pharmacognosy, Taipei Medical University, Taipei 11031, Taiwan; School of Pharmacy, Taipei Medical University, Taipei 11031, Taiwan; Corresponding authors at: Ph.D. Program in Drug Discovery and Development Industry, College of Pharmacy, Taipei Medical University, Taipei 11031, Taiwan (C.-K. Lee).Zebrafish can be used as a phenotype-based fast-screening platform for the discovery of skin-whitening products by observing the formation of melanin in vivo through a dissecting microscope. In this study, we first evaluated commercial whitening ingredients to confirm the potency of the zebrafish model. The zebrafish screening system verified that verbascoside and 5 other commonly used commercial skin-whitening compounds– including sodium ascorbate, azelaic acid, transamin, kojic acid, and PTU- significantly reduce melanin content in a dose-dependent manner. We subsequently conducted preliminary screening of 123 natural sources to identify those with whitening property. Through a two-step screening process, anti-melanogenesis effects of 4 and 9 candidates at low and high concentration were verified respectively. The toxicity, mortality, and malformation of zebrafish embryo were also evaluated. We further investigated the components of C. manghas L. and found 6 major compounds, one of which was manghaslin, a compound known for its melanin-suppressing effect on zebrafish without cytotoxicity. With these properties, manghaslin is a promising candidate as a skin lightening agent or a treatment for melanoma.http://www.sciencedirect.com/science/article/pii/S1878535223004975Zebrafish (Danio rerio)WhiteningPhenotype-based screeningNatural extractsCerbera manghasManghaslin |
| spellingShingle | Sui-Wen Hsiao I-Chih Kuo Li-Ling Syu Tzong-Huei Lee Chia-Hsiung Cheng Hui-Ching Mei Ching-Kuo Lee Assessing the skin-whitening property of plant extracts from taiwanese species using zebrafish as a rapid screening platform Arabian Journal of Chemistry Zebrafish (Danio rerio) Whitening Phenotype-based screening Natural extracts Cerbera manghas Manghaslin |
| title | Assessing the skin-whitening property of plant extracts from taiwanese species using zebrafish as a rapid screening platform |
| title_full | Assessing the skin-whitening property of plant extracts from taiwanese species using zebrafish as a rapid screening platform |
| title_fullStr | Assessing the skin-whitening property of plant extracts from taiwanese species using zebrafish as a rapid screening platform |
| title_full_unstemmed | Assessing the skin-whitening property of plant extracts from taiwanese species using zebrafish as a rapid screening platform |
| title_short | Assessing the skin-whitening property of plant extracts from taiwanese species using zebrafish as a rapid screening platform |
| title_sort | assessing the skin whitening property of plant extracts from taiwanese species using zebrafish as a rapid screening platform |
| topic | Zebrafish (Danio rerio) Whitening Phenotype-based screening Natural extracts Cerbera manghas Manghaslin |
| url | http://www.sciencedirect.com/science/article/pii/S1878535223004975 |
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