Virus-induced opposite effect on Bombyx mori gene transcriptions
Bombyx mori bidensovirus (BmBDV) and Bombyx mori nucleopolyhedrovirus (BmNPV) are serious pathogens of Bombyx mori. In this study, we reported the changes of transcription level of several immune genes, including bmi, argo, dicer, cap1, cap3 and car, in Bombyx mori midgut after exposure to BmBDV...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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University of Modena and Reggio Emilia
2016-09-01
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| Series: | Invertebrate Survival Journal |
| Subjects: | |
| Online Access: | http://www.isj.unimo.it/articoli/ISJ436.pdf |
| _version_ | 1828776422035947520 |
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| author | Y Yin H Xia F Zhu L Chen P Lü K Chen |
| author_facet | Y Yin H Xia F Zhu L Chen P Lü K Chen |
| author_sort | Y Yin |
| collection | DOAJ |
| description | Bombyx mori bidensovirus (BmBDV) and Bombyx mori nucleopolyhedrovirus (BmNPV) are serious
pathogens of Bombyx mori. In this study, we reported the changes of transcription level of several
immune genes, including bmi, argo, dicer, cap1, cap3 and car, in Bombyx mori midgut after exposure to
BmBDV or BmNPV. Silkworm strains 798 (anti-BmBDV) and 306 (susceptible to BmBDV) were
subjected to BmBDV infection, and NB (anti-BmNPV) and HUABA (35) (susceptible to BmNPV) were
subjected to BmNPV infection. The results showed that the transcription levels differ largely among
different silkworm strains, and that the extent to which the gene transcriptions were affected by the
viruses was different. However, both BmNPV and BmBDV viruses can reverse the transcription
patterns of these genes when the silkworms were administered with the viruses compared with those
control groups. The transcript levels of bmi and dicer were decreased in 798 and 306 strains that were
inoculated with BmBDV compared with their respective controls, but were increased in NB and HUABA
(35) inoculated with BmNPV. The transcript levels of argo and cap3 were risen in 798, 306 and NB
strains when inoculated with their respective viruses, but were decreased in HUABA (35) strain. The
transcript levels of cap1 were risen in all silkworm strains, while the levels of car were decreased in 798,
306 and HUABA (35) strains, and increased in NB strain when inoculated with their respective viruses.
These findings may contribute to more in-depth understanding on functions of these genes in virus
infection and proliferation. |
| first_indexed | 2024-12-11T15:58:21Z |
| format | Article |
| id | doaj.art-171b75d05fb54299ac5683a36bc3b43d |
| institution | Directory Open Access Journal |
| issn | 1824-307X |
| language | English |
| last_indexed | 2024-12-11T15:58:21Z |
| publishDate | 2016-09-01 |
| publisher | University of Modena and Reggio Emilia |
| record_format | Article |
| series | Invertebrate Survival Journal |
| spelling | doaj.art-171b75d05fb54299ac5683a36bc3b43d2022-12-22T00:59:22ZengUniversity of Modena and Reggio EmiliaInvertebrate Survival Journal1824-307X2016-09-0113291297Virus-induced opposite effect on Bombyx mori gene transcriptionsY Yin0H Xia1F Zhu2L Chen3P Lü4K Chen5Institute of Life Sciences, Jiangsu University, Zhenjiang, 212013, Jiangsu, P. R. ChinaInstitute of Life Sciences, Jiangsu University, Zhenjiang, 212013, Jiangsu, P. R. ChinaInstitute of Life Sciences, Jiangsu University, Zhenjiang, 212013, Jiangsu, P. R. ChinaInstitute of Life Sciences, Jiangsu University, Zhenjiang, 212013, Jiangsu, P. R. ChinaInstitute of Life Sciences, Jiangsu University, Zhenjiang, 212013, Jiangsu, P. R. ChinaInstitute of Life Sciences, Jiangsu University, Zhenjiang, 212013, Jiangsu, P. R. ChinaBombyx mori bidensovirus (BmBDV) and Bombyx mori nucleopolyhedrovirus (BmNPV) are serious pathogens of Bombyx mori. In this study, we reported the changes of transcription level of several immune genes, including bmi, argo, dicer, cap1, cap3 and car, in Bombyx mori midgut after exposure to BmBDV or BmNPV. Silkworm strains 798 (anti-BmBDV) and 306 (susceptible to BmBDV) were subjected to BmBDV infection, and NB (anti-BmNPV) and HUABA (35) (susceptible to BmNPV) were subjected to BmNPV infection. The results showed that the transcription levels differ largely among different silkworm strains, and that the extent to which the gene transcriptions were affected by the viruses was different. However, both BmNPV and BmBDV viruses can reverse the transcription patterns of these genes when the silkworms were administered with the viruses compared with those control groups. The transcript levels of bmi and dicer were decreased in 798 and 306 strains that were inoculated with BmBDV compared with their respective controls, but were increased in NB and HUABA (35) inoculated with BmNPV. The transcript levels of argo and cap3 were risen in 798, 306 and NB strains when inoculated with their respective viruses, but were decreased in HUABA (35) strain. The transcript levels of cap1 were risen in all silkworm strains, while the levels of car were decreased in 798, 306 and HUABA (35) strains, and increased in NB strain when inoculated with their respective viruses. These findings may contribute to more in-depth understanding on functions of these genes in virus infection and proliferation.http://www.isj.unimo.it/articoli/ISJ436.pdfBmBDV; BmNPV; Bombyx mori; qPCR; immune genes; expression level |
| spellingShingle | Y Yin H Xia F Zhu L Chen P Lü K Chen Virus-induced opposite effect on Bombyx mori gene transcriptions Invertebrate Survival Journal BmBDV; BmNPV; Bombyx mori; qPCR; immune genes; expression level |
| title | Virus-induced opposite effect on Bombyx mori gene transcriptions |
| title_full | Virus-induced opposite effect on Bombyx mori gene transcriptions |
| title_fullStr | Virus-induced opposite effect on Bombyx mori gene transcriptions |
| title_full_unstemmed | Virus-induced opposite effect on Bombyx mori gene transcriptions |
| title_short | Virus-induced opposite effect on Bombyx mori gene transcriptions |
| title_sort | virus induced opposite effect on bombyx mori gene transcriptions |
| topic | BmBDV; BmNPV; Bombyx mori; qPCR; immune genes; expression level |
| url | http://www.isj.unimo.it/articoli/ISJ436.pdf |
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