MircroRNA Profiles of Early Rice Inflorescence Revealed a Specific miRNA5506 Regulating Development of Floral Organs and Female Megagametophyte in Rice
The developmental process of inflorescence and gametophytes is vital for sexual reproduction in rice. Multiple genes and conserved miRNAs have been characterized to regulate the process. The changes of miRNAs expression during the early development of rice inflorescence remain unknown. In this study...
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MDPI AG
2021-06-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/12/6610 |
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| author | Zhixiong Chen Yajing Li Peigang Li Xiaojie Huang Mingxin Chen Jinwen Wu Lang Wang Xiangdong Liu Yajuan Li |
| author_facet | Zhixiong Chen Yajing Li Peigang Li Xiaojie Huang Mingxin Chen Jinwen Wu Lang Wang Xiangdong Liu Yajuan Li |
| author_sort | Zhixiong Chen |
| collection | DOAJ |
| description | The developmental process of inflorescence and gametophytes is vital for sexual reproduction in rice. Multiple genes and conserved miRNAs have been characterized to regulate the process. The changes of miRNAs expression during the early development of rice inflorescence remain unknown. In this study, the analysis of miRNAs profiles in the early stage of rice inflorescence development identified 671 miRNAs, including 67 known and 44 novel differentially expressed miRNAs (DEMs). Six distinct clusters of miRNAs expression patterns were detected, and Cluster 5 comprised 110 DEMs, including unconserved, rice-specific osa-miR5506. Overexpression of osa-miR5506 caused pleiotropic abnormalities, including over- or under-developed palea, various numbers of floral organs and spikelet indeterminacy. In addition, the defects of ovaries development were frequently characterized by multiple megasporocytes, ovule-free ovary, megasporocyte degenerated and embryo sac degenerated in the transgenic lines. osa-miR5506 targeted REM transcription factor <i>LOC_Os03g11370</i>. Summarily, these results demonstrated that rice-specific osa-miR5506 plays an essential role in the regulation of floral organ number, spikelet determinacy and female gametophyte development in rice. |
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| id | doaj.art-171e2b0adc0648b1b4ec6fdb8359c6c4 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T10:13:19Z |
| publishDate | 2021-06-01 |
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| series | International Journal of Molecular Sciences |
| spelling | doaj.art-171e2b0adc0648b1b4ec6fdb8359c6c42023-11-22T00:58:48ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-06-012212661010.3390/ijms22126610MircroRNA Profiles of Early Rice Inflorescence Revealed a Specific miRNA5506 Regulating Development of Floral Organs and Female Megagametophyte in RiceZhixiong Chen0Yajing Li1Peigang Li2Xiaojie Huang3Mingxin Chen4Jinwen Wu5Lang Wang6Xiangdong Liu7Yajuan Li8State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou 510642, ChinaDepartment of Plant Breeding, College of Agriculture, South China Agricultural University, Guangzhou 510642, ChinaDepartment of Plant Breeding, College of Agriculture, South China Agricultural University, Guangzhou 510642, ChinaDepartment of Plant Breeding, College of Agriculture, South China Agricultural University, Guangzhou 510642, ChinaDepartment of Plant Breeding, College of Agriculture, South China Agricultural University, Guangzhou 510642, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou 510642, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou 510642, ChinaState Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, South China Agricultural University, Guangzhou 510642, ChinaCenter of Experimental Teaching for Common Basic Courses, South China Agricultural University, Guangzhou 510642, ChinaThe developmental process of inflorescence and gametophytes is vital for sexual reproduction in rice. Multiple genes and conserved miRNAs have been characterized to regulate the process. The changes of miRNAs expression during the early development of rice inflorescence remain unknown. In this study, the analysis of miRNAs profiles in the early stage of rice inflorescence development identified 671 miRNAs, including 67 known and 44 novel differentially expressed miRNAs (DEMs). Six distinct clusters of miRNAs expression patterns were detected, and Cluster 5 comprised 110 DEMs, including unconserved, rice-specific osa-miR5506. Overexpression of osa-miR5506 caused pleiotropic abnormalities, including over- or under-developed palea, various numbers of floral organs and spikelet indeterminacy. In addition, the defects of ovaries development were frequently characterized by multiple megasporocytes, ovule-free ovary, megasporocyte degenerated and embryo sac degenerated in the transgenic lines. osa-miR5506 targeted REM transcription factor <i>LOC_Os03g11370</i>. Summarily, these results demonstrated that rice-specific osa-miR5506 plays an essential role in the regulation of floral organ number, spikelet determinacy and female gametophyte development in rice.https://www.mdpi.com/1422-0067/22/12/6610ricefloral developmentmiRNAembryo sacfemale gametophyte |
| spellingShingle | Zhixiong Chen Yajing Li Peigang Li Xiaojie Huang Mingxin Chen Jinwen Wu Lang Wang Xiangdong Liu Yajuan Li MircroRNA Profiles of Early Rice Inflorescence Revealed a Specific miRNA5506 Regulating Development of Floral Organs and Female Megagametophyte in Rice International Journal of Molecular Sciences rice floral development miRNA embryo sac female gametophyte |
| title | MircroRNA Profiles of Early Rice Inflorescence Revealed a Specific miRNA5506 Regulating Development of Floral Organs and Female Megagametophyte in Rice |
| title_full | MircroRNA Profiles of Early Rice Inflorescence Revealed a Specific miRNA5506 Regulating Development of Floral Organs and Female Megagametophyte in Rice |
| title_fullStr | MircroRNA Profiles of Early Rice Inflorescence Revealed a Specific miRNA5506 Regulating Development of Floral Organs and Female Megagametophyte in Rice |
| title_full_unstemmed | MircroRNA Profiles of Early Rice Inflorescence Revealed a Specific miRNA5506 Regulating Development of Floral Organs and Female Megagametophyte in Rice |
| title_short | MircroRNA Profiles of Early Rice Inflorescence Revealed a Specific miRNA5506 Regulating Development of Floral Organs and Female Megagametophyte in Rice |
| title_sort | mircrorna profiles of early rice inflorescence revealed a specific mirna5506 regulating development of floral organs and female megagametophyte in rice |
| topic | rice floral development miRNA embryo sac female gametophyte |
| url | https://www.mdpi.com/1422-0067/22/12/6610 |
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