Ctotoxic and apoptogenic effects of Perovskia abrotanoides flower extract on MCF-7 and HeLa cell lines
Objective: Perovskia abrotanoides Karel, belongs to the family Lamiaceae and grows wild alongside the mountainous roads inarid and cold climate of Northern Iran. The anti-tumor activity of P. abrotanoides root extract has been shown previously. This study was designed to examine in vitro anti-prolif...
| Main Authors: | , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Mashhad University of Medical Sciences
2016-06-01
|
| Series: | Avicenna Journal of Phytomedicine |
| Subjects: | |
| Online Access: | http://ajp.mums.ac.ir/article_6263_230bf2d1ce8678f7e5684bf83ca7ee5f.pdf |
| _version_ | 1818057687552753664 |
|---|---|
| author | Mohamad Ali Geryani Davood Mahdian Seyed Hadi Mousavi Azar Hosseini |
| author_facet | Mohamad Ali Geryani Davood Mahdian Seyed Hadi Mousavi Azar Hosseini |
| author_sort | Mohamad Ali Geryani |
| collection | DOAJ |
| description | Objective: Perovskia abrotanoides Karel, belongs to the family Lamiaceae and grows wild alongside the mountainous roads inarid and cold climate of Northern Iran. The anti-tumor activity of P. abrotanoides root extract has been shown previously. This study was designed to examine in vitro anti-proliferative and pro-apoptotic effects of flower extract of P. abrotanoides on MCF-7 and Hela cell lines. Materials and Methods: Cells were cultured in DMEM medium with 10% fetal bovine serum, 100 units/ml penicillin and 100 µg/ml streptomycin and incubated with different concentrations of plant extracts. Cell viability was quantified by MTT assay. Apoptotic cells were determined using propidium iodide (PI) staining of DNA fragmentation by flow cytometry (sub-G1 peak). Results: P. abrotanoides extract inhibited the growth of malignant cells in a time and dose-dependent manner and 1000 µg/ml of extract following 48h of incubation was the most cytotoxic dose against Hela cell in comparison with other doses; however, in MCF-7 cells,1000 and 500 µg/ml PA induced toxicity at all time points but with different features.. Analysis of flowcytometry histogram of treated cells compared with control cells indicated that the cytotoxic effect is partly due toapoptosis induction. Conclusion: Hydro-alcoholic extract of P. abrotanoides flowers inhibits the growth of MCF-7 and HeLa cell lines, partly via inducing apoptosis. Their inhibitory effect was increased in a time and dose-dependent manner, especially in MCF7 cells. However, further studies are needed to reveal the mechanisms of P. abrotanoides extract-induced cell death. |
| first_indexed | 2024-12-10T12:48:42Z |
| format | Article |
| id | doaj.art-171e9a0e531944b9b16fe31c2c1eb64a |
| institution | Directory Open Access Journal |
| issn | 2228-7930 2228-7949 |
| language | English |
| last_indexed | 2024-12-10T12:48:42Z |
| publishDate | 2016-06-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Avicenna Journal of Phytomedicine |
| spelling | doaj.art-171e9a0e531944b9b16fe31c2c1eb64a2022-12-22T01:48:19ZengMashhad University of Medical SciencesAvicenna Journal of Phytomedicine2228-79302228-79492016-06-01644104176263Ctotoxic and apoptogenic effects of Perovskia abrotanoides flower extract on MCF-7 and HeLa cell linesMohamad Ali Geryani0Davood Mahdian1Seyed Hadi Mousavi2Azar Hosseini3Department of Pharmacology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, IranDepartment of Pharmacology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, IranPharmacological Research Center of Medicinal Plants, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.Pharmacological Research Center of Medicinal Plants, School of Medicine, Mashhad University of Medical Sciences, Mashhad, IranObjective: Perovskia abrotanoides Karel, belongs to the family Lamiaceae and grows wild alongside the mountainous roads inarid and cold climate of Northern Iran. The anti-tumor activity of P. abrotanoides root extract has been shown previously. This study was designed to examine in vitro anti-proliferative and pro-apoptotic effects of flower extract of P. abrotanoides on MCF-7 and Hela cell lines. Materials and Methods: Cells were cultured in DMEM medium with 10% fetal bovine serum, 100 units/ml penicillin and 100 µg/ml streptomycin and incubated with different concentrations of plant extracts. Cell viability was quantified by MTT assay. Apoptotic cells were determined using propidium iodide (PI) staining of DNA fragmentation by flow cytometry (sub-G1 peak). Results: P. abrotanoides extract inhibited the growth of malignant cells in a time and dose-dependent manner and 1000 µg/ml of extract following 48h of incubation was the most cytotoxic dose against Hela cell in comparison with other doses; however, in MCF-7 cells,1000 and 500 µg/ml PA induced toxicity at all time points but with different features.. Analysis of flowcytometry histogram of treated cells compared with control cells indicated that the cytotoxic effect is partly due toapoptosis induction. Conclusion: Hydro-alcoholic extract of P. abrotanoides flowers inhibits the growth of MCF-7 and HeLa cell lines, partly via inducing apoptosis. Their inhibitory effect was increased in a time and dose-dependent manner, especially in MCF7 cells. However, further studies are needed to reveal the mechanisms of P. abrotanoides extract-induced cell death.http://ajp.mums.ac.ir/article_6263_230bf2d1ce8678f7e5684bf83ca7ee5f.pdfPerovskia abrotanoidesbreast cancerCervical cancerApoptosisMCF-7Hela |
| spellingShingle | Mohamad Ali Geryani Davood Mahdian Seyed Hadi Mousavi Azar Hosseini Ctotoxic and apoptogenic effects of Perovskia abrotanoides flower extract on MCF-7 and HeLa cell lines Avicenna Journal of Phytomedicine Perovskia abrotanoides breast cancer Cervical cancer Apoptosis MCF-7 Hela |
| title | Ctotoxic and apoptogenic effects of Perovskia abrotanoides flower extract on MCF-7 and HeLa cell lines |
| title_full | Ctotoxic and apoptogenic effects of Perovskia abrotanoides flower extract on MCF-7 and HeLa cell lines |
| title_fullStr | Ctotoxic and apoptogenic effects of Perovskia abrotanoides flower extract on MCF-7 and HeLa cell lines |
| title_full_unstemmed | Ctotoxic and apoptogenic effects of Perovskia abrotanoides flower extract on MCF-7 and HeLa cell lines |
| title_short | Ctotoxic and apoptogenic effects of Perovskia abrotanoides flower extract on MCF-7 and HeLa cell lines |
| title_sort | ctotoxic and apoptogenic effects of perovskia abrotanoides flower extract on mcf 7 and hela cell lines |
| topic | Perovskia abrotanoides breast cancer Cervical cancer Apoptosis MCF-7 Hela |
| url | http://ajp.mums.ac.ir/article_6263_230bf2d1ce8678f7e5684bf83ca7ee5f.pdf |
| work_keys_str_mv | AT mohamadaligeryani ctotoxicandapoptogeniceffectsofperovskiaabrotanoidesflowerextractonmcf7andhelacelllines AT davoodmahdian ctotoxicandapoptogeniceffectsofperovskiaabrotanoidesflowerextractonmcf7andhelacelllines AT seyedhadimousavi ctotoxicandapoptogeniceffectsofperovskiaabrotanoidesflowerextractonmcf7andhelacelllines AT azarhosseini ctotoxicandapoptogeniceffectsofperovskiaabrotanoidesflowerextractonmcf7andhelacelllines |