The relationship between obesity-related H19DMR methylation and H19 and IGF2 gene expression on offspring growth and body composition
Background and objectiveImprinted genes are important for the offspring development. To assess the relationship between obesity-related H19DMR methylation and H19 and IGF2 gene expression and offspring growth and body composition.MethodsThirty-nine overweight/obese and 25 normal weight pregnant wome...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Frontiers Media S.A.
2023-09-01
|
| Series: | Frontiers in Nutrition |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fnut.2023.1170411/full |
| _version_ | 1797678004663484416 |
|---|---|
| author | Perla Pizzi Argentato Jorge Augusto Petroli Marchesi Naiara Naiana Dejani Patrícia Yury Nakandakare Laísla de França da Silva Teles Lívia Patrícia Rodrigues Batista Maria Paula Carvalho Leitão Liania Alves Luzia Ester Silveira Ramos Patricia Helen Rondó |
| author_facet | Perla Pizzi Argentato Jorge Augusto Petroli Marchesi Naiara Naiana Dejani Patrícia Yury Nakandakare Laísla de França da Silva Teles Lívia Patrícia Rodrigues Batista Maria Paula Carvalho Leitão Liania Alves Luzia Ester Silveira Ramos Patricia Helen Rondó |
| author_sort | Perla Pizzi Argentato |
| collection | DOAJ |
| description | Background and objectiveImprinted genes are important for the offspring development. To assess the relationship between obesity-related H19DMR methylation and H19 and IGF2 gene expression and offspring growth and body composition.MethodsThirty-nine overweight/obese and 25 normal weight pregnant women were selected from the “Araraquara Cohort Study” according to their pre-pregnancy BMI. Fetal growth and body composition and newborn growth were assessed, respectively, by ultrasound and anthropometry. The methylation of H19DMR in maternal blood, cord blood, maternal decidua and placental villi tissues was evaluated by methylation-sensitive restriction endonuclease qPCR, and H19 and IGF2 expression by relative real-time PCR quantification. Multiple linear regression models explored the associations of DNA methylation and gene expression with maternal, fetal, and newborn parameters.ResultsH19DMR was less methylated in maternal blood of the overweight/obese group. There were associations of H19DMR methylation in cord blood with centiles of fetal biparietal diameter (BPD) and abdominal subcutaneous fat thickness and newborn head circumference (HC); H19DMR methylation in maternal decidua with fetal occipitofrontal diameter (OFD), HC, and length; H19DMR methylation in placental villi with fetal OFD, HC and abdominal subcutaneous fat thickness and with newborn HC. H19 expression in maternal decidua was associated with fetal BPD and femur length centiles and in placental villi with fetal OFD and subcutaneous arm fat. IGF2 expression in maternal decidua was associated with fetal BPD and in placental villi with fetal OFD.ConclusionTo our knowledge, this is the first study to demonstrate associations of imprinted genes variations at the maternal-fetal interface of the placenta and in cord blood with fetal body composition, supporting the involvement of epigenetic mechanisms in offspring growth and body composition. |
| first_indexed | 2024-03-11T22:54:00Z |
| format | Article |
| id | doaj.art-1720963b2f174ce2af995726a8b3e7a2 |
| institution | Directory Open Access Journal |
| issn | 2296-861X |
| language | English |
| last_indexed | 2024-03-11T22:54:00Z |
| publishDate | 2023-09-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Nutrition |
| spelling | doaj.art-1720963b2f174ce2af995726a8b3e7a22023-09-21T19:19:12ZengFrontiers Media S.A.Frontiers in Nutrition2296-861X2023-09-011010.3389/fnut.2023.11704111170411The relationship between obesity-related H19DMR methylation and H19 and IGF2 gene expression on offspring growth and body compositionPerla Pizzi Argentato0Jorge Augusto Petroli Marchesi1Naiara Naiana Dejani2Patrícia Yury Nakandakare3Laísla de França da Silva Teles4Lívia Patrícia Rodrigues Batista5Maria Paula Carvalho Leitão6Liania Alves Luzia7Ester Silveira Ramos8Patricia Helen Rondó9Nutrition Department, School of Public Health, University of São Paulo, São Paulo, BrazilDepartment of Genetics, Ribeirão Preto Medical School, University of São Paulo, São Paulo, BrazilNutrition Department, School of Public Health, University of São Paulo, São Paulo, BrazilNutrition Department, School of Public Health, University of São Paulo, São Paulo, BrazilNutrition Department, School of Public Health, University of São Paulo, São Paulo, BrazilNutrition Department, School of Public Health, University of São Paulo, São Paulo, BrazilNutrition Department, School of Public Health, University of São Paulo, São Paulo, BrazilNutrition Department, School of Public Health, University of São Paulo, São Paulo, BrazilDepartment of Genetics, Ribeirão Preto Medical School, University of São Paulo, São Paulo, BrazilNutrition Department, School of Public Health, University of São Paulo, São Paulo, BrazilBackground and objectiveImprinted genes are important for the offspring development. To assess the relationship between obesity-related H19DMR methylation and H19 and IGF2 gene expression and offspring growth and body composition.MethodsThirty-nine overweight/obese and 25 normal weight pregnant women were selected from the “Araraquara Cohort Study” according to their pre-pregnancy BMI. Fetal growth and body composition and newborn growth were assessed, respectively, by ultrasound and anthropometry. The methylation of H19DMR in maternal blood, cord blood, maternal decidua and placental villi tissues was evaluated by methylation-sensitive restriction endonuclease qPCR, and H19 and IGF2 expression by relative real-time PCR quantification. Multiple linear regression models explored the associations of DNA methylation and gene expression with maternal, fetal, and newborn parameters.ResultsH19DMR was less methylated in maternal blood of the overweight/obese group. There were associations of H19DMR methylation in cord blood with centiles of fetal biparietal diameter (BPD) and abdominal subcutaneous fat thickness and newborn head circumference (HC); H19DMR methylation in maternal decidua with fetal occipitofrontal diameter (OFD), HC, and length; H19DMR methylation in placental villi with fetal OFD, HC and abdominal subcutaneous fat thickness and with newborn HC. H19 expression in maternal decidua was associated with fetal BPD and femur length centiles and in placental villi with fetal OFD and subcutaneous arm fat. IGF2 expression in maternal decidua was associated with fetal BPD and in placental villi with fetal OFD.ConclusionTo our knowledge, this is the first study to demonstrate associations of imprinted genes variations at the maternal-fetal interface of the placenta and in cord blood with fetal body composition, supporting the involvement of epigenetic mechanisms in offspring growth and body composition.https://www.frontiersin.org/articles/10.3389/fnut.2023.1170411/fullmaternal obesityfetal growthfetal body compositionimprinted geneDNA methylationcord blood |
| spellingShingle | Perla Pizzi Argentato Jorge Augusto Petroli Marchesi Naiara Naiana Dejani Patrícia Yury Nakandakare Laísla de França da Silva Teles Lívia Patrícia Rodrigues Batista Maria Paula Carvalho Leitão Liania Alves Luzia Ester Silveira Ramos Patricia Helen Rondó The relationship between obesity-related H19DMR methylation and H19 and IGF2 gene expression on offspring growth and body composition Frontiers in Nutrition maternal obesity fetal growth fetal body composition imprinted gene DNA methylation cord blood |
| title | The relationship between obesity-related H19DMR methylation and H19 and IGF2 gene expression on offspring growth and body composition |
| title_full | The relationship between obesity-related H19DMR methylation and H19 and IGF2 gene expression on offspring growth and body composition |
| title_fullStr | The relationship between obesity-related H19DMR methylation and H19 and IGF2 gene expression on offspring growth and body composition |
| title_full_unstemmed | The relationship between obesity-related H19DMR methylation and H19 and IGF2 gene expression on offspring growth and body composition |
| title_short | The relationship between obesity-related H19DMR methylation and H19 and IGF2 gene expression on offspring growth and body composition |
| title_sort | relationship between obesity related h19dmr methylation and h19 and igf2 gene expression on offspring growth and body composition |
| topic | maternal obesity fetal growth fetal body composition imprinted gene DNA methylation cord blood |
| url | https://www.frontiersin.org/articles/10.3389/fnut.2023.1170411/full |
| work_keys_str_mv | AT perlapizziargentato therelationshipbetweenobesityrelatedh19dmrmethylationandh19andigf2geneexpressiononoffspringgrowthandbodycomposition AT jorgeaugustopetrolimarchesi therelationshipbetweenobesityrelatedh19dmrmethylationandh19andigf2geneexpressiononoffspringgrowthandbodycomposition AT naiaranaianadejani therelationshipbetweenobesityrelatedh19dmrmethylationandh19andigf2geneexpressiononoffspringgrowthandbodycomposition AT patriciayurynakandakare therelationshipbetweenobesityrelatedh19dmrmethylationandh19andigf2geneexpressiononoffspringgrowthandbodycomposition AT laisladefrancadasilvateles therelationshipbetweenobesityrelatedh19dmrmethylationandh19andigf2geneexpressiononoffspringgrowthandbodycomposition AT liviapatriciarodriguesbatista therelationshipbetweenobesityrelatedh19dmrmethylationandh19andigf2geneexpressiononoffspringgrowthandbodycomposition AT mariapaulacarvalholeitao therelationshipbetweenobesityrelatedh19dmrmethylationandh19andigf2geneexpressiononoffspringgrowthandbodycomposition AT lianiaalvesluzia therelationshipbetweenobesityrelatedh19dmrmethylationandh19andigf2geneexpressiononoffspringgrowthandbodycomposition AT estersilveiraramos therelationshipbetweenobesityrelatedh19dmrmethylationandh19andigf2geneexpressiononoffspringgrowthandbodycomposition AT patriciahelenrondo therelationshipbetweenobesityrelatedh19dmrmethylationandh19andigf2geneexpressiononoffspringgrowthandbodycomposition AT perlapizziargentato relationshipbetweenobesityrelatedh19dmrmethylationandh19andigf2geneexpressiononoffspringgrowthandbodycomposition AT jorgeaugustopetrolimarchesi relationshipbetweenobesityrelatedh19dmrmethylationandh19andigf2geneexpressiononoffspringgrowthandbodycomposition AT naiaranaianadejani relationshipbetweenobesityrelatedh19dmrmethylationandh19andigf2geneexpressiononoffspringgrowthandbodycomposition AT patriciayurynakandakare relationshipbetweenobesityrelatedh19dmrmethylationandh19andigf2geneexpressiononoffspringgrowthandbodycomposition AT laisladefrancadasilvateles relationshipbetweenobesityrelatedh19dmrmethylationandh19andigf2geneexpressiononoffspringgrowthandbodycomposition AT liviapatriciarodriguesbatista relationshipbetweenobesityrelatedh19dmrmethylationandh19andigf2geneexpressiononoffspringgrowthandbodycomposition AT mariapaulacarvalholeitao relationshipbetweenobesityrelatedh19dmrmethylationandh19andigf2geneexpressiononoffspringgrowthandbodycomposition AT lianiaalvesluzia relationshipbetweenobesityrelatedh19dmrmethylationandh19andigf2geneexpressiononoffspringgrowthandbodycomposition AT estersilveiraramos relationshipbetweenobesityrelatedh19dmrmethylationandh19andigf2geneexpressiononoffspringgrowthandbodycomposition AT patriciahelenrondo relationshipbetweenobesityrelatedh19dmrmethylationandh19andigf2geneexpressiononoffspringgrowthandbodycomposition |