Links between Soil Bacteriobiomes and Fungistasis toward Fungi Infecting the Colorado Potato Beetle
Entomopathogenic fungi can be inhibited by different soil microorganisms, but the effect of a soil microbiota on fungal growth, survival, and infectivity toward insects is insufficiently understood. We investigated the level of fungistasis toward <i>Metarhizium robertsii</i> and <i>...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-04-01
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| Series: | Microorganisms |
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| Online Access: | https://www.mdpi.com/2076-2607/11/4/943 |
| _version_ | 1797604220315107328 |
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| author | Ekaterina Chertkova Marsel R. Kabilov Olga Yaroslavtseva Olga Polenogova Elena Kosman Darya Sidorenko Tatyana Alikina Yury Noskov Anton Krivopalov Viktor V. Glupov Vadim Yu. Kryukov |
| author_facet | Ekaterina Chertkova Marsel R. Kabilov Olga Yaroslavtseva Olga Polenogova Elena Kosman Darya Sidorenko Tatyana Alikina Yury Noskov Anton Krivopalov Viktor V. Glupov Vadim Yu. Kryukov |
| author_sort | Ekaterina Chertkova |
| collection | DOAJ |
| description | Entomopathogenic fungi can be inhibited by different soil microorganisms, but the effect of a soil microbiota on fungal growth, survival, and infectivity toward insects is insufficiently understood. We investigated the level of fungistasis toward <i>Metarhizium robertsii</i> and <i>Beauveria bassiana</i> in soils of conventional potato fields and kitchen potato gardens. Agar diffusion methods, 16S rDNA metabarcoding, bacterial DNA quantification, and assays of <i>Leptinotarsa decemlineata</i> survival in soils inoculated with fungal conidia were used. Soils of kitchen gardens showed stronger fungistasis toward <i>M. robertsii</i> and <i>B. bassiana</i> and at the same time the highest density of the fungi compared to soils of conventional fields. The fungistasis level depended on the quantity of bacterial DNA and relative abundance of <i>Bacillus</i>, <i>Streptomyces</i>, and some <i>Proteobacteria</i>, whose abundance levels were the highest in kitchen garden soils. Cultivable isolates of bacilli exhibited antagonism to both fungi in vitro. Assays involving inoculation of nonsterile soils with <i>B. bassiana</i> conidia showed trends toward elevated mortality of <i>L. decemlineata</i> in highly fungistatic soils compared to low-fungistasis ones. Introduction of antagonistic bacilli into sterile soil did not significantly change infectivity of <i>B. bassiana</i> toward the insect. The results support the idea that entomopathogenic fungi can infect insects within a hypogean habitat despite high abundance and diversity of soil antagonistic bacteria. |
| first_indexed | 2024-03-11T04:43:14Z |
| format | Article |
| id | doaj.art-1722a09b06b940d8819ba0132401c867 |
| institution | Directory Open Access Journal |
| issn | 2076-2607 |
| language | English |
| last_indexed | 2024-03-11T04:43:14Z |
| publishDate | 2023-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Microorganisms |
| spelling | doaj.art-1722a09b06b940d8819ba0132401c8672023-11-17T20:32:45ZengMDPI AGMicroorganisms2076-26072023-04-0111494310.3390/microorganisms11040943Links between Soil Bacteriobiomes and Fungistasis toward Fungi Infecting the Colorado Potato BeetleEkaterina Chertkova0Marsel R. Kabilov1Olga Yaroslavtseva2Olga Polenogova3Elena Kosman4Darya Sidorenko5Tatyana Alikina6Yury Noskov7Anton Krivopalov8Viktor V. Glupov9Vadim Yu. Kryukov10Institute of Systematics and Ecology of Animals, Siberian Branch of Russian Academy of Sciences, Novosibirsk 630091, RussiaInstitute of Chemical Biology and Fundamental Medicine, Siberian Branch of Russian Academy of Sciences, Novosibirsk 630090, RussiaInstitute of Systematics and Ecology of Animals, Siberian Branch of Russian Academy of Sciences, Novosibirsk 630091, RussiaInstitute of Systematics and Ecology of Animals, Siberian Branch of Russian Academy of Sciences, Novosibirsk 630091, RussiaInstitute of Systematics and Ecology of Animals, Siberian Branch of Russian Academy of Sciences, Novosibirsk 630091, RussiaInstitute of Systematics and Ecology of Animals, Siberian Branch of Russian Academy of Sciences, Novosibirsk 630091, RussiaInstitute of Chemical Biology and Fundamental Medicine, Siberian Branch of Russian Academy of Sciences, Novosibirsk 630090, RussiaInstitute of Systematics and Ecology of Animals, Siberian Branch of Russian Academy of Sciences, Novosibirsk 630091, RussiaInstitute of Systematics and Ecology of Animals, Siberian Branch of Russian Academy of Sciences, Novosibirsk 630091, RussiaInstitute of Systematics and Ecology of Animals, Siberian Branch of Russian Academy of Sciences, Novosibirsk 630091, RussiaInstitute of Systematics and Ecology of Animals, Siberian Branch of Russian Academy of Sciences, Novosibirsk 630091, RussiaEntomopathogenic fungi can be inhibited by different soil microorganisms, but the effect of a soil microbiota on fungal growth, survival, and infectivity toward insects is insufficiently understood. We investigated the level of fungistasis toward <i>Metarhizium robertsii</i> and <i>Beauveria bassiana</i> in soils of conventional potato fields and kitchen potato gardens. Agar diffusion methods, 16S rDNA metabarcoding, bacterial DNA quantification, and assays of <i>Leptinotarsa decemlineata</i> survival in soils inoculated with fungal conidia were used. Soils of kitchen gardens showed stronger fungistasis toward <i>M. robertsii</i> and <i>B. bassiana</i> and at the same time the highest density of the fungi compared to soils of conventional fields. The fungistasis level depended on the quantity of bacterial DNA and relative abundance of <i>Bacillus</i>, <i>Streptomyces</i>, and some <i>Proteobacteria</i>, whose abundance levels were the highest in kitchen garden soils. Cultivable isolates of bacilli exhibited antagonism to both fungi in vitro. Assays involving inoculation of nonsterile soils with <i>B. bassiana</i> conidia showed trends toward elevated mortality of <i>L. decemlineata</i> in highly fungistatic soils compared to low-fungistasis ones. Introduction of antagonistic bacilli into sterile soil did not significantly change infectivity of <i>B. bassiana</i> toward the insect. The results support the idea that entomopathogenic fungi can infect insects within a hypogean habitat despite high abundance and diversity of soil antagonistic bacteria.https://www.mdpi.com/2076-2607/11/4/943soil microbiotafungistasisentomopathogenic fungus<i>Beauveria</i><i>Metarhizium</i><i>Leptinotarsa decemlineata</i> |
| spellingShingle | Ekaterina Chertkova Marsel R. Kabilov Olga Yaroslavtseva Olga Polenogova Elena Kosman Darya Sidorenko Tatyana Alikina Yury Noskov Anton Krivopalov Viktor V. Glupov Vadim Yu. Kryukov Links between Soil Bacteriobiomes and Fungistasis toward Fungi Infecting the Colorado Potato Beetle Microorganisms soil microbiota fungistasis entomopathogenic fungus <i>Beauveria</i> <i>Metarhizium</i> <i>Leptinotarsa decemlineata</i> |
| title | Links between Soil Bacteriobiomes and Fungistasis toward Fungi Infecting the Colorado Potato Beetle |
| title_full | Links between Soil Bacteriobiomes and Fungistasis toward Fungi Infecting the Colorado Potato Beetle |
| title_fullStr | Links between Soil Bacteriobiomes and Fungistasis toward Fungi Infecting the Colorado Potato Beetle |
| title_full_unstemmed | Links between Soil Bacteriobiomes and Fungistasis toward Fungi Infecting the Colorado Potato Beetle |
| title_short | Links between Soil Bacteriobiomes and Fungistasis toward Fungi Infecting the Colorado Potato Beetle |
| title_sort | links between soil bacteriobiomes and fungistasis toward fungi infecting the colorado potato beetle |
| topic | soil microbiota fungistasis entomopathogenic fungus <i>Beauveria</i> <i>Metarhizium</i> <i>Leptinotarsa decemlineata</i> |
| url | https://www.mdpi.com/2076-2607/11/4/943 |
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