Myristic Acid Inhibits the Activity of the Bacterial ABC Transporter BmrA
ATP-binding cassette (ABC) transporters are conserved in all kingdoms of life, where they transport substrates against a concentration gradient across membranes. Some ABC transporters are known to cause multidrug resistances in humans and are able to transport chemotherapeutics across cellular membr...
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| Format: | Article |
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MDPI AG
2021-12-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/22/24/13565 |
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| author | Kristin Oepen Hüseyin Özbek Anja Schüffler Johannes C. Liermann Eckhard Thines Dirk Schneider |
| author_facet | Kristin Oepen Hüseyin Özbek Anja Schüffler Johannes C. Liermann Eckhard Thines Dirk Schneider |
| author_sort | Kristin Oepen |
| collection | DOAJ |
| description | ATP-binding cassette (ABC) transporters are conserved in all kingdoms of life, where they transport substrates against a concentration gradient across membranes. Some ABC transporters are known to cause multidrug resistances in humans and are able to transport chemotherapeutics across cellular membranes. Similarly, BmrA, the ABC transporter of <i>Bacillus subtilis,</i> is involved in excretion of certain antibiotics out of bacterial cells. Screening of extract libraries isolated from fungi revealed that the C14 fatty acid myristic acid has an inhibitory effect on the BmrA ATPase as well as the transport activity. Thus, a natural membrane constituent inhibits the BmrA activity, a finding with physiological consequences as to the activity and regulation of ABC transporter activities in biological membranes. |
| first_indexed | 2024-03-10T03:54:20Z |
| format | Article |
| id | doaj.art-1723998c87354982987cb686a9fc47ac |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T03:54:20Z |
| publishDate | 2021-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-1723998c87354982987cb686a9fc47ac2023-11-23T08:48:14ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672021-12-0122241356510.3390/ijms222413565Myristic Acid Inhibits the Activity of the Bacterial ABC Transporter BmrAKristin Oepen0Hüseyin Özbek1Anja Schüffler2Johannes C. Liermann3Eckhard Thines4Dirk Schneider5Department of Chemistry, Johannes Gutenberg-University, 55128 Mainz, GermanyInstitut für Biotechnologie und Wirkstoff-Forschung gGmbH (IBWF), 55128 Mainz, GermanyInstitut für Biotechnologie und Wirkstoff-Forschung gGmbH (IBWF), 55128 Mainz, GermanyDepartment of Chemistry, Johannes Gutenberg-University, 55128 Mainz, GermanyInstitut für Biotechnologie und Wirkstoff-Forschung gGmbH (IBWF), 55128 Mainz, GermanyDepartment of Chemistry, Johannes Gutenberg-University, 55128 Mainz, GermanyATP-binding cassette (ABC) transporters are conserved in all kingdoms of life, where they transport substrates against a concentration gradient across membranes. Some ABC transporters are known to cause multidrug resistances in humans and are able to transport chemotherapeutics across cellular membranes. Similarly, BmrA, the ABC transporter of <i>Bacillus subtilis,</i> is involved in excretion of certain antibiotics out of bacterial cells. Screening of extract libraries isolated from fungi revealed that the C14 fatty acid myristic acid has an inhibitory effect on the BmrA ATPase as well as the transport activity. Thus, a natural membrane constituent inhibits the BmrA activity, a finding with physiological consequences as to the activity and regulation of ABC transporter activities in biological membranes.https://www.mdpi.com/1422-0067/22/24/13565ABC transporterBmrAmembrane transportmyristic acidinhibitor |
| spellingShingle | Kristin Oepen Hüseyin Özbek Anja Schüffler Johannes C. Liermann Eckhard Thines Dirk Schneider Myristic Acid Inhibits the Activity of the Bacterial ABC Transporter BmrA International Journal of Molecular Sciences ABC transporter BmrA membrane transport myristic acid inhibitor |
| title | Myristic Acid Inhibits the Activity of the Bacterial ABC Transporter BmrA |
| title_full | Myristic Acid Inhibits the Activity of the Bacterial ABC Transporter BmrA |
| title_fullStr | Myristic Acid Inhibits the Activity of the Bacterial ABC Transporter BmrA |
| title_full_unstemmed | Myristic Acid Inhibits the Activity of the Bacterial ABC Transporter BmrA |
| title_short | Myristic Acid Inhibits the Activity of the Bacterial ABC Transporter BmrA |
| title_sort | myristic acid inhibits the activity of the bacterial abc transporter bmra |
| topic | ABC transporter BmrA membrane transport myristic acid inhibitor |
| url | https://www.mdpi.com/1422-0067/22/24/13565 |
| work_keys_str_mv | AT kristinoepen myristicacidinhibitstheactivityofthebacterialabctransporterbmra AT huseyinozbek myristicacidinhibitstheactivityofthebacterialabctransporterbmra AT anjaschuffler myristicacidinhibitstheactivityofthebacterialabctransporterbmra AT johannescliermann myristicacidinhibitstheactivityofthebacterialabctransporterbmra AT eckhardthines myristicacidinhibitstheactivityofthebacterialabctransporterbmra AT dirkschneider myristicacidinhibitstheactivityofthebacterialabctransporterbmra |