Genomic Surveillance of Listeria monocytogenes in Taiwan, 2014 to 2019
ABSTRACT Listeria monocytogenes is a life-threatening foodborne pathogen. Here, we report the genomic characterization of a nationwide dataset of 411 clinical and 82 food isolates collected in Taiwan between 2014 and 2019. The observed incidence of listeriosis increased from 0.83 to 7 cases per mill...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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American Society for Microbiology
2022-12-01
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| Series: | Microbiology Spectrum |
| Subjects: | |
| Online Access: | https://journals.asm.org/doi/10.1128/spectrum.01825-22 |
| _version_ | 1811290124035555328 |
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| author | Yu-Huan Tsai Alexandra Moura Zi-Qi Gu Jui-Hsien Chang Ying-Shu Liao Ru-Hsiou Teng Kuo-Yao Tseng Dai-Ling Chang Wei-Ren Liu Yu-Tsung Huang Alexandre Leclercq Hsiu-Jung Lo Marc Lecuit Chien-Shun Chiou |
| author_facet | Yu-Huan Tsai Alexandra Moura Zi-Qi Gu Jui-Hsien Chang Ying-Shu Liao Ru-Hsiou Teng Kuo-Yao Tseng Dai-Ling Chang Wei-Ren Liu Yu-Tsung Huang Alexandre Leclercq Hsiu-Jung Lo Marc Lecuit Chien-Shun Chiou |
| author_sort | Yu-Huan Tsai |
| collection | DOAJ |
| description | ABSTRACT Listeria monocytogenes is a life-threatening foodborne pathogen. Here, we report the genomic characterization of a nationwide dataset of 411 clinical and 82 food isolates collected in Taiwan between 2014 and 2019. The observed incidence of listeriosis increased from 0.83 to 7 cases per million population upon implementation of mandatory notification in 2018. Pregnancy-associated cases accounted for 2.8% of human listeriosis and all-cause 7-day mortality was of 11.9% in nonmaternal-neonatal listeriosis. L. monocytogenes was isolated from 90% of raw pork and 34% of chicken products collected in supermarkets. Sublineages SL87, SL5, and SL378 accounted for the majority (65%) of clinical cases. SL87 and SL378 were also predominant (57%) in food products. Five cgMLST clusters accounted for 57% clinical cases, suggesting unnoticed outbreaks spanning up to 6 years. Mandatory notification allowed identifying the magnitude of listeriosis in Taiwan. Continuous real-time genomic surveillance will allow reducing contaminating sources and disease burden. IMPORTANCE Understanding the phylogenetic relationship between clinical and food isolates is important to identify the transmission routes of foodborne diseases. Here, we performed a nationwide study between 2014 and 2019 of both clinical and food Listeria monocytogenes isolates and sequenced their genomes. We show a 9-fold increase in listeriosis reporting upon implementation of mandatory notification. We found that sublineages SL87 and SL378 predominated among both clinical (50%) and food (57%) isolates, and identified five cgMLST clusters accounting for 57% of clinical cases, suggestive of potential protracted sources of contamination over up to 6 years in Taiwan. These findings highlight that mandatory declaration is critical in identifying the burden of listeriosis, and the importance of genome sequencing for a detailed characterization of the pathogenic L. monocytogenes genotypes circulating in Asia. |
| first_indexed | 2024-04-13T04:07:24Z |
| format | Article |
| id | doaj.art-172798d974a544b4b3d908e89d640b70 |
| institution | Directory Open Access Journal |
| issn | 2165-0497 |
| language | English |
| last_indexed | 2024-04-13T04:07:24Z |
| publishDate | 2022-12-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | Microbiology Spectrum |
| spelling | doaj.art-172798d974a544b4b3d908e89d640b702022-12-22T03:03:12ZengAmerican Society for MicrobiologyMicrobiology Spectrum2165-04972022-12-0110610.1128/spectrum.01825-22Genomic Surveillance of Listeria monocytogenes in Taiwan, 2014 to 2019Yu-Huan Tsai0Alexandra Moura1Zi-Qi Gu2Jui-Hsien Chang3Ying-Shu Liao4Ru-Hsiou Teng5Kuo-Yao Tseng6Dai-Ling Chang7Wei-Ren Liu8Yu-Tsung Huang9Alexandre Leclercq10Hsiu-Jung Lo11Marc Lecuit12Chien-Shun Chiou13Laboratory of Host-Microbe Interactions and Cell Dynamics, Institute of Microbiology and Immunology, National Yang Ming Chiao Tung University, Taipei, TaiwanBiology of Infection Unit, Institut Pasteur, Université Paris Cité, Inserm U1117, Paris, FranceLaboratory of Host-Microbe Interactions and Cell Dynamics, Institute of Microbiology and Immunology, National Yang Ming Chiao Tung University, Taipei, TaiwanCenter for Diagnostics and Vaccine Development, Centers for Disease Control, Taichung, TaiwanCenter for Diagnostics and Vaccine Development, Centers for Disease Control, Taichung, TaiwanCenter for Diagnostics and Vaccine Development, Centers for Disease Control, Taichung, TaiwanLaboratory of Host-Microbe Interactions and Cell Dynamics, Institute of Microbiology and Immunology, National Yang Ming Chiao Tung University, Taipei, TaiwanLaboratory of Host-Microbe Interactions and Cell Dynamics, Institute of Microbiology and Immunology, National Yang Ming Chiao Tung University, Taipei, TaiwanLaboratory of Host-Microbe Interactions and Cell Dynamics, Institute of Microbiology and Immunology, National Yang Ming Chiao Tung University, Taipei, TaiwanDepartments of Laboratory Medicine and Internal Medicine, National Taiwan University Hospital, National Taiwan University College of Medicine, Taipei, TaiwanBiology of Infection Unit, Institut Pasteur, Université Paris Cité, Inserm U1117, Paris, FranceNational Institute of Infectious Disease and Vaccinology, National Health Research Institutes, Miaoli, TaiwanBiology of Infection Unit, Institut Pasteur, Université Paris Cité, Inserm U1117, Paris, FranceCenter for Diagnostics and Vaccine Development, Centers for Disease Control, Taichung, TaiwanABSTRACT Listeria monocytogenes is a life-threatening foodborne pathogen. Here, we report the genomic characterization of a nationwide dataset of 411 clinical and 82 food isolates collected in Taiwan between 2014 and 2019. The observed incidence of listeriosis increased from 0.83 to 7 cases per million population upon implementation of mandatory notification in 2018. Pregnancy-associated cases accounted for 2.8% of human listeriosis and all-cause 7-day mortality was of 11.9% in nonmaternal-neonatal listeriosis. L. monocytogenes was isolated from 90% of raw pork and 34% of chicken products collected in supermarkets. Sublineages SL87, SL5, and SL378 accounted for the majority (65%) of clinical cases. SL87 and SL378 were also predominant (57%) in food products. Five cgMLST clusters accounted for 57% clinical cases, suggesting unnoticed outbreaks spanning up to 6 years. Mandatory notification allowed identifying the magnitude of listeriosis in Taiwan. Continuous real-time genomic surveillance will allow reducing contaminating sources and disease burden. IMPORTANCE Understanding the phylogenetic relationship between clinical and food isolates is important to identify the transmission routes of foodborne diseases. Here, we performed a nationwide study between 2014 and 2019 of both clinical and food Listeria monocytogenes isolates and sequenced their genomes. We show a 9-fold increase in listeriosis reporting upon implementation of mandatory notification. We found that sublineages SL87 and SL378 predominated among both clinical (50%) and food (57%) isolates, and identified five cgMLST clusters accounting for 57% of clinical cases, suggestive of potential protracted sources of contamination over up to 6 years in Taiwan. These findings highlight that mandatory declaration is critical in identifying the burden of listeriosis, and the importance of genome sequencing for a detailed characterization of the pathogenic L. monocytogenes genotypes circulating in Asia.https://journals.asm.org/doi/10.1128/spectrum.01825-22core genome multilocus sequence typingListeria monocytogeneslisteriosiswhole-genome sequencing |
| spellingShingle | Yu-Huan Tsai Alexandra Moura Zi-Qi Gu Jui-Hsien Chang Ying-Shu Liao Ru-Hsiou Teng Kuo-Yao Tseng Dai-Ling Chang Wei-Ren Liu Yu-Tsung Huang Alexandre Leclercq Hsiu-Jung Lo Marc Lecuit Chien-Shun Chiou Genomic Surveillance of Listeria monocytogenes in Taiwan, 2014 to 2019 Microbiology Spectrum core genome multilocus sequence typing Listeria monocytogenes listeriosis whole-genome sequencing |
| title | Genomic Surveillance of Listeria monocytogenes in Taiwan, 2014 to 2019 |
| title_full | Genomic Surveillance of Listeria monocytogenes in Taiwan, 2014 to 2019 |
| title_fullStr | Genomic Surveillance of Listeria monocytogenes in Taiwan, 2014 to 2019 |
| title_full_unstemmed | Genomic Surveillance of Listeria monocytogenes in Taiwan, 2014 to 2019 |
| title_short | Genomic Surveillance of Listeria monocytogenes in Taiwan, 2014 to 2019 |
| title_sort | genomic surveillance of listeria monocytogenes in taiwan 2014 to 2019 |
| topic | core genome multilocus sequence typing Listeria monocytogenes listeriosis whole-genome sequencing |
| url | https://journals.asm.org/doi/10.1128/spectrum.01825-22 |
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