Therapeutic effect of total flavonoids of Sargentodoxa cuneata on ulcerative colitis in mice by correcting gut dysbiosis

Sargentodoxa cuneata is a traditional Chinese herb commonly used for the treatment of ulcerative colitis (UC) in clinical practice. However, the effects of total flavonoids of Sargentodoxa cuneata (TFSc) remain unclear. In this study, we explored the effects and mechanisms of action of TFSc in mice...

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Main Authors: Feng Xu, Piao Yu, Hongmei Wu, Xiangpei Wang, Mei Liu, Hongyun Liu, Qian Zeng, Dengli Wu
Format: Article
Language:English
Published: Elsevier 2024-02-01
Series:Arabian Journal of Chemistry
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S1878535223010286
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author Feng Xu
Piao Yu
Hongmei Wu
Xiangpei Wang
Mei Liu
Hongyun Liu
Qian Zeng
Dengli Wu
author_facet Feng Xu
Piao Yu
Hongmei Wu
Xiangpei Wang
Mei Liu
Hongyun Liu
Qian Zeng
Dengli Wu
author_sort Feng Xu
collection DOAJ
description Sargentodoxa cuneata is a traditional Chinese herb commonly used for the treatment of ulcerative colitis (UC) in clinical practice. However, the effects of total flavonoids of Sargentodoxa cuneata (TFSc) remain unclear. In this study, we explored the effects and mechanisms of action of TFSc in mice with dextran sulfate sodium-induced UC. The TFSc components were identified using liquid chromatography-tandem mass spectrometry. Mice with UC were orally administered TFSc to evaluate its effects on colon and liver damage. Changes in gut microbiota were analyzed using 16S rRNA gene sequencing. Moreover, the levels of short-chain fatty acids (SCFAs) and few organic acids in the intestinal contents were measured. Furthermore, the correlation amongst gut flora, SCFAs, and organic acids was studied. A total of 304 compounds were identified. TFSc alleviated the symptoms of UC in mice, inhibited weight loss, reduced the disease activity index, and improved colon shortening along with cardiac and hepatic indices. Furthermore, TFSc mitigated pathological changes in the colon and liver, and reduced the TNF-α levels in colon tissues of mice with UC. TFSc also modulated intestinal flora disorders, increased the levels of four SCFAs (isocaproic acid, 3-hydroxyisovaleric acid, isobutyric acid, and ethylmethylacetic acid), and decreased the levels of two organic acids (malonic acid and succinic acid). The differential regulation of SCFAs and organic acids by TFSc was strongly associated with specific gut flora. TFSc showed potential for improving colon and liver injury in mice with UC by regulating the intestinal flora and associated metabolites (SCFAs and organic acids). These findings provide new evidence for developing TFSc as a drug for treating UC and UC-related liver injuries.
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spelling doaj.art-1727c2d70dd245bc8fa405aafe74ab062024-01-14T05:37:58ZengElsevierArabian Journal of Chemistry1878-53522024-02-01172105566Therapeutic effect of total flavonoids of Sargentodoxa cuneata on ulcerative colitis in mice by correcting gut dysbiosisFeng Xu0Piao Yu1Hongmei Wu2Xiangpei Wang3Mei Liu4Hongyun Liu5Qian Zeng6Dengli Wu7Guizhou University of Traditional Chinese Medicine, Department of Pharmacy, Guiyang, China; Corresponding author at: College of Pharmacy Guizhou University of Traditional Chinese Medicine, Huaxi District, Guiyang City, Guizhou Province, Guiyang 550025, China.Guizhou University of Traditional Chinese Medicine, Department of Pharmacy, Guiyang, ChinaGuizhou University of Traditional Chinese Medicine, Department of Pharmacy, Guiyang, ChinaGuizhou Minzu University, School of Chinese Ethnic Medicine, Guiyang, ChinaGuizhou University of Traditional Chinese Medicine, Department of Pharmacy, Guiyang, ChinaGuizhou University of Traditional Chinese Medicine, Department of Pharmacy, Guiyang, ChinaGuizhou University of Traditional Chinese Medicine, Department of Pharmacy, Guiyang, ChinaGuizhou University of Traditional Chinese Medicine, Department of Pharmacy, Guiyang, ChinaSargentodoxa cuneata is a traditional Chinese herb commonly used for the treatment of ulcerative colitis (UC) in clinical practice. However, the effects of total flavonoids of Sargentodoxa cuneata (TFSc) remain unclear. In this study, we explored the effects and mechanisms of action of TFSc in mice with dextran sulfate sodium-induced UC. The TFSc components were identified using liquid chromatography-tandem mass spectrometry. Mice with UC were orally administered TFSc to evaluate its effects on colon and liver damage. Changes in gut microbiota were analyzed using 16S rRNA gene sequencing. Moreover, the levels of short-chain fatty acids (SCFAs) and few organic acids in the intestinal contents were measured. Furthermore, the correlation amongst gut flora, SCFAs, and organic acids was studied. A total of 304 compounds were identified. TFSc alleviated the symptoms of UC in mice, inhibited weight loss, reduced the disease activity index, and improved colon shortening along with cardiac and hepatic indices. Furthermore, TFSc mitigated pathological changes in the colon and liver, and reduced the TNF-α levels in colon tissues of mice with UC. TFSc also modulated intestinal flora disorders, increased the levels of four SCFAs (isocaproic acid, 3-hydroxyisovaleric acid, isobutyric acid, and ethylmethylacetic acid), and decreased the levels of two organic acids (malonic acid and succinic acid). The differential regulation of SCFAs and organic acids by TFSc was strongly associated with specific gut flora. TFSc showed potential for improving colon and liver injury in mice with UC by regulating the intestinal flora and associated metabolites (SCFAs and organic acids). These findings provide new evidence for developing TFSc as a drug for treating UC and UC-related liver injuries.http://www.sciencedirect.com/science/article/pii/S1878535223010286Total flavonoids of Sargentodoxa cuneataUlcerative colitisUlcerative colitis-related liver injuryGut microbiotaShort-chain fatty acidsOrganic acids
spellingShingle Feng Xu
Piao Yu
Hongmei Wu
Xiangpei Wang
Mei Liu
Hongyun Liu
Qian Zeng
Dengli Wu
Therapeutic effect of total flavonoids of Sargentodoxa cuneata on ulcerative colitis in mice by correcting gut dysbiosis
Arabian Journal of Chemistry
Total flavonoids of Sargentodoxa cuneata
Ulcerative colitis
Ulcerative colitis-related liver injury
Gut microbiota
Short-chain fatty acids
Organic acids
title Therapeutic effect of total flavonoids of Sargentodoxa cuneata on ulcerative colitis in mice by correcting gut dysbiosis
title_full Therapeutic effect of total flavonoids of Sargentodoxa cuneata on ulcerative colitis in mice by correcting gut dysbiosis
title_fullStr Therapeutic effect of total flavonoids of Sargentodoxa cuneata on ulcerative colitis in mice by correcting gut dysbiosis
title_full_unstemmed Therapeutic effect of total flavonoids of Sargentodoxa cuneata on ulcerative colitis in mice by correcting gut dysbiosis
title_short Therapeutic effect of total flavonoids of Sargentodoxa cuneata on ulcerative colitis in mice by correcting gut dysbiosis
title_sort therapeutic effect of total flavonoids of sargentodoxa cuneata on ulcerative colitis in mice by correcting gut dysbiosis
topic Total flavonoids of Sargentodoxa cuneata
Ulcerative colitis
Ulcerative colitis-related liver injury
Gut microbiota
Short-chain fatty acids
Organic acids
url http://www.sciencedirect.com/science/article/pii/S1878535223010286
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