TRAF3 gene regulates macrophage migration and activation by lung epithelial cells infected with Aspergillus fumigatus
ABSTRACT Aspergillus fumigatus is an opportunistic pathogen that infects immunocompromised patients and imposes a heavy burden on global health. Searching for key genes in host resistance to A. fumigatus infection can help us understand the mechanisms of A. fumigatus-host interactions and provide ne...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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American Society for Microbiology
2024-01-01
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| Series: | Microbiology Spectrum |
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| Online Access: | https://journals.asm.org/doi/10.1128/spectrum.02699-23 |
| _version_ | 1797357407438897152 |
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| author | Shumi Shang Dan He Cong Liu Xinyuan Bao Shuaishuai Han Li Wang |
| author_facet | Shumi Shang Dan He Cong Liu Xinyuan Bao Shuaishuai Han Li Wang |
| author_sort | Shumi Shang |
| collection | DOAJ |
| description | ABSTRACT Aspergillus fumigatus is an opportunistic pathogen that infects immunocompromised patients and imposes a heavy burden on global health. Searching for key genes in host resistance to A. fumigatus infection can help us understand the mechanisms of A. fumigatus-host interactions and provide new targets for the treatment of fungal infections. TRAF3 is one of the intracellular adapter proteins of the innate immune response that regulates signaling in various cellular processes, including host defense against pathogens. However, the defense mechanism of TRAF3 against A. fumigatus infection remains unknown. In this study, we found that TRAF3 overexpression led to the adhesion and internalization of more spores of A. fumigatus in lung epithelial cells and thus greater host immune surveillance evasion. Meanwhile, TRAF3 was able to regulate the expression of pro-inflammatory cytokines in lung epithelial cells infected with A. fumigatus through the negative regulation of NF-κB and MAPK signaling pathways. In this study, we also examined the effects of TRAF3 in the interaction between lung epithelial cells, macrophages, and A. fumigatus spores, and the results showed that TRAF3-overexpressing lung epithelial cells reduced the migration and activation of macrophages after A. fumigatus infection. In vivo experiments using TRAF3-overexpressing transgenic zebrafish larvae revealed that TRAF3 overexpression increased the fungal load and mortality of zebrafish infected with A. fumigatus. In conclusion, the TRAF3 gene can negatively regulate the resistance of lung epithelial cells to A. fumigatus, which plays an important role in the early infection processes of A. fumigatus. IMPORTANCE Aspergillus fumigatus can infect immunocompromised individuals and cause chronic and fatal invasive fungal infections. A better understanding of the molecular mechanisms of A. fumigatus-host interactions may provide new references for disease treatment. In this study, we demonstrated that the TRAF3 gene plays an important role in the early infection of A. fumigatus by regulating the resistance of lung epithelial cells to A. fumigatus. Macrophages are the most abundant innate immune cells in the alveoli; however, few studies have reported on the interactions between lung epithelial cells and macrophages in response to A. fumigatus invasion. In our study, it was demonstrated that the TRAF3 gene reduces migration to macrophages and cytokine production by negatively regulating lung epithelial cell adhesion and internalization of A. fumigatus spores. Together, our results provide new insights into lung epithelial cell-macrophage interactions during A. fumigatus infection. |
| first_indexed | 2024-03-08T14:43:35Z |
| format | Article |
| id | doaj.art-172df040d5a04cb793b67b8e20b2f86a |
| institution | Directory Open Access Journal |
| issn | 2165-0497 |
| language | English |
| last_indexed | 2024-03-08T14:43:35Z |
| publishDate | 2024-01-01 |
| publisher | American Society for Microbiology |
| record_format | Article |
| series | Microbiology Spectrum |
| spelling | doaj.art-172df040d5a04cb793b67b8e20b2f86a2024-01-11T14:04:37ZengAmerican Society for MicrobiologyMicrobiology Spectrum2165-04972024-01-0112110.1128/spectrum.02699-23TRAF3 gene regulates macrophage migration and activation by lung epithelial cells infected with Aspergillus fumigatusShumi Shang0Dan He1Cong Liu2Xinyuan Bao3Shuaishuai Han4Li Wang5Department of Pathogenobiology, Jilin University Mycology Research Center, Key Laboratory of Zoonosis Research, Ministry of Education, College of Basic Medical Sciences, Jilin University , Changchun, ChinaDepartment of Pathogenobiology, Jilin University Mycology Research Center, Key Laboratory of Zoonosis Research, Ministry of Education, College of Basic Medical Sciences, Jilin University , Changchun, ChinaDepartment of Dermatology, First Affiliated Hospital of Dalian Medical University , Dalian, ChinaDepartment of Pathogenobiology, Jilin University Mycology Research Center, Key Laboratory of Zoonosis Research, Ministry of Education, College of Basic Medical Sciences, Jilin University , Changchun, ChinaBeijing ZhongKai TianCheng Bio-technology Co. Ltd. , Beijing, ChinaDepartment of Pathogenobiology, Jilin University Mycology Research Center, Key Laboratory of Zoonosis Research, Ministry of Education, College of Basic Medical Sciences, Jilin University , Changchun, ChinaABSTRACT Aspergillus fumigatus is an opportunistic pathogen that infects immunocompromised patients and imposes a heavy burden on global health. Searching for key genes in host resistance to A. fumigatus infection can help us understand the mechanisms of A. fumigatus-host interactions and provide new targets for the treatment of fungal infections. TRAF3 is one of the intracellular adapter proteins of the innate immune response that regulates signaling in various cellular processes, including host defense against pathogens. However, the defense mechanism of TRAF3 against A. fumigatus infection remains unknown. In this study, we found that TRAF3 overexpression led to the adhesion and internalization of more spores of A. fumigatus in lung epithelial cells and thus greater host immune surveillance evasion. Meanwhile, TRAF3 was able to regulate the expression of pro-inflammatory cytokines in lung epithelial cells infected with A. fumigatus through the negative regulation of NF-κB and MAPK signaling pathways. In this study, we also examined the effects of TRAF3 in the interaction between lung epithelial cells, macrophages, and A. fumigatus spores, and the results showed that TRAF3-overexpressing lung epithelial cells reduced the migration and activation of macrophages after A. fumigatus infection. In vivo experiments using TRAF3-overexpressing transgenic zebrafish larvae revealed that TRAF3 overexpression increased the fungal load and mortality of zebrafish infected with A. fumigatus. In conclusion, the TRAF3 gene can negatively regulate the resistance of lung epithelial cells to A. fumigatus, which plays an important role in the early infection processes of A. fumigatus. IMPORTANCE Aspergillus fumigatus can infect immunocompromised individuals and cause chronic and fatal invasive fungal infections. A better understanding of the molecular mechanisms of A. fumigatus-host interactions may provide new references for disease treatment. In this study, we demonstrated that the TRAF3 gene plays an important role in the early infection of A. fumigatus by regulating the resistance of lung epithelial cells to A. fumigatus. Macrophages are the most abundant innate immune cells in the alveoli; however, few studies have reported on the interactions between lung epithelial cells and macrophages in response to A. fumigatus invasion. In our study, it was demonstrated that the TRAF3 gene reduces migration to macrophages and cytokine production by negatively regulating lung epithelial cell adhesion and internalization of A. fumigatus spores. Together, our results provide new insights into lung epithelial cell-macrophage interactions during A. fumigatus infection.https://journals.asm.org/doi/10.1128/spectrum.02699-23TRAF3Aspergillus fumigatuslung epithelial cellsmacrophagezebrafish |
| spellingShingle | Shumi Shang Dan He Cong Liu Xinyuan Bao Shuaishuai Han Li Wang TRAF3 gene regulates macrophage migration and activation by lung epithelial cells infected with Aspergillus fumigatus Microbiology Spectrum TRAF3 Aspergillus fumigatus lung epithelial cells macrophage zebrafish |
| title | TRAF3 gene regulates macrophage migration and activation by lung epithelial cells infected with Aspergillus fumigatus |
| title_full | TRAF3 gene regulates macrophage migration and activation by lung epithelial cells infected with Aspergillus fumigatus |
| title_fullStr | TRAF3 gene regulates macrophage migration and activation by lung epithelial cells infected with Aspergillus fumigatus |
| title_full_unstemmed | TRAF3 gene regulates macrophage migration and activation by lung epithelial cells infected with Aspergillus fumigatus |
| title_short | TRAF3 gene regulates macrophage migration and activation by lung epithelial cells infected with Aspergillus fumigatus |
| title_sort | traf3 gene regulates macrophage migration and activation by lung epithelial cells infected with aspergillus fumigatus |
| topic | TRAF3 Aspergillus fumigatus lung epithelial cells macrophage zebrafish |
| url | https://journals.asm.org/doi/10.1128/spectrum.02699-23 |
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