Integrated Metabolomic and Transcriptomic Analysis Reveals Differential Flavonoid Accumulation and Its Underlying Mechanism in Fruits of Distinct <i>Canarium album</i> Cultivars
<i>Canarium album</i> fruit has great potential to be consumed as a raw material not only for food but also medicine. The diverse active metabolites composition and content of <i>C. album</i> fruits greatly affect their pharmacological effects. However, up to now, there has b...
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| Format: | Article |
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MDPI AG
2022-08-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/11/16/2527 |
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| author | Ruilian Lai Chaogui Shen Xin Feng Minxia Gao Yongyan Zhang Xiaoxia Wei Yiting Chen Chunzhen Cheng Rujian Wu |
| author_facet | Ruilian Lai Chaogui Shen Xin Feng Minxia Gao Yongyan Zhang Xiaoxia Wei Yiting Chen Chunzhen Cheng Rujian Wu |
| author_sort | Ruilian Lai |
| collection | DOAJ |
| description | <i>Canarium album</i> fruit has great potential to be consumed as a raw material not only for food but also medicine. The diverse active metabolites composition and content of <i>C. album</i> fruits greatly affect their pharmacological effects. However, up to now, there has been no report on the global metabolome differences among fruits from distinct <i>C. album</i> cultivars. In our present study, by using non-targeted metabolomics techniques, we identified 87 DAMs (differentially accumulated metabolites) including 17 types of flavonoids from fruits of four different <i>C. album</i> cultivars. KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway enrichment analysis revealed that the flavone and flavonol biosynthesis- and flavonoid biosynthesis-related DAMs were major factors determining their metabolome differences. Comparative transcriptomic analysis revealed that 15 KEGG pathways were significantly enriched by genes of the identified 3655 DEGs (differentially expressed genes) among different <i>C. album</i> cultivars. Consistent with the metabolome data, flavonoid biosynthesis-related DEGs, including eight key structural genes (such as <i>FLS</i>, <i>CCoAOMT</i>, <i>CHI</i>, <i>C4H</i>, <i>DFR</i>, <i>LAR</i>, and <i>C3′H</i>, etc.) and several regulatory transcription factor (TF) genes (including 32 <i>MYBs</i> and 34 <i>bHLHs</i>, etc.), were found to be significantly enriched (<i>p</i> < 0.01). Our study indicated that the differential expression of flavonoid biosynthesis-related genes and accumulation of flavonoids played dominant roles in the various metabolome compositions of fruits from different <i>C. album</i> cultivars. |
| first_indexed | 2024-03-09T13:25:59Z |
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| institution | Directory Open Access Journal |
| issn | 2304-8158 |
| language | English |
| last_indexed | 2024-03-09T13:25:59Z |
| publishDate | 2022-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Foods |
| spelling | doaj.art-172e3b794a4a4c33b714e6fc34266a562023-11-30T21:23:59ZengMDPI AGFoods2304-81582022-08-011116252710.3390/foods11162527Integrated Metabolomic and Transcriptomic Analysis Reveals Differential Flavonoid Accumulation and Its Underlying Mechanism in Fruits of Distinct <i>Canarium album</i> CultivarsRuilian Lai0Chaogui Shen1Xin Feng2Minxia Gao3Yongyan Zhang4Xiaoxia Wei5Yiting Chen6Chunzhen Cheng7Rujian Wu8Fruit Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350013, ChinaFruit Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350013, ChinaFruit Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350013, ChinaFruit Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350013, ChinaCollege of Horticulture, Shanxi Agricultural University, Jinzhong 030801, ChinaFruit Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350013, ChinaFruit Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350013, ChinaCollege of Horticulture, Shanxi Agricultural University, Jinzhong 030801, ChinaFruit Research Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350013, China<i>Canarium album</i> fruit has great potential to be consumed as a raw material not only for food but also medicine. The diverse active metabolites composition and content of <i>C. album</i> fruits greatly affect their pharmacological effects. However, up to now, there has been no report on the global metabolome differences among fruits from distinct <i>C. album</i> cultivars. In our present study, by using non-targeted metabolomics techniques, we identified 87 DAMs (differentially accumulated metabolites) including 17 types of flavonoids from fruits of four different <i>C. album</i> cultivars. KEGG (Kyoto Encyclopedia of Genes and Genomes) pathway enrichment analysis revealed that the flavone and flavonol biosynthesis- and flavonoid biosynthesis-related DAMs were major factors determining their metabolome differences. Comparative transcriptomic analysis revealed that 15 KEGG pathways were significantly enriched by genes of the identified 3655 DEGs (differentially expressed genes) among different <i>C. album</i> cultivars. Consistent with the metabolome data, flavonoid biosynthesis-related DEGs, including eight key structural genes (such as <i>FLS</i>, <i>CCoAOMT</i>, <i>CHI</i>, <i>C4H</i>, <i>DFR</i>, <i>LAR</i>, and <i>C3′H</i>, etc.) and several regulatory transcription factor (TF) genes (including 32 <i>MYBs</i> and 34 <i>bHLHs</i>, etc.), were found to be significantly enriched (<i>p</i> < 0.01). Our study indicated that the differential expression of flavonoid biosynthesis-related genes and accumulation of flavonoids played dominant roles in the various metabolome compositions of fruits from different <i>C. album</i> cultivars.https://www.mdpi.com/2304-8158/11/16/2527<i>Canarium album</i>flavonoidmetabolomicstranscriptomicsgene expression |
| spellingShingle | Ruilian Lai Chaogui Shen Xin Feng Minxia Gao Yongyan Zhang Xiaoxia Wei Yiting Chen Chunzhen Cheng Rujian Wu Integrated Metabolomic and Transcriptomic Analysis Reveals Differential Flavonoid Accumulation and Its Underlying Mechanism in Fruits of Distinct <i>Canarium album</i> Cultivars Foods <i>Canarium album</i> flavonoid metabolomics transcriptomics gene expression |
| title | Integrated Metabolomic and Transcriptomic Analysis Reveals Differential Flavonoid Accumulation and Its Underlying Mechanism in Fruits of Distinct <i>Canarium album</i> Cultivars |
| title_full | Integrated Metabolomic and Transcriptomic Analysis Reveals Differential Flavonoid Accumulation and Its Underlying Mechanism in Fruits of Distinct <i>Canarium album</i> Cultivars |
| title_fullStr | Integrated Metabolomic and Transcriptomic Analysis Reveals Differential Flavonoid Accumulation and Its Underlying Mechanism in Fruits of Distinct <i>Canarium album</i> Cultivars |
| title_full_unstemmed | Integrated Metabolomic and Transcriptomic Analysis Reveals Differential Flavonoid Accumulation and Its Underlying Mechanism in Fruits of Distinct <i>Canarium album</i> Cultivars |
| title_short | Integrated Metabolomic and Transcriptomic Analysis Reveals Differential Flavonoid Accumulation and Its Underlying Mechanism in Fruits of Distinct <i>Canarium album</i> Cultivars |
| title_sort | integrated metabolomic and transcriptomic analysis reveals differential flavonoid accumulation and its underlying mechanism in fruits of distinct i canarium album i cultivars |
| topic | <i>Canarium album</i> flavonoid metabolomics transcriptomics gene expression |
| url | https://www.mdpi.com/2304-8158/11/16/2527 |
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