Oral microbiota and periodontitis severity among Hispanic adults
BackgroundPeriodontitis, one of the most common bacterial infections characterized by chronic inflammation, is also known to be a risk factor for chronic conditions, including cardiovascular disease and cancer. This inflammation is driven by an altered microbiota with an increase in pathogenic bacte...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2022-11-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
| Subjects: | |
| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2022.965159/full |
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| author | Ana P. Ortiz Ana P. Ortiz Kimil T. Acosta-Pagán Carla Oramas-Sepúlveda Maira A. Castañeda-Avila Brayan Vilanova-Cuevas Jeslie M. Ramos-Cartagena José A. Vivaldi Josué Pérez-Santiago Cynthia M. Pérez Filipa Godoy-Vitorino |
| author_facet | Ana P. Ortiz Ana P. Ortiz Kimil T. Acosta-Pagán Carla Oramas-Sepúlveda Maira A. Castañeda-Avila Brayan Vilanova-Cuevas Jeslie M. Ramos-Cartagena José A. Vivaldi Josué Pérez-Santiago Cynthia M. Pérez Filipa Godoy-Vitorino |
| author_sort | Ana P. Ortiz |
| collection | DOAJ |
| description | BackgroundPeriodontitis, one of the most common bacterial infections characterized by chronic inflammation, is also known to be a risk factor for chronic conditions, including cardiovascular disease and cancer. This inflammation is driven by an altered microbiota with an increase in pathogenic bacteria. We evaluated the association between oral microbiota and periodontitis severity in high-risk Hispanics.MethodThis cross-sectional study recruited 134 sexually active participants aged 21 to 49 years old from STI Clinics in Puerto Rico. A periodontal examination, saliva collection, and an interviewer-administered questionnaire were performed. Periodontal severity was categorized as: having no disease, mild, and moderate/severe and BOP and tooth loos was noted. Saliva samples were collected for genomic DNA extraction, downstream 16S rDNA amplification sequencing, and bioinformatics analyses.ResultsThe structure, composition, and diversity of bacterial communities differed significantly according to periodontal severity. The richness and overall diversity also differed between participants without periodontitis and participants with some level of periodontal disease. A higher abundance of Prevotella, Veillonella, or Treponema was attributed to periodontal disease and Aggregatibacter to severe bleeding on probing, while Neisseria was found in higher abundance in healthy participants, decreasing its levels with drinking, smoking, and oral sex practices.ConclusionsOur findings indicate that dysbiosis occurs as periodontal disease progresses, and both alcohol consumption and smoking habits pose risk factors for oral dysbiosis. These results are of public health and clinical impact, as several bacteria identified could serve in the future as biomarkers for periodontitis and oral cancer risk. |
| first_indexed | 2024-04-11T06:46:32Z |
| format | Article |
| id | doaj.art-172ec6a033224253bca9be52090a8e1c |
| institution | Directory Open Access Journal |
| issn | 2235-2988 |
| language | English |
| last_indexed | 2024-04-11T06:46:32Z |
| publishDate | 2022-11-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj.art-172ec6a033224253bca9be52090a8e1c2022-12-22T04:39:19ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882022-11-011210.3389/fcimb.2022.965159965159Oral microbiota and periodontitis severity among Hispanic adultsAna P. Ortiz0Ana P. Ortiz1Kimil T. Acosta-Pagán2Carla Oramas-Sepúlveda3Maira A. Castañeda-Avila4Brayan Vilanova-Cuevas5Jeslie M. Ramos-Cartagena6José A. Vivaldi7Josué Pérez-Santiago8Cynthia M. Pérez9Filipa Godoy-Vitorino10Division of Cancer Control and Population Sciences, University of Puerto Rico Comprehensive Cancer Center, San Juan, Puerto RicoGraduate School of Public Health, Medical Sciences Campus, University of Puerto Rico, San Juan, Puerto RicoDepartment of Microbiology and Medical Zoology, Medical Sciences Campus, University of Puerto Rico, San Juan, Puerto RicoDivision of Cancer Control and Population Sciences, University of Puerto Rico Comprehensive Cancer Center, San Juan, Puerto RicoUniversity of Massachusetts Chan Medical School, Population and Quantitative Health Science, Worcester, MA, United StatesDepartment of Microbiology and Medical Zoology, Medical Sciences Campus, University of Puerto Rico, San Juan, Puerto RicoUniversity of Puerto Rico, Medical Sciences Campus, UPRMDACC Partnership for Excellence in Cancer Research Program, San Juan, Puerto RicoSchool of Dental Medicine, Recinto Gurabo, Universidad Ana G. Mendez, Gurabo, Puerto RicoDivision of Cancer Control and Population Sciences, University of Puerto Rico Comprehensive Cancer Center, San Juan, Puerto RicoGraduate School of Public Health, Medical Sciences Campus, University of Puerto Rico, San Juan, Puerto RicoDepartment of Microbiology and Medical Zoology, Medical Sciences Campus, University of Puerto Rico, San Juan, Puerto RicoBackgroundPeriodontitis, one of the most common bacterial infections characterized by chronic inflammation, is also known to be a risk factor for chronic conditions, including cardiovascular disease and cancer. This inflammation is driven by an altered microbiota with an increase in pathogenic bacteria. We evaluated the association between oral microbiota and periodontitis severity in high-risk Hispanics.MethodThis cross-sectional study recruited 134 sexually active participants aged 21 to 49 years old from STI Clinics in Puerto Rico. A periodontal examination, saliva collection, and an interviewer-administered questionnaire were performed. Periodontal severity was categorized as: having no disease, mild, and moderate/severe and BOP and tooth loos was noted. Saliva samples were collected for genomic DNA extraction, downstream 16S rDNA amplification sequencing, and bioinformatics analyses.ResultsThe structure, composition, and diversity of bacterial communities differed significantly according to periodontal severity. The richness and overall diversity also differed between participants without periodontitis and participants with some level of periodontal disease. A higher abundance of Prevotella, Veillonella, or Treponema was attributed to periodontal disease and Aggregatibacter to severe bleeding on probing, while Neisseria was found in higher abundance in healthy participants, decreasing its levels with drinking, smoking, and oral sex practices.ConclusionsOur findings indicate that dysbiosis occurs as periodontal disease progresses, and both alcohol consumption and smoking habits pose risk factors for oral dysbiosis. These results are of public health and clinical impact, as several bacteria identified could serve in the future as biomarkers for periodontitis and oral cancer risk.https://www.frontiersin.org/articles/10.3389/fcimb.2022.965159/fulloral cavitymicrobiota16S rRNAperiodontitis (inflammatory)hispanics |
| spellingShingle | Ana P. Ortiz Ana P. Ortiz Kimil T. Acosta-Pagán Carla Oramas-Sepúlveda Maira A. Castañeda-Avila Brayan Vilanova-Cuevas Jeslie M. Ramos-Cartagena José A. Vivaldi Josué Pérez-Santiago Cynthia M. Pérez Filipa Godoy-Vitorino Oral microbiota and periodontitis severity among Hispanic adults Frontiers in Cellular and Infection Microbiology oral cavity microbiota 16S rRNA periodontitis (inflammatory) hispanics |
| title | Oral microbiota and periodontitis severity among Hispanic adults |
| title_full | Oral microbiota and periodontitis severity among Hispanic adults |
| title_fullStr | Oral microbiota and periodontitis severity among Hispanic adults |
| title_full_unstemmed | Oral microbiota and periodontitis severity among Hispanic adults |
| title_short | Oral microbiota and periodontitis severity among Hispanic adults |
| title_sort | oral microbiota and periodontitis severity among hispanic adults |
| topic | oral cavity microbiota 16S rRNA periodontitis (inflammatory) hispanics |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2022.965159/full |
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