The Effects of Helicobacter pylori Infection on Gastric Microbiota in Children With Duodenal Ulcer
BackgroundHelicobacter pylori (H. pylori) infection is the main cause of chronic gastritis and duodenal ulcer in children. Little is known about the effect of H. pylori on gastric microbiota in children with duodenal ulcer. This study is aimed at the characteristics of gastric microbiota in children...
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Frontiers Media S.A.
2022-04-01
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| Series: | Frontiers in Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmicb.2022.853184/full |
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| author | Wei Zheng Zhenya Zhu Jingjing Ying Gao Long Bo Chen Kerong Peng Fubang Li Hong Zhao Mizu Jiang Mizu Jiang |
| author_facet | Wei Zheng Zhenya Zhu Jingjing Ying Gao Long Bo Chen Kerong Peng Fubang Li Hong Zhao Mizu Jiang Mizu Jiang |
| author_sort | Wei Zheng |
| collection | DOAJ |
| description | BackgroundHelicobacter pylori (H. pylori) infection is the main cause of chronic gastritis and duodenal ulcer in children. Little is known about the effect of H. pylori on gastric microbiota in children with duodenal ulcer. This study is aimed at the characteristics of gastric microbiota in children with duodenal ulcer on H. pylori infection.MethodsWe studied 23 children diagnosed with duodenal ulcer by gastric endoscopy because of the gastrointestinal symptoms, 15 children were diagnosed with H. pylori infection, while 8 children were without H. pylori infection. Endoscopic mucosal biopsy samples were obtained for DNA extraction. Microbiomes were analyzed by 16S rRNA profiling and microbial functions were predicted using the software Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt).ResultsBacterial richness and diversity of gastric microbiota in duodenal ulcer with H. pylori-positive were lower than those negative. The gastric microbiota in H. pylori-positive group significantly reduced proportions of six phyla and fifteen genera; only Helicobacter taxa were more abundant in H. pylori-positive group. Co-expression network analysis showed a more complex network of interactions in the H. pylori-positive group than that in the H. pylori-negative group. For the predicted functions, lower abundance in the pathways of carbohydrate metabolism, signal transduction, amino acid metabolism, and lipid metabolism were found in H. pylori-positive group than the H. pylori-negative group. H. pylori colonization reduces a microbial community with genotoxic potential in the gastric mucosa of children with duodenal ulcer.ConclusionsThe presence of H. pylori significantly influences gastric microbiota and results in a lower abundance of multiple taxonomic levels in children with duodenal ulcer. Children with duodenal ulcer exhibit a dysbiotic microbial community with genotoxic potential, which is distinct from that of children with H. pylori infection.Clinical Trial Registration[http://www.chictr.org.cn], identifier [ChiCTR1800015190]. |
| first_indexed | 2024-04-14T01:20:19Z |
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| issn | 1664-302X |
| language | English |
| last_indexed | 2024-04-14T01:20:19Z |
| publishDate | 2022-04-01 |
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| series | Frontiers in Microbiology |
| spelling | doaj.art-173027ea83af43c996317c22846d114d2022-12-22T02:20:40ZengFrontiers Media S.A.Frontiers in Microbiology1664-302X2022-04-011310.3389/fmicb.2022.853184853184The Effects of Helicobacter pylori Infection on Gastric Microbiota in Children With Duodenal UlcerWei Zheng0Zhenya Zhu1Jingjing Ying2Gao Long3Bo Chen4Kerong Peng5Fubang Li6Hong Zhao7Mizu Jiang8Mizu Jiang9Department of Gastroenterology, Children’s Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health, National Children’s Regional Medical Center, Hangzhou, ChinaEndoscopy Center and Gastrointestinal Laboratory, Children’s Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health, National Children’s Regional Medical Center, Hangzhou, ChinaEndoscopy Center and Gastrointestinal Laboratory, Children’s Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health, National Children’s Regional Medical Center, Hangzhou, ChinaDepartment of Gastroenterology, Children’s Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health, National Children’s Regional Medical Center, Hangzhou, ChinaEndoscopy Center and Gastrointestinal Laboratory, Children’s Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health, National Children’s Regional Medical Center, Hangzhou, ChinaDepartment of Gastroenterology, Children’s Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health, National Children’s Regional Medical Center, Hangzhou, ChinaDepartment of Gastroenterology, Children’s Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health, National Children’s Regional Medical Center, Hangzhou, ChinaDepartment of Gastroenterology, Children’s Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health, National Children’s Regional Medical Center, Hangzhou, ChinaDepartment of Gastroenterology, Children’s Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health, National Children’s Regional Medical Center, Hangzhou, ChinaEndoscopy Center and Gastrointestinal Laboratory, Children’s Hospital, Zhejiang University School of Medicine, National Clinical Research Center for Child Health, National Children’s Regional Medical Center, Hangzhou, ChinaBackgroundHelicobacter pylori (H. pylori) infection is the main cause of chronic gastritis and duodenal ulcer in children. Little is known about the effect of H. pylori on gastric microbiota in children with duodenal ulcer. This study is aimed at the characteristics of gastric microbiota in children with duodenal ulcer on H. pylori infection.MethodsWe studied 23 children diagnosed with duodenal ulcer by gastric endoscopy because of the gastrointestinal symptoms, 15 children were diagnosed with H. pylori infection, while 8 children were without H. pylori infection. Endoscopic mucosal biopsy samples were obtained for DNA extraction. Microbiomes were analyzed by 16S rRNA profiling and microbial functions were predicted using the software Phylogenetic Investigation of Communities by Reconstruction of Unobserved States (PICRUSt).ResultsBacterial richness and diversity of gastric microbiota in duodenal ulcer with H. pylori-positive were lower than those negative. The gastric microbiota in H. pylori-positive group significantly reduced proportions of six phyla and fifteen genera; only Helicobacter taxa were more abundant in H. pylori-positive group. Co-expression network analysis showed a more complex network of interactions in the H. pylori-positive group than that in the H. pylori-negative group. For the predicted functions, lower abundance in the pathways of carbohydrate metabolism, signal transduction, amino acid metabolism, and lipid metabolism were found in H. pylori-positive group than the H. pylori-negative group. H. pylori colonization reduces a microbial community with genotoxic potential in the gastric mucosa of children with duodenal ulcer.ConclusionsThe presence of H. pylori significantly influences gastric microbiota and results in a lower abundance of multiple taxonomic levels in children with duodenal ulcer. Children with duodenal ulcer exhibit a dysbiotic microbial community with genotoxic potential, which is distinct from that of children with H. pylori infection.Clinical Trial Registration[http://www.chictr.org.cn], identifier [ChiCTR1800015190].https://www.frontiersin.org/articles/10.3389/fmicb.2022.853184/fullchildrenduodenal ulcergastric microbiotaHelicobacter pylori16S rRNA |
| spellingShingle | Wei Zheng Zhenya Zhu Jingjing Ying Gao Long Bo Chen Kerong Peng Fubang Li Hong Zhao Mizu Jiang Mizu Jiang The Effects of Helicobacter pylori Infection on Gastric Microbiota in Children With Duodenal Ulcer Frontiers in Microbiology children duodenal ulcer gastric microbiota Helicobacter pylori 16S rRNA |
| title | The Effects of Helicobacter pylori Infection on Gastric Microbiota in Children With Duodenal Ulcer |
| title_full | The Effects of Helicobacter pylori Infection on Gastric Microbiota in Children With Duodenal Ulcer |
| title_fullStr | The Effects of Helicobacter pylori Infection on Gastric Microbiota in Children With Duodenal Ulcer |
| title_full_unstemmed | The Effects of Helicobacter pylori Infection on Gastric Microbiota in Children With Duodenal Ulcer |
| title_short | The Effects of Helicobacter pylori Infection on Gastric Microbiota in Children With Duodenal Ulcer |
| title_sort | effects of helicobacter pylori infection on gastric microbiota in children with duodenal ulcer |
| topic | children duodenal ulcer gastric microbiota Helicobacter pylori 16S rRNA |
| url | https://www.frontiersin.org/articles/10.3389/fmicb.2022.853184/full |
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