A simple label-free electrochemical sensor for sensitive detection of alpha-fetoprotein based on specific aptamer immobilized platinum nanoparticles/carboxylated-graphene oxide
Abstract A label-free electrochemical aptamer-based sensor has been fabricated for alpha-fetoprotein (AFP) detection. Platinum nanoparticles on carboxylated-graphene oxide (PtNPs/GO-COOH) modified screen-printed graphene-carbon paste electrode (SPGE) was utilized as an immobilization platform, and t...
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| Format: | Article |
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Nature Portfolio
2021-07-01
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| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-021-93399-y |
| _version_ | 1818751805627039744 |
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| author | Jantima Upan Napaporn Youngvises Adisorn Tuantranont Chanpen Karuwan Philippe Banet Pierre-Henri Aubert Jaroon Jakmunee |
| author_facet | Jantima Upan Napaporn Youngvises Adisorn Tuantranont Chanpen Karuwan Philippe Banet Pierre-Henri Aubert Jaroon Jakmunee |
| author_sort | Jantima Upan |
| collection | DOAJ |
| description | Abstract A label-free electrochemical aptamer-based sensor has been fabricated for alpha-fetoprotein (AFP) detection. Platinum nanoparticles on carboxylated-graphene oxide (PtNPs/GO-COOH) modified screen-printed graphene-carbon paste electrode (SPGE) was utilized as an immobilization platform, and the AFP aptamer was employed as a bio-recognition element. The synthesized GO-COOH helps to increase the surface area and amounts of the immobilized aptamer. Subsequently, PtNPs are decorated on GO-COOH to enhance electrical conductivity and an oxidation current of the hydroquinone electrochemical probe. The aptamer selectively interacts with AFP, causing a decrease in the peak current of the hydroquinone because the binding biomolecules on the electrode surface hinder the electron transfer of the redox probe. Effects of aptamer concentration and AFP incubation time were studied, and the current changes of the redox probe before and after AFP binding were investigated by square wave voltammetry. The developed aptasensor provides a linear range from 3.0–30 ng mL−1 with a detection limit of 1.22 ng mL−1. Moreover, the aptamer immobilized electrode offers high selectivity to AFP molecules, good stability, and sensitive determination of AFP in human serum samples with high recoveries. |
| first_indexed | 2024-12-18T04:41:24Z |
| format | Article |
| id | doaj.art-1730a744eddd4c22bf553c11306ad94a |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-12-18T04:41:24Z |
| publishDate | 2021-07-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-1730a744eddd4c22bf553c11306ad94a2022-12-21T21:20:42ZengNature PortfolioScientific Reports2045-23222021-07-011111910.1038/s41598-021-93399-yA simple label-free electrochemical sensor for sensitive detection of alpha-fetoprotein based on specific aptamer immobilized platinum nanoparticles/carboxylated-graphene oxideJantima Upan0Napaporn Youngvises1Adisorn Tuantranont2Chanpen Karuwan3Philippe Banet4Pierre-Henri Aubert5Jaroon Jakmunee6Department of Chemistry, Faculty of Science, Chiang Mai UniversityThammasat University Research Unit in Carbon Materials and Green Chemistry Innovations, Department of Chemistry, Faculty of Science and Technology, Thammasat UniversityGraphene and Printed Electronics for Dual-Use Applications Research Division, National Security and Dual-Use Technology Center, National Science and Technology Development AgencyGraphene and Printed Electronics for Dual-Use Applications Research Division, National Security and Dual-Use Technology Center, National Science and Technology Development AgencyCY Cergy Paris Université, LPPICY Cergy Paris Université, LPPIDepartment of Chemistry, Faculty of Science, Chiang Mai UniversityAbstract A label-free electrochemical aptamer-based sensor has been fabricated for alpha-fetoprotein (AFP) detection. Platinum nanoparticles on carboxylated-graphene oxide (PtNPs/GO-COOH) modified screen-printed graphene-carbon paste electrode (SPGE) was utilized as an immobilization platform, and the AFP aptamer was employed as a bio-recognition element. The synthesized GO-COOH helps to increase the surface area and amounts of the immobilized aptamer. Subsequently, PtNPs are decorated on GO-COOH to enhance electrical conductivity and an oxidation current of the hydroquinone electrochemical probe. The aptamer selectively interacts with AFP, causing a decrease in the peak current of the hydroquinone because the binding biomolecules on the electrode surface hinder the electron transfer of the redox probe. Effects of aptamer concentration and AFP incubation time were studied, and the current changes of the redox probe before and after AFP binding were investigated by square wave voltammetry. The developed aptasensor provides a linear range from 3.0–30 ng mL−1 with a detection limit of 1.22 ng mL−1. Moreover, the aptamer immobilized electrode offers high selectivity to AFP molecules, good stability, and sensitive determination of AFP in human serum samples with high recoveries.https://doi.org/10.1038/s41598-021-93399-y |
| spellingShingle | Jantima Upan Napaporn Youngvises Adisorn Tuantranont Chanpen Karuwan Philippe Banet Pierre-Henri Aubert Jaroon Jakmunee A simple label-free electrochemical sensor for sensitive detection of alpha-fetoprotein based on specific aptamer immobilized platinum nanoparticles/carboxylated-graphene oxide Scientific Reports |
| title | A simple label-free electrochemical sensor for sensitive detection of alpha-fetoprotein based on specific aptamer immobilized platinum nanoparticles/carboxylated-graphene oxide |
| title_full | A simple label-free electrochemical sensor for sensitive detection of alpha-fetoprotein based on specific aptamer immobilized platinum nanoparticles/carboxylated-graphene oxide |
| title_fullStr | A simple label-free electrochemical sensor for sensitive detection of alpha-fetoprotein based on specific aptamer immobilized platinum nanoparticles/carboxylated-graphene oxide |
| title_full_unstemmed | A simple label-free electrochemical sensor for sensitive detection of alpha-fetoprotein based on specific aptamer immobilized platinum nanoparticles/carboxylated-graphene oxide |
| title_short | A simple label-free electrochemical sensor for sensitive detection of alpha-fetoprotein based on specific aptamer immobilized platinum nanoparticles/carboxylated-graphene oxide |
| title_sort | simple label free electrochemical sensor for sensitive detection of alpha fetoprotein based on specific aptamer immobilized platinum nanoparticles carboxylated graphene oxide |
| url | https://doi.org/10.1038/s41598-021-93399-y |
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