Comparative metabolite profiling of two rice genotypes with contrasting salt stress tolerance at the seedling stage.
BACKGROUND: Rice is sensitive to salt stress, especially at the seedling stage, with rice varieties differing remarkably in salt tolerance (ST). To understand the physiological mechanisms of ST, we investigated salt stress responses at the metabolite level. METHODS: Gas chromatography-mass spectrome...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Public Library of Science (PLoS)
2014-01-01
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| Series: | PLoS ONE |
| Online Access: | http://europepmc.org/articles/PMC4179258?pdf=render |
| _version_ | 1818179363492855808 |
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| author | Xiuqin Zhao Wensheng Wang Fan Zhang Jianli Deng Zhikang Li Binying Fu |
| author_facet | Xiuqin Zhao Wensheng Wang Fan Zhang Jianli Deng Zhikang Li Binying Fu |
| author_sort | Xiuqin Zhao |
| collection | DOAJ |
| description | BACKGROUND: Rice is sensitive to salt stress, especially at the seedling stage, with rice varieties differing remarkably in salt tolerance (ST). To understand the physiological mechanisms of ST, we investigated salt stress responses at the metabolite level. METHODS: Gas chromatography-mass spectrometry was used to profile metabolite changes in the salt-tolerant line FL478 and the sensitive variety IR64 under a salt-stress time series. Additionally, several physiological traits related to ST were investigated. RESULTS: We characterized 92 primary metabolites in the leaves and roots of the two genotypes under stress and control conditions. The metabolites were temporally, tissue-specifically and genotype-dependently regulated under salt stress. Sugars and amino acids (AAs) increased significantly in the leaves and roots of both genotypes, while organic acids (OAs) increased in roots and decreased in leaves. Compared with IR64, FL478 experienced greater increases in sugars and AAs and more pronounced decreases in OAs in both tissues; additionally, the maximum change in sugars and AAs occurred later, while OAs changed earlier. Moreover, less Na+ and higher relative water content were observed in FL478. Eleven metabolites, including AAs and sugars, were specifically increased in FL478 over the course of the treatment. CONCLUSIONS: Metabolic responses of rice to salt stress are dynamic and involve many metabolites. The greater ST of FL478 is due to different adaptive reactions at different stress times. At early salt-stress stages, FL478 adapts to stress by decreasing OA levels or by quickly depressing growth; during later stages, more metabolites are accumulated, thereby serving as compatible solutes against osmotic challenge induced by salt stress. |
| first_indexed | 2024-12-11T21:02:41Z |
| format | Article |
| id | doaj.art-1731b9b42a4f4c62bbe036af7dbc3f6c |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-11T21:02:41Z |
| publishDate | 2014-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-1731b9b42a4f4c62bbe036af7dbc3f6c2022-12-22T00:50:57ZengPublic Library of Science (PLoS)PLoS ONE1932-62032014-01-0199e10802010.1371/journal.pone.0108020Comparative metabolite profiling of two rice genotypes with contrasting salt stress tolerance at the seedling stage.Xiuqin ZhaoWensheng WangFan ZhangJianli DengZhikang LiBinying FuBACKGROUND: Rice is sensitive to salt stress, especially at the seedling stage, with rice varieties differing remarkably in salt tolerance (ST). To understand the physiological mechanisms of ST, we investigated salt stress responses at the metabolite level. METHODS: Gas chromatography-mass spectrometry was used to profile metabolite changes in the salt-tolerant line FL478 and the sensitive variety IR64 under a salt-stress time series. Additionally, several physiological traits related to ST were investigated. RESULTS: We characterized 92 primary metabolites in the leaves and roots of the two genotypes under stress and control conditions. The metabolites were temporally, tissue-specifically and genotype-dependently regulated under salt stress. Sugars and amino acids (AAs) increased significantly in the leaves and roots of both genotypes, while organic acids (OAs) increased in roots and decreased in leaves. Compared with IR64, FL478 experienced greater increases in sugars and AAs and more pronounced decreases in OAs in both tissues; additionally, the maximum change in sugars and AAs occurred later, while OAs changed earlier. Moreover, less Na+ and higher relative water content were observed in FL478. Eleven metabolites, including AAs and sugars, were specifically increased in FL478 over the course of the treatment. CONCLUSIONS: Metabolic responses of rice to salt stress are dynamic and involve many metabolites. The greater ST of FL478 is due to different adaptive reactions at different stress times. At early salt-stress stages, FL478 adapts to stress by decreasing OA levels or by quickly depressing growth; during later stages, more metabolites are accumulated, thereby serving as compatible solutes against osmotic challenge induced by salt stress.http://europepmc.org/articles/PMC4179258?pdf=render |
| spellingShingle | Xiuqin Zhao Wensheng Wang Fan Zhang Jianli Deng Zhikang Li Binying Fu Comparative metabolite profiling of two rice genotypes with contrasting salt stress tolerance at the seedling stage. PLoS ONE |
| title | Comparative metabolite profiling of two rice genotypes with contrasting salt stress tolerance at the seedling stage. |
| title_full | Comparative metabolite profiling of two rice genotypes with contrasting salt stress tolerance at the seedling stage. |
| title_fullStr | Comparative metabolite profiling of two rice genotypes with contrasting salt stress tolerance at the seedling stage. |
| title_full_unstemmed | Comparative metabolite profiling of two rice genotypes with contrasting salt stress tolerance at the seedling stage. |
| title_short | Comparative metabolite profiling of two rice genotypes with contrasting salt stress tolerance at the seedling stage. |
| title_sort | comparative metabolite profiling of two rice genotypes with contrasting salt stress tolerance at the seedling stage |
| url | http://europepmc.org/articles/PMC4179258?pdf=render |
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