Nano-molecularly imprinted polymers for serum creatinine sensing using the heat transfer method
Serum creatinine concentration is an important clinical measure of kidney function. However, standard methods of detection, such as the Jaffe method or enzymatic assays, suffer several disadvantages, including non-specificity and procedural complexity, or high cost, respectively. In this work, we pr...
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-08-01
|
| Series: | Talanta Open |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666831922000066 |
| _version_ | 1818234760768520192 |
|---|---|
| author | Rhys J. Williams Robert D. Crapnell Nina C. Dempsey Marloes Peeters Craig E. Banks |
| author_facet | Rhys J. Williams Robert D. Crapnell Nina C. Dempsey Marloes Peeters Craig E. Banks |
| author_sort | Rhys J. Williams |
| collection | DOAJ |
| description | Serum creatinine concentration is an important clinical measure of kidney function. However, standard methods of detection, such as the Jaffe method or enzymatic assays, suffer several disadvantages, including non-specificity and procedural complexity, or high cost, respectively. In this work, we propose the use of nano-molecularly imprinted polymers (nMIPs) in conjunction with the novel Heat Transfer Method (HTM) as a promising alternative sensing platform to these existing methods for measuring serum creatinine concentration. More specifically, it is shown that creatinine-imprinted nMIPs can be produced using a solid-phase templating method, and that simple drop-casting onto a cheap, disposable substrate can be used in conjunction with HTM to detect creatinine with a limit-of-detection of (7.0 ± 0.5) μM in buffer solutions. Furthermore, the nMIPs are shown to selectively bind creatinine in comparison to several similar molecules, and the sensing platform is demonstrated to be able to detect changes in creatinine concentration in complex blood plasma samples. |
| first_indexed | 2024-12-12T11:43:12Z |
| format | Article |
| id | doaj.art-17334a77417345c7afda92e55a886a08 |
| institution | Directory Open Access Journal |
| issn | 2666-8319 |
| language | English |
| last_indexed | 2024-12-12T11:43:12Z |
| publishDate | 2022-08-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Talanta Open |
| spelling | doaj.art-17334a77417345c7afda92e55a886a082022-12-22T00:25:29ZengElsevierTalanta Open2666-83192022-08-015100087Nano-molecularly imprinted polymers for serum creatinine sensing using the heat transfer methodRhys J. Williams0Robert D. Crapnell1Nina C. Dempsey2Marloes Peeters3Craig E. Banks4Faculty of Science and Engineering, Manchester Metropolitan University, Chester Street, Manchester M1 5GD, United KingdomFaculty of Science and Engineering, Manchester Metropolitan University, Chester Street, Manchester M1 5GD, United KingdomFaculty of Science and Engineering, Manchester Metropolitan University, Chester Street, Manchester M1 5GD, United KingdomSchool of Engineering, Newcastle University, Merz Court, Newcastle upon Tyne NE1 7RU, United KingdomFaculty of Science and Engineering, Manchester Metropolitan University, Chester Street, Manchester M1 5GD, United Kingdom; Corresponding author.Serum creatinine concentration is an important clinical measure of kidney function. However, standard methods of detection, such as the Jaffe method or enzymatic assays, suffer several disadvantages, including non-specificity and procedural complexity, or high cost, respectively. In this work, we propose the use of nano-molecularly imprinted polymers (nMIPs) in conjunction with the novel Heat Transfer Method (HTM) as a promising alternative sensing platform to these existing methods for measuring serum creatinine concentration. More specifically, it is shown that creatinine-imprinted nMIPs can be produced using a solid-phase templating method, and that simple drop-casting onto a cheap, disposable substrate can be used in conjunction with HTM to detect creatinine with a limit-of-detection of (7.0 ± 0.5) μM in buffer solutions. Furthermore, the nMIPs are shown to selectively bind creatinine in comparison to several similar molecules, and the sensing platform is demonstrated to be able to detect changes in creatinine concentration in complex blood plasma samples.http://www.sciencedirect.com/science/article/pii/S2666831922000066Serum creatinine sensingSolid-phase templatingNano-molecularly imprinted polymerHeat transfer method |
| spellingShingle | Rhys J. Williams Robert D. Crapnell Nina C. Dempsey Marloes Peeters Craig E. Banks Nano-molecularly imprinted polymers for serum creatinine sensing using the heat transfer method Talanta Open Serum creatinine sensing Solid-phase templating Nano-molecularly imprinted polymer Heat transfer method |
| title | Nano-molecularly imprinted polymers for serum creatinine sensing using the heat transfer method |
| title_full | Nano-molecularly imprinted polymers for serum creatinine sensing using the heat transfer method |
| title_fullStr | Nano-molecularly imprinted polymers for serum creatinine sensing using the heat transfer method |
| title_full_unstemmed | Nano-molecularly imprinted polymers for serum creatinine sensing using the heat transfer method |
| title_short | Nano-molecularly imprinted polymers for serum creatinine sensing using the heat transfer method |
| title_sort | nano molecularly imprinted polymers for serum creatinine sensing using the heat transfer method |
| topic | Serum creatinine sensing Solid-phase templating Nano-molecularly imprinted polymer Heat transfer method |
| url | http://www.sciencedirect.com/science/article/pii/S2666831922000066 |
| work_keys_str_mv | AT rhysjwilliams nanomolecularlyimprintedpolymersforserumcreatininesensingusingtheheattransfermethod AT robertdcrapnell nanomolecularlyimprintedpolymersforserumcreatininesensingusingtheheattransfermethod AT ninacdempsey nanomolecularlyimprintedpolymersforserumcreatininesensingusingtheheattransfermethod AT marloespeeters nanomolecularlyimprintedpolymersforserumcreatininesensingusingtheheattransfermethod AT craigebanks nanomolecularlyimprintedpolymersforserumcreatininesensingusingtheheattransfermethod |