Data on isolating mesenchymal stromal cells from human adipose tissue using a collagenase-free method
The present dataset describes a detailed protocol to isolate mesenchymal cells from human fat without the use of collagenase. Human fat specimen, surgically cleaned from non-fat tissues (e.g., blood vessels) and reduced into smaller fat pieces of around 1–3 mm size, is incubated in complete culture...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2016-03-01
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| Series: | Data in Brief |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2352340916300245 |
| _version_ | 1818174047610994688 |
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| author | Wassim Shebaby Eddie K. Abdalla Fady Saad Wissam H. Faour |
| author_facet | Wassim Shebaby Eddie K. Abdalla Fady Saad Wissam H. Faour |
| author_sort | Wassim Shebaby |
| collection | DOAJ |
| description | The present dataset describes a detailed protocol to isolate mesenchymal cells from human fat without the use of collagenase. Human fat specimen, surgically cleaned from non-fat tissues (e.g., blood vessels) and reduced into smaller fat pieces of around 1–3 mm size, is incubated in complete culture media for five to seven days. Then, cells started to spread out from the fat explants and to grow in cultures according to an exponential pattern. Our data showed that primary mesenchymal cells presenting heterogeneous morphology start to acquire more homogenous fibroblastic-like shape when cultured for longer duration or when subcultured into new flasks. Cell isolation efficiency as well as cell doubling time were also calculated throughout the culturing experimentations and illustrated in a separate figure thereafter. This paper contains data previously considered as an alternative protocol to isolate adipose-derived mesenchymal stem cell published in “Proliferation and differentiation of human adipose-derived mesenchymal stem cells (ASCs) into osteoblastic lineage are passage dependent” [1]. Keywords: Adipose tissue, mesenchymal stromal cell, cell culture, doubling time |
| first_indexed | 2024-12-11T19:38:11Z |
| format | Article |
| id | doaj.art-17372b9160c6446dbab3673044e5ded1 |
| institution | Directory Open Access Journal |
| issn | 2352-3409 |
| language | English |
| last_indexed | 2024-12-11T19:38:11Z |
| publishDate | 2016-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Data in Brief |
| spelling | doaj.art-17372b9160c6446dbab3673044e5ded12022-12-22T00:53:06ZengElsevierData in Brief2352-34092016-03-016974979Data on isolating mesenchymal stromal cells from human adipose tissue using a collagenase-free methodWassim Shebaby0Eddie K. Abdalla1Fady Saad2Wissam H. Faour3Department of Natural Sciences, School of Arts & Sciences, Lebanese American University, Byblos, Lebanon, P.O. Box 36, LebanonLebanese American University Medical Center – Rizk Hospital, Beirut, LebanonSaint Louis Hospital, Jounieh, LebanonSchool of Medicine, Lebanese American University, Byblos, Lebanon, P.O. Box 36, Lebanon; Corresponding author. Tel. +961 9547262x2396; fax: +961 9546262.The present dataset describes a detailed protocol to isolate mesenchymal cells from human fat without the use of collagenase. Human fat specimen, surgically cleaned from non-fat tissues (e.g., blood vessels) and reduced into smaller fat pieces of around 1–3 mm size, is incubated in complete culture media for five to seven days. Then, cells started to spread out from the fat explants and to grow in cultures according to an exponential pattern. Our data showed that primary mesenchymal cells presenting heterogeneous morphology start to acquire more homogenous fibroblastic-like shape when cultured for longer duration or when subcultured into new flasks. Cell isolation efficiency as well as cell doubling time were also calculated throughout the culturing experimentations and illustrated in a separate figure thereafter. This paper contains data previously considered as an alternative protocol to isolate adipose-derived mesenchymal stem cell published in “Proliferation and differentiation of human adipose-derived mesenchymal stem cells (ASCs) into osteoblastic lineage are passage dependent” [1]. Keywords: Adipose tissue, mesenchymal stromal cell, cell culture, doubling timehttp://www.sciencedirect.com/science/article/pii/S2352340916300245 |
| spellingShingle | Wassim Shebaby Eddie K. Abdalla Fady Saad Wissam H. Faour Data on isolating mesenchymal stromal cells from human adipose tissue using a collagenase-free method Data in Brief |
| title | Data on isolating mesenchymal stromal cells from human adipose tissue using a collagenase-free method |
| title_full | Data on isolating mesenchymal stromal cells from human adipose tissue using a collagenase-free method |
| title_fullStr | Data on isolating mesenchymal stromal cells from human adipose tissue using a collagenase-free method |
| title_full_unstemmed | Data on isolating mesenchymal stromal cells from human adipose tissue using a collagenase-free method |
| title_short | Data on isolating mesenchymal stromal cells from human adipose tissue using a collagenase-free method |
| title_sort | data on isolating mesenchymal stromal cells from human adipose tissue using a collagenase free method |
| url | http://www.sciencedirect.com/science/article/pii/S2352340916300245 |
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