Atomic force microscopy fishing and mass spectrometry identification of gp120 on immobilized aptamers
Yuri D Ivanov,1 Natalia S Bukharina,1 Tatyana O Pleshakova,1 Pavel A Frantsuzov,1 Elena Yu Andreeva,1 Anna L Kaysheva,1,2 Victor G Zgoda,1 Alexander A Izotov,1 Tatyana I Pavlova,1 Vadim S Ziborov,1 Sergey P Radko,1 Sergei A Moshkovskii,1 Alexander I Archakov1 1Department of Personalized Medicine, O...
| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Dove Medical Press
2014-10-01
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| Series: | International Journal of Nanomedicine |
| Online Access: | http://www.dovepress.com/atomic-force-microscopy-fishing-and-mass-spectrometry-identification-o-peer-reviewed-article-IJN |
| _version_ | 1818406537352183808 |
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| author | Ivanov YD Bukharina NS Pleshakova TO Frantsuzov PA Andreeva EY Kaysheva AL Zgoda VG Izotov AA Pavlova TI Ziborov VS Radko SP Moshkovskii SA Archakov AI |
| author_facet | Ivanov YD Bukharina NS Pleshakova TO Frantsuzov PA Andreeva EY Kaysheva AL Zgoda VG Izotov AA Pavlova TI Ziborov VS Radko SP Moshkovskii SA Archakov AI |
| author_sort | Ivanov YD |
| collection | DOAJ |
| description | Yuri D Ivanov,1 Natalia S Bukharina,1 Tatyana O Pleshakova,1 Pavel A Frantsuzov,1 Elena Yu Andreeva,1 Anna L Kaysheva,1,2 Victor G Zgoda,1 Alexander A Izotov,1 Tatyana I Pavlova,1 Vadim S Ziborov,1 Sergey P Radko,1 Sergei A Moshkovskii,1 Alexander I Archakov1 1Department of Personalized Medicine, Orekhovich Institute of Biomedical Chemistry of the Russian Academy of Medical Sciences, Moscow, Russia; 2PostgenTech Ltd., Moscow, Russia Abstract: Atomic force microscopy (AFM) was applied to carry out direct and label-free detection of gp120 human immunodeficiency virus type 1 envelope glycoprotein as a target protein. This approach was based on the AFM fishing of gp120 from the analyte solution using anti-gp120 aptamers immobilized on the AFM chip to count gp120/aptamer complexes that were formed on the chip surface. The comparison of image contrasts of fished gp120 against the background of immobilized aptamers and anti-gp120 antibodies on the AFM images was conducted. It was shown that an image contrast of the protein/aptamer complexes was two-fold higher than the contrast of the protein/antibody complexes. Mass spectrometry identification provided an additional confirmation of the target protein presence on the AFM chips after biospecific fishing to avoid any artifacts. Keywords: gp120 HIV-1 envelope glycoprotein, aptamer, atomic force microscopy, mass spectrometry |
| first_indexed | 2024-12-14T09:13:31Z |
| format | Article |
| id | doaj.art-1737ed45e92e4620ba75b1a6a7c9e15c |
| institution | Directory Open Access Journal |
| issn | 1178-2013 |
| language | English |
| last_indexed | 2024-12-14T09:13:31Z |
| publishDate | 2014-10-01 |
| publisher | Dove Medical Press |
| record_format | Article |
| series | International Journal of Nanomedicine |
| spelling | doaj.art-1737ed45e92e4620ba75b1a6a7c9e15c2022-12-21T23:08:30ZengDove Medical PressInternational Journal of Nanomedicine1178-20132014-10-012014Issue 14659467018607Atomic force microscopy fishing and mass spectrometry identification of gp120 on immobilized aptamersIvanov YDBukharina NSPleshakova TOFrantsuzov PAAndreeva EYKaysheva ALZgoda VGIzotov AAPavlova TIZiborov VSRadko SPMoshkovskii SAArchakov AIYuri D Ivanov,1 Natalia S Bukharina,1 Tatyana O Pleshakova,1 Pavel A Frantsuzov,1 Elena Yu Andreeva,1 Anna L Kaysheva,1,2 Victor G Zgoda,1 Alexander A Izotov,1 Tatyana I Pavlova,1 Vadim S Ziborov,1 Sergey P Radko,1 Sergei A Moshkovskii,1 Alexander I Archakov1 1Department of Personalized Medicine, Orekhovich Institute of Biomedical Chemistry of the Russian Academy of Medical Sciences, Moscow, Russia; 2PostgenTech Ltd., Moscow, Russia Abstract: Atomic force microscopy (AFM) was applied to carry out direct and label-free detection of gp120 human immunodeficiency virus type 1 envelope glycoprotein as a target protein. This approach was based on the AFM fishing of gp120 from the analyte solution using anti-gp120 aptamers immobilized on the AFM chip to count gp120/aptamer complexes that were formed on the chip surface. The comparison of image contrasts of fished gp120 against the background of immobilized aptamers and anti-gp120 antibodies on the AFM images was conducted. It was shown that an image contrast of the protein/aptamer complexes was two-fold higher than the contrast of the protein/antibody complexes. Mass spectrometry identification provided an additional confirmation of the target protein presence on the AFM chips after biospecific fishing to avoid any artifacts. Keywords: gp120 HIV-1 envelope glycoprotein, aptamer, atomic force microscopy, mass spectrometryhttp://www.dovepress.com/atomic-force-microscopy-fishing-and-mass-spectrometry-identification-o-peer-reviewed-article-IJN |
| spellingShingle | Ivanov YD Bukharina NS Pleshakova TO Frantsuzov PA Andreeva EY Kaysheva AL Zgoda VG Izotov AA Pavlova TI Ziborov VS Radko SP Moshkovskii SA Archakov AI Atomic force microscopy fishing and mass spectrometry identification of gp120 on immobilized aptamers International Journal of Nanomedicine |
| title | Atomic force microscopy fishing and mass spectrometry identification of gp120 on immobilized aptamers |
| title_full | Atomic force microscopy fishing and mass spectrometry identification of gp120 on immobilized aptamers |
| title_fullStr | Atomic force microscopy fishing and mass spectrometry identification of gp120 on immobilized aptamers |
| title_full_unstemmed | Atomic force microscopy fishing and mass spectrometry identification of gp120 on immobilized aptamers |
| title_short | Atomic force microscopy fishing and mass spectrometry identification of gp120 on immobilized aptamers |
| title_sort | atomic force microscopy fishing and mass spectrometry identification of gp120 on immobilized aptamers |
| url | http://www.dovepress.com/atomic-force-microscopy-fishing-and-mass-spectrometry-identification-o-peer-reviewed-article-IJN |
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