In Vitro Assessment of Antiproliferative Activity and Cytotoxicity Modulation of <i>Capsicum chinense</i> By-Product Extracts
<i>Capsicum chinense</i> J., also known locally as habanero pepper, is a medicinal herb known for its pharmacological properties. Its properties are attributed to the capsaicinoids and polyphenols found in its fruit and polyphenols in its by-products. The anticancer potential of <i>...
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MDPI AG
2022-06-01
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| Online Access: | https://www.mdpi.com/2076-3417/12/12/5818 |
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| author | Lilian Dolores Chel-Guerrero Matteo Scampicchio Giovanna Ferrentino Ingrid Mayanín Rodríguez-Buenfil Mabel Fragoso-Serrano |
| author_facet | Lilian Dolores Chel-Guerrero Matteo Scampicchio Giovanna Ferrentino Ingrid Mayanín Rodríguez-Buenfil Mabel Fragoso-Serrano |
| author_sort | Lilian Dolores Chel-Guerrero |
| collection | DOAJ |
| description | <i>Capsicum chinense</i> J., also known locally as habanero pepper, is a medicinal herb known for its pharmacological properties. Its properties are attributed to the capsaicinoids and polyphenols found in its fruit and polyphenols in its by-products. The anticancer potential of <i>C. chinense</i> by-products remains unexplored. This study aimed to evaluate the antiproliferative activity and modulation of the cytotoxicity of extracts obtained from <i>C. chinense</i> by-products of plants grown on black and red soils of Yucatan, Mexico. Dry by-product extracts were obtained using maceration, a Soxhlet, and supercritical fluid extraction. In vitro antiproliferative activity and cytotoxicity modulation were evaluated by the sulforhodamine B method. The extract of leaves of plants grown on black soil obtained by maceration displayed selective high cytotoxicity against colorectal cancer cells, IC<sub>50</sub> HCT–15 = 16.23 ± 2.89 µg mL<sup>−1</sup>. The leaf and stem extracts of plants grown on red soil obtained by maceration potentiated the vinblastine’s effect against parental breast cancer cells, MCF–7/Sens, with a reversion factor of 362.50-fold. Additionally, the extract of stems from plants grown on black soil obtained by supercritical fluid extraction and all the by-product extracts from plants grown on black soil obtained through maceration increased the effect of vinblastine against MCF–7/Vin<sup>+</sup> with a reversion factor from 5.06- to 7.78-fold. These results highlight the anticancer potential of <i>C. chinense</i> by-products. |
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| last_indexed | 2024-03-10T00:32:10Z |
| publishDate | 2022-06-01 |
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| spelling | doaj.art-1738cda88df64dfd954dd9bad604c98c2023-11-23T15:22:52ZengMDPI AGApplied Sciences2076-34172022-06-011212581810.3390/app12125818In Vitro Assessment of Antiproliferative Activity and Cytotoxicity Modulation of <i>Capsicum chinense</i> By-Product ExtractsLilian Dolores Chel-Guerrero0Matteo Scampicchio1Giovanna Ferrentino2Ingrid Mayanín Rodríguez-Buenfil3Mabel Fragoso-Serrano4Centro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco A.C. Subsede Sureste, Tablaje Catastral 31264 Km, 5.5 Carretera Sierra Papacal-Chuburna Puerto Parque Científico Tecnológico de Yucatan, Merida 97302, MexicoFaculty of Science and Technology, Free University of Bozen-Bolzano, Piazza Università 5, 39100 Bolzano, ItalyFaculty of Science and Technology, Free University of Bozen-Bolzano, Piazza Università 5, 39100 Bolzano, ItalyCentro de Investigación y Asistencia en Tecnología y Diseño del Estado de Jalisco A.C. Subsede Sureste, Tablaje Catastral 31264 Km, 5.5 Carretera Sierra Papacal-Chuburna Puerto Parque Científico Tecnológico de Yucatan, Merida 97302, MexicoDepartamento de Farmacia, Facultad de Química, Universidad Nacional Autónoma de Mexico (UNAM), Ciudad Universitaria, Ciudad de Mexico 04510, Mexico<i>Capsicum chinense</i> J., also known locally as habanero pepper, is a medicinal herb known for its pharmacological properties. Its properties are attributed to the capsaicinoids and polyphenols found in its fruit and polyphenols in its by-products. The anticancer potential of <i>C. chinense</i> by-products remains unexplored. This study aimed to evaluate the antiproliferative activity and modulation of the cytotoxicity of extracts obtained from <i>C. chinense</i> by-products of plants grown on black and red soils of Yucatan, Mexico. Dry by-product extracts were obtained using maceration, a Soxhlet, and supercritical fluid extraction. In vitro antiproliferative activity and cytotoxicity modulation were evaluated by the sulforhodamine B method. The extract of leaves of plants grown on black soil obtained by maceration displayed selective high cytotoxicity against colorectal cancer cells, IC<sub>50</sub> HCT–15 = 16.23 ± 2.89 µg mL<sup>−1</sup>. The leaf and stem extracts of plants grown on red soil obtained by maceration potentiated the vinblastine’s effect against parental breast cancer cells, MCF–7/Sens, with a reversion factor of 362.50-fold. Additionally, the extract of stems from plants grown on black soil obtained by supercritical fluid extraction and all the by-product extracts from plants grown on black soil obtained through maceration increased the effect of vinblastine against MCF–7/Vin<sup>+</sup> with a reversion factor from 5.06- to 7.78-fold. These results highlight the anticancer potential of <i>C. chinense</i> by-products.https://www.mdpi.com/2076-3417/12/12/5818habaneroby-productscytotoxicitymodulationsensible and resistant MCF–7supercritical fluid extraction |
| spellingShingle | Lilian Dolores Chel-Guerrero Matteo Scampicchio Giovanna Ferrentino Ingrid Mayanín Rodríguez-Buenfil Mabel Fragoso-Serrano In Vitro Assessment of Antiproliferative Activity and Cytotoxicity Modulation of <i>Capsicum chinense</i> By-Product Extracts Applied Sciences habanero by-products cytotoxicity modulation sensible and resistant MCF–7 supercritical fluid extraction |
| title | In Vitro Assessment of Antiproliferative Activity and Cytotoxicity Modulation of <i>Capsicum chinense</i> By-Product Extracts |
| title_full | In Vitro Assessment of Antiproliferative Activity and Cytotoxicity Modulation of <i>Capsicum chinense</i> By-Product Extracts |
| title_fullStr | In Vitro Assessment of Antiproliferative Activity and Cytotoxicity Modulation of <i>Capsicum chinense</i> By-Product Extracts |
| title_full_unstemmed | In Vitro Assessment of Antiproliferative Activity and Cytotoxicity Modulation of <i>Capsicum chinense</i> By-Product Extracts |
| title_short | In Vitro Assessment of Antiproliferative Activity and Cytotoxicity Modulation of <i>Capsicum chinense</i> By-Product Extracts |
| title_sort | in vitro assessment of antiproliferative activity and cytotoxicity modulation of i capsicum chinense i by product extracts |
| topic | habanero by-products cytotoxicity modulation sensible and resistant MCF–7 supercritical fluid extraction |
| url | https://www.mdpi.com/2076-3417/12/12/5818 |
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