SHMT2 regulates serine metabolism to promote the progression and immunosuppression of papillary renal cell carcinoma
Recent research has demonstrated the diverse relationship between tumour metabolism and the tumour microenvironment (TME), for example, abnormal serine metabolism. This study investigated the role of serine metabolism in papillary renal cell carcinoma (pRCC) focusing on the prognostic value and regu...
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| Format: | Article |
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Frontiers Media S.A.
2022-08-01
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| Series: | Frontiers in Oncology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fonc.2022.914332/full |
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| author | Weiyu Kong Weiyu Kong Zhongyuan Wang Nuoran Chen Yiwen Mei Yang Li Yulin Yue |
| author_facet | Weiyu Kong Weiyu Kong Zhongyuan Wang Nuoran Chen Yiwen Mei Yang Li Yulin Yue |
| author_sort | Weiyu Kong |
| collection | DOAJ |
| description | Recent research has demonstrated the diverse relationship between tumour metabolism and the tumour microenvironment (TME), for example, abnormal serine metabolism. This study investigated the role of serine metabolism in papillary renal cell carcinoma (pRCC) focusing on the prognostic value and regulatory mechanisms. Gene expression profiles and clinical data of patients with pRCC were obtained from The Cancer Genome Atlas (TCGA) database and Gene Expression Omnibus (GEO) database. Kaplan–Meier curves were used for survival analysis and consensus clustering for tumour serine metabolic signatures extraction. Functional analysis, including the Kyoto Encyclopedia of Genes and Genomes (KEGG) and gene set enrichment analysis (GSEA), was applied to explore the biological characteristics. The gene set variation analysis (GSVA), single-sample GSEA (ssGSEA), and Estimation of Stromal and Immune cells in Malignant Tumour tissues using Expression data (ESTIMATE) methods were utilised to estimate the immune infiltration in the various subtypes. Five serine metabolic genes (SMGs) were used to classify patients with pRCC, with four clusters identified with diverse prognoses and immune features based on these survival-related SMGs. Further analysis of the best and worst clusters (B and D clusters) revealed variations in survival, clinical progression, oncogenic pathways, and TME, which included immune infiltration scores, immunosuppressive cell infiltration, and expression of immune checkpoints. In addition, SMGs, especially SHMT2, exacerbated the carcinogenesis and immunosuppressive cells in pRCC, thus promoting tumour proliferation. In conclusion, higher SHMT2 gene expression and higher serine metabolism in tumour cells are associated with poorer clinical outcomes in pRCC. SHMT2 is a potential novel target gene for targeted therapy and immunotherapy in pRCC. |
| first_indexed | 2024-12-10T18:01:58Z |
| format | Article |
| id | doaj.art-173c5717dc864415addc104912b49a43 |
| institution | Directory Open Access Journal |
| issn | 2234-943X |
| language | English |
| last_indexed | 2024-12-10T18:01:58Z |
| publishDate | 2022-08-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Oncology |
| spelling | doaj.art-173c5717dc864415addc104912b49a432022-12-22T01:38:44ZengFrontiers Media S.A.Frontiers in Oncology2234-943X2022-08-011210.3389/fonc.2022.914332914332SHMT2 regulates serine metabolism to promote the progression and immunosuppression of papillary renal cell carcinomaWeiyu Kong0Weiyu Kong1Zhongyuan Wang2Nuoran Chen3Yiwen Mei4Yang Li5Yulin Yue6Department of Clinical Laboratory, Children’s Hospital of Nanjing Medical University, Nanjing, ChinaFirst Clinical Medical College of Nanjing Medical University, Nanjing, ChinaThe State Key Lab of Reproductive, Department of Urology, The First Affiliated Hospital of Nanjing Medical University, Nanjing, ChinaFirst Clinical Medical College of Nanjing Medical University, Nanjing, ChinaFirst Clinical Medical College of Nanjing Medical University, Nanjing, ChinaDepartment of Urology, Shanghai Pudong Hospital, Fudan University Pudong Medical Center, Shanghai, ChinaDepartment of Clinical Laboratory, Children’s Hospital of Nanjing Medical University, Nanjing, ChinaRecent research has demonstrated the diverse relationship between tumour metabolism and the tumour microenvironment (TME), for example, abnormal serine metabolism. This study investigated the role of serine metabolism in papillary renal cell carcinoma (pRCC) focusing on the prognostic value and regulatory mechanisms. Gene expression profiles and clinical data of patients with pRCC were obtained from The Cancer Genome Atlas (TCGA) database and Gene Expression Omnibus (GEO) database. Kaplan–Meier curves were used for survival analysis and consensus clustering for tumour serine metabolic signatures extraction. Functional analysis, including the Kyoto Encyclopedia of Genes and Genomes (KEGG) and gene set enrichment analysis (GSEA), was applied to explore the biological characteristics. The gene set variation analysis (GSVA), single-sample GSEA (ssGSEA), and Estimation of Stromal and Immune cells in Malignant Tumour tissues using Expression data (ESTIMATE) methods were utilised to estimate the immune infiltration in the various subtypes. Five serine metabolic genes (SMGs) were used to classify patients with pRCC, with four clusters identified with diverse prognoses and immune features based on these survival-related SMGs. Further analysis of the best and worst clusters (B and D clusters) revealed variations in survival, clinical progression, oncogenic pathways, and TME, which included immune infiltration scores, immunosuppressive cell infiltration, and expression of immune checkpoints. In addition, SMGs, especially SHMT2, exacerbated the carcinogenesis and immunosuppressive cells in pRCC, thus promoting tumour proliferation. In conclusion, higher SHMT2 gene expression and higher serine metabolism in tumour cells are associated with poorer clinical outcomes in pRCC. SHMT2 is a potential novel target gene for targeted therapy and immunotherapy in pRCC.https://www.frontiersin.org/articles/10.3389/fonc.2022.914332/fullcancer metabolismserinetumour microenvironmentSHMT2immunotherapy |
| spellingShingle | Weiyu Kong Weiyu Kong Zhongyuan Wang Nuoran Chen Yiwen Mei Yang Li Yulin Yue SHMT2 regulates serine metabolism to promote the progression and immunosuppression of papillary renal cell carcinoma Frontiers in Oncology cancer metabolism serine tumour microenvironment SHMT2 immunotherapy |
| title | SHMT2 regulates serine metabolism to promote the progression and immunosuppression of papillary renal cell carcinoma |
| title_full | SHMT2 regulates serine metabolism to promote the progression and immunosuppression of papillary renal cell carcinoma |
| title_fullStr | SHMT2 regulates serine metabolism to promote the progression and immunosuppression of papillary renal cell carcinoma |
| title_full_unstemmed | SHMT2 regulates serine metabolism to promote the progression and immunosuppression of papillary renal cell carcinoma |
| title_short | SHMT2 regulates serine metabolism to promote the progression and immunosuppression of papillary renal cell carcinoma |
| title_sort | shmt2 regulates serine metabolism to promote the progression and immunosuppression of papillary renal cell carcinoma |
| topic | cancer metabolism serine tumour microenvironment SHMT2 immunotherapy |
| url | https://www.frontiersin.org/articles/10.3389/fonc.2022.914332/full |
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