Elevated leukotriene B4 and 8-isoprostane in exhaled breath condensate from preterm-born infants
Abstract Background Inflammation and oxidative stress play a key role in the development of bronchopulmonary dysplasia (BPD), possibly contributing to persistent respiratory morbidity after preterm birth. We aimed to assess if inflammatory markers were elevated in exhaled breath condensate (EBC) of...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-08-01
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| Series: | BMC Pediatrics |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12887-023-04210-y |
| _version_ | 1797451453923590144 |
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| author | Rhea Urs Rubi Ni Chin Naomi Hemy Andrew C. Wilson J. Jane Pillow Graham L. Hall Shannon J. Simpson |
| author_facet | Rhea Urs Rubi Ni Chin Naomi Hemy Andrew C. Wilson J. Jane Pillow Graham L. Hall Shannon J. Simpson |
| author_sort | Rhea Urs |
| collection | DOAJ |
| description | Abstract Background Inflammation and oxidative stress play a key role in the development of bronchopulmonary dysplasia (BPD), possibly contributing to persistent respiratory morbidity after preterm birth. We aimed to assess if inflammatory markers were elevated in exhaled breath condensate (EBC) of infants born very prematurely (< 32 weeks gestation) at 12–16 corrected months of age, and if increased levels were associated with BPD diagnosis and respiratory morbidity. Methods EBC samples and respiratory questionnaires were collected from 15 term-born infants and 33 preterm-born infants, 12 with a neonatal BPD diagnosis. EBC samples were analysed for leukotriene B4 (inflammation) and 8-isoprostane (oxidative stress) concentrations using enzyme-linked immune-assays. Differences between groups were analysed by Kruskal-Wallis Test with post-hoc comparisons, independent samples t-test or Mann-Whitney U test depending on normality of the data. Results Leukotriene B4 and 8-isoprostane levels were elevated in exhaled breath condensate of preterm-born infants compared to those born at term (mean difference [95% CI]; 1.52 [0.45, 2.59], p = 0.02; 0.77 [0.52, 1.02], p < 0.001, respectively). Leukotriene B4 and 8-isoprostane levels were independent of BPD diagnosis and respiratory morbidity over the first year of life. Conclusions Infants born very prematurely exhibit elevated markers of airway neutrophilic inflammation and oxidative stress beyond the first year of life, regardless of a neonatal diagnosis of chronic lung disease or respiratory morbidity during infancy. These findings may have implications for future lung health. Trial Registration N/A. |
| first_indexed | 2024-03-09T14:54:52Z |
| format | Article |
| id | doaj.art-173e652457454ac3b499a31fe588d238 |
| institution | Directory Open Access Journal |
| issn | 1471-2431 |
| language | English |
| last_indexed | 2024-03-09T14:54:52Z |
| publishDate | 2023-08-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Pediatrics |
| spelling | doaj.art-173e652457454ac3b499a31fe588d2382023-11-26T14:15:04ZengBMCBMC Pediatrics1471-24312023-08-012311910.1186/s12887-023-04210-yElevated leukotriene B4 and 8-isoprostane in exhaled breath condensate from preterm-born infantsRhea Urs0Rubi Ni Chin1Naomi Hemy2Andrew C. Wilson3J. Jane Pillow4Graham L. Hall5Shannon J. Simpson6School of Allied Health, Curtin UniversityWal-yan Respiratory Centre, Telethon Kids InstituteWal-yan Respiratory Centre, Telethon Kids InstituteSchool of Allied Health, Curtin UniversityWal-yan Respiratory Centre, Telethon Kids InstituteSchool of Allied Health, Curtin UniversitySchool of Allied Health, Curtin UniversityAbstract Background Inflammation and oxidative stress play a key role in the development of bronchopulmonary dysplasia (BPD), possibly contributing to persistent respiratory morbidity after preterm birth. We aimed to assess if inflammatory markers were elevated in exhaled breath condensate (EBC) of infants born very prematurely (< 32 weeks gestation) at 12–16 corrected months of age, and if increased levels were associated with BPD diagnosis and respiratory morbidity. Methods EBC samples and respiratory questionnaires were collected from 15 term-born infants and 33 preterm-born infants, 12 with a neonatal BPD diagnosis. EBC samples were analysed for leukotriene B4 (inflammation) and 8-isoprostane (oxidative stress) concentrations using enzyme-linked immune-assays. Differences between groups were analysed by Kruskal-Wallis Test with post-hoc comparisons, independent samples t-test or Mann-Whitney U test depending on normality of the data. Results Leukotriene B4 and 8-isoprostane levels were elevated in exhaled breath condensate of preterm-born infants compared to those born at term (mean difference [95% CI]; 1.52 [0.45, 2.59], p = 0.02; 0.77 [0.52, 1.02], p < 0.001, respectively). Leukotriene B4 and 8-isoprostane levels were independent of BPD diagnosis and respiratory morbidity over the first year of life. Conclusions Infants born very prematurely exhibit elevated markers of airway neutrophilic inflammation and oxidative stress beyond the first year of life, regardless of a neonatal diagnosis of chronic lung disease or respiratory morbidity during infancy. These findings may have implications for future lung health. Trial Registration N/A.https://doi.org/10.1186/s12887-023-04210-yBronchopulmonary dysplasiaPretermInfantInflammation |
| spellingShingle | Rhea Urs Rubi Ni Chin Naomi Hemy Andrew C. Wilson J. Jane Pillow Graham L. Hall Shannon J. Simpson Elevated leukotriene B4 and 8-isoprostane in exhaled breath condensate from preterm-born infants BMC Pediatrics Bronchopulmonary dysplasia Preterm Infant Inflammation |
| title | Elevated leukotriene B4 and 8-isoprostane in exhaled breath condensate from preterm-born infants |
| title_full | Elevated leukotriene B4 and 8-isoprostane in exhaled breath condensate from preterm-born infants |
| title_fullStr | Elevated leukotriene B4 and 8-isoprostane in exhaled breath condensate from preterm-born infants |
| title_full_unstemmed | Elevated leukotriene B4 and 8-isoprostane in exhaled breath condensate from preterm-born infants |
| title_short | Elevated leukotriene B4 and 8-isoprostane in exhaled breath condensate from preterm-born infants |
| title_sort | elevated leukotriene b4 and 8 isoprostane in exhaled breath condensate from preterm born infants |
| topic | Bronchopulmonary dysplasia Preterm Infant Inflammation |
| url | https://doi.org/10.1186/s12887-023-04210-y |
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