Neuroprotection effects of ethyl acetate and n-butanol fractions of the hydroalcoholic extract of Tanacetum bodjnordens on sciatic nerve compression in male rats
Background and Aims: When a neuronal axon is damaged, it returns to the neuron cell body and destroys it. Tanacetum bodjnordens as antioxidant and anti-apoptotic effects. This study aimed to determine the neuroprotective effects of ethyl acetate and n-butanol and hydroalcoholic extracts of Tanacetum...
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| Format: | Article |
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Birjand University of Medical Sciences and Health Services
2021-06-01
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| Series: | مجله دانشگاه علوم پزشکی بیرجند |
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| Online Access: | http://journal.bums.ac.ir/article-1-2922-en.html |
| _version_ | 1818554025427075072 |
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| author | Mehri Ghahri Maryam Tehranipour Jina Khayatzadeh |
| author_facet | Mehri Ghahri Maryam Tehranipour Jina Khayatzadeh |
| author_sort | Mehri Ghahri |
| collection | DOAJ |
| description | Background and Aims: When a neuronal axon is damaged, it returns to the neuron cell body and destroys it. Tanacetum bodjnordens as antioxidant and anti-apoptotic effects. This study aimed to determine the neuroprotective effects of ethyl acetate and n-butanol and hydroalcoholic extracts of Tanacetum bodjnordens on sciatic nerve compression in male rats.
Materials and Methods: In this experimental study, 36 male Wistar rats weighing 200-250 g were randomly divided into 6 groups )n=6). In the control group, the right thigh muscle of the rats was split after the anesthetization of the rats, while in the compression and treatment groups, the sciatic nerve was compressed for 60 seconds. The plant extract was injected intraperitoneally on the day of compression and seven days later. After 28 days, samples were taken from the lumbar spinal cord subsequent to performing the perfusion method. Afterward, 7-μm serial sections were prepared and stained using toluidine blue stain after tissue passage. Eventually, the neuronal density of rats in the six groups was compared.
Results: Based on the results, the neuronal density in the compression group decreased significantly compared to controls and showed a significant increase in the hydroalcoholic, n-butanol, and aqueous phase treatment groups compared to that in the compression group (P<0.001).
Conclusion: According to the results, it seems that Tanacetum bodjnordens leaf extract has neuroprotective effects that promote the regeneration process in damaged neurons and these effects are higher in the aqueous phase fraction. |
| first_indexed | 2024-12-12T09:33:40Z |
| format | Article |
| id | doaj.art-1743cdf5c1c54c44b8ba29dd5ef5a8ce |
| institution | Directory Open Access Journal |
| issn | 1607-2197 2423-6152 |
| language | fas |
| last_indexed | 2024-12-12T09:33:40Z |
| publishDate | 2021-06-01 |
| publisher | Birjand University of Medical Sciences and Health Services |
| record_format | Article |
| series | مجله دانشگاه علوم پزشکی بیرجند |
| spelling | doaj.art-1743cdf5c1c54c44b8ba29dd5ef5a8ce2022-12-22T00:28:47ZfasBirjand University of Medical Sciences and Health Servicesمجله دانشگاه علوم پزشکی بیرجند1607-21972423-61522021-06-01282120128Neuroprotection effects of ethyl acetate and n-butanol fractions of the hydroalcoholic extract of Tanacetum bodjnordens on sciatic nerve compression in male ratsMehri Ghahri0Maryam Tehranipour1Jina Khayatzadeh2 Student Research Committee, Department of Biology, Faculty of Science, Mashhad Branch, Islamic Azad University PhD of Animal Physiology, Department of Biology, Faculty of Science, Mashhad Branch, Islamic Azad University PhD in Animal Science, Department of Biology, Faculty of Science, Mashhad Branch, Islamic Azad University Background and Aims: When a neuronal axon is damaged, it returns to the neuron cell body and destroys it. Tanacetum bodjnordens as antioxidant and anti-apoptotic effects. This study aimed to determine the neuroprotective effects of ethyl acetate and n-butanol and hydroalcoholic extracts of Tanacetum bodjnordens on sciatic nerve compression in male rats. Materials and Methods: In this experimental study, 36 male Wistar rats weighing 200-250 g were randomly divided into 6 groups )n=6). In the control group, the right thigh muscle of the rats was split after the anesthetization of the rats, while in the compression and treatment groups, the sciatic nerve was compressed for 60 seconds. The plant extract was injected intraperitoneally on the day of compression and seven days later. After 28 days, samples were taken from the lumbar spinal cord subsequent to performing the perfusion method. Afterward, 7-μm serial sections were prepared and stained using toluidine blue stain after tissue passage. Eventually, the neuronal density of rats in the six groups was compared. Results: Based on the results, the neuronal density in the compression group decreased significantly compared to controls and showed a significant increase in the hydroalcoholic, n-butanol, and aqueous phase treatment groups compared to that in the compression group (P<0.001). Conclusion: According to the results, it seems that Tanacetum bodjnordens leaf extract has neuroprotective effects that promote the regeneration process in damaged neurons and these effects are higher in the aqueous phase fraction.http://journal.bums.ac.ir/article-1-2922-en.htmldegenerationneuronal densityneuroprotectivetanacetum bodjnordens |
| spellingShingle | Mehri Ghahri Maryam Tehranipour Jina Khayatzadeh Neuroprotection effects of ethyl acetate and n-butanol fractions of the hydroalcoholic extract of Tanacetum bodjnordens on sciatic nerve compression in male rats مجله دانشگاه علوم پزشکی بیرجند degeneration neuronal density neuroprotective tanacetum bodjnordens |
| title | Neuroprotection effects of ethyl acetate and n-butanol fractions of the hydroalcoholic extract of Tanacetum bodjnordens on sciatic nerve compression in male rats |
| title_full | Neuroprotection effects of ethyl acetate and n-butanol fractions of the hydroalcoholic extract of Tanacetum bodjnordens on sciatic nerve compression in male rats |
| title_fullStr | Neuroprotection effects of ethyl acetate and n-butanol fractions of the hydroalcoholic extract of Tanacetum bodjnordens on sciatic nerve compression in male rats |
| title_full_unstemmed | Neuroprotection effects of ethyl acetate and n-butanol fractions of the hydroalcoholic extract of Tanacetum bodjnordens on sciatic nerve compression in male rats |
| title_short | Neuroprotection effects of ethyl acetate and n-butanol fractions of the hydroalcoholic extract of Tanacetum bodjnordens on sciatic nerve compression in male rats |
| title_sort | neuroprotection effects of ethyl acetate and n butanol fractions of the hydroalcoholic extract of tanacetum bodjnordens on sciatic nerve compression in male rats |
| topic | degeneration neuronal density neuroprotective tanacetum bodjnordens |
| url | http://journal.bums.ac.ir/article-1-2922-en.html |
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